Genetic Diversity of Cultivated Pears from Fujian Province Revealed by cpDNA Haplotypes and Nuclear Microsatellites

doi:10.16420/j.issn.0513-353x.2014-0583

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (1): 119-130.doi: 10.16420/j.issn.0513-353x.2014-0583

• Research Notes • Previous Articles Next Articles

Genetic Diversity of Cultivated Pears from Fujian Province Revealed by cpDNA Haplotypes and Nuclear Microsatellites

YUE Xiao-yan1，HUANG Xin-zhong2，ZONG Yu1，and TENG Yuan-wen

1Department of Horticulture，the State Agricultural Ministry’s Key Laboratory of Horticultural Plant Growth，Development & Quality Improvement，Zhejiang University，Hangzhou 310058，China；2Fruit Research Institute，Fujiang Academy of Agricultural Sciences，Fuzhou 350013，China

Online:2015-01-25 Published:2015-01-25

Abstract

Abstract: A non-coding chloroplast region，accD-psaI and 17 microsatellite markers were used to assay genetic diversity of 49 pear accessions from Fujian Province. A total of 49 sequences were collected and five haplotypes（Hap1–Hap5）were recognized containing four variation sites. Nucleotide diversity（Pi）and haplotype diversity were 0.00139 and 0.660. The TCS Network indicated that Hap5 was the ancestral haplotype，only detected in two individuals. Two hundred and eleven different alleles were detected among seventeen SSR loci，ranged from 5 to 23 with an average of 12.41 alleles per locus. The mean observed and expected heterozygosity values were 0.5802 and 0.7549，respectively. The Shannon’s information index for each locus was from 0.5830 to 2.7683 with a mean value of 1.8661. The Neighbor-Joining（NJ）tree grouped 49 pear accessions to nine major groups，which are generally congruent to haplotype diversity. Chloroplast haplotypes and microsatellite locus polymorphism information demonstrated a high level of genetic diversity of pear landraces from Fujian，China.

Key words: pear, landraces, SSR, cpDNA, genetic diversity, genetic relationship

CLC Number:

S 661.2

YUE Xiao-yan1，HUANG Xin-zhong2，ZONG Yu1，and TENG Yuan-wen. Genetic Diversity of Cultivated Pears from Fujian Province Revealed by cpDNA Haplotypes and Nuclear Microsatellites[J]. ACTA HORTICULTURAE SINICA, 2015, 42(1): 119-130.

[1]	WANG Rui, HONG Wenjuan, LUO Hua, ZHAO Lina, CHEN Ying, and WANG Jun, . Construction of SSR Fingerprints of Pomegranate Cultivars and Male Parent Identification of Hybrids [J]. Acta Horticulturae Sinica, 2023, 50(2): 265-278.
[2]	WANG Mengmeng, SUN Deling, CHEN Rui, YANG Yingxia, ZHANG Guan, LÜ Mingjie, WANG Qian, XIE Tianyu, NIU Guobao, SHAN Xiaozheng, TAN Jin, and YAO Xingwei, . Construction and Evaluation of Cauliflower Core Collection [J]. Acta Horticulturae Sinica, 2023, 50(2): 421-431.
[3]	LIU Yiping, NI Menghui, WU Fangfang, LIU Hongli, HE Dan, KONG Dezheng. Association Analysis of Organ Traits with SSR Markers in Lotus（Nelumbo nucifera） [J]. Acta Horticulturae Sinica, 2023, 50(1): 103-115.
[4]	SONG Jiankun, YANG Yingjie, LI Dingli, MA Chunhui, WANG Caihong, and WANG Ran. A New Pear Cultivar‘Luxiu’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 3-4.
[5]	DONG Xingguang, CAO Yufen, ZHANG Ying, TIAN Luming, HUO Hongliang, QI Dan, XU Jiayu, LIU Chao, and WANG Lidong. A New Cold-resistant Crispy Pear Cultivar‘Yucuixiang’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 5-6.
[6]	OU Chunqing, JIANG Shuling, WANG Fei, MA Li, ZHANG Yanjie, and LIU Zhenjie. A New Early-ripening Pear Cultivar‘Xingli Mishui’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 7-8.
[7]	ZHANG Yanjie, WANG Fei, OU Chunqing, MA Li, JIANG Shuling, and LIU Zhenjie. A New Pear Cultivar‘Zhongli Yucui 3’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 9-10.
[8]	WANG Suke, LI Xiugen, YANG Jian, WANG Long, SU Yanli, ZHANG Xiangzhan, and XUE Huabai. A New Red Pear Cultivar‘Danxiahong’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 13-14.
[9]	WANG Fei, OU Chunqing, ZHANG Yanjie, MA Li, and JIANG Shuling. A New Late Ripening Pear Cultivar‘Huaqiu’with Long Storage Period [J]. Acta Horticulturae Sinica, 2022, 49(S1): 9-10.
[10]	SONG Jiankun, LI Dingli, YANG Yingjie, MA Chunhui, WANG Caihong, and WANG Ran. A New Pear Cultivar‘Qindaohong’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 11-12.
[11]	GUO Weizhen, ZHAO Jingxian, LI Ying. A New Mid-early Ripening Pear Cultivar‘Meiyu’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2051-2052.
[12]	LIU Jinming, GUO Caihua, YUAN Xing, KANG Chao, QUAN Shaowen, NIU Jianxin. Genome-wide Identification of Dof Family Genes and Expression Analysis Sepal Persistent and Abscission in Pear [J]. Acta Horticulturae Sinica, 2022, 49(8): 1637-1649.
[13]	TAO Xin, ZHU Rongxiang, GONG Xin, WU Lei, ZHANG Shaoling, ZHAO Jianrong, ZHANG Huping. Fructokinase Gene PpyFRK5 Plays an Important Role in Sucrose Accumulation of Pear Fruit [J]. Acta Horticulturae Sinica, 2022, 49(7): 1429-1440.
[14]	LI Wenting, LI Cuixiao, LIN Xiaoqing, ZHENG Yongqin, ZHENG Zheng, DENG Xiaoling. Population Genetic Structure of Xanthomonas citri pv. citri in Guangdong Province Based on the STR Locus [J]. Acta Horticulturae Sinica, 2022, 49(6): 1233-1246.
[15]	ZHANG Meng, SHAN Yuying, YANG Yebo, ZHAI Feifei, WANG Zhaoshan, JU Guansheng, SUN Zhenyuan, LI Zhenjian. AFLP Analysis of Genetic Resources of Dendrobium from China [J]. Acta Horticulturae Sinica, 2022, 49(6): 1339-1350.

Genetic Diversity of Cultivated Pears from Fujian Province Revealed by cpDNA Haplotypes and Nuclear Microsatellites

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments