关键词: 红叶桃, 荧光定量PCR, 花色苷生物合成, 基因表达

Abstract:

The leaves of‘Zhubo-5’peach in vitro were as the test materials，using the fluorescent
quantitative PCR technology to analysis related gene expression level of anthocyanins biosynthesis via
different concentrations of NaCl，sucrose and KH2PO4 treatment. The result showed，NaCl treatment can
improve the expression level of CHI，UFGT，MYB10，bHLH33 genes，and suppress MYB15 and WD40
genes expression. For CHI gene，sucrose and KH2PO4 treatment can significantly improve its expression，
and within a certain range，the expression levels were positively correlated with concentration and time. The
expression level of UFGT in three concentrations of sucrose began to increase after 8 h，the concentration
of 0.3% was the best. MYB10 gene transcription level increased by sugar processing，consistent with the
CHI. Concentration of 0.8% sucrose treatment decreased bHLH33 expression，concentration of 0.3% and 0.5%，after 8 h showed positive regulation function，and in 0.3% 8 h was the best effect. KH2PO4 treatment
improved UFGT，MYB10，bHLH33 and WD40 expression at 0.2%，and the peak-expression was after 8 h
treatment. In this experiment，sucrose was inhibitory effected on WD40 gene. The result of this study was
to further clarify the mechanism of anthocyanins synthesis and provide certain theoretical basis to improve
ornamental period.

Key words: Prunus persica f. alropurpurea, fluorescence quantitative PCR, anthocyanins biosynthesis, gene expression