关键词: 菜薹, 抽薹, 基因结构, 表达

Abstract: In order to predict the function of BrcuDFR-like/BrcuAXS，both the full-length of cDNA and genomic DNA were cloned with the method of PCR and RACE and expression pattern was investigated in flowering Chinese cabbage（Brassica rapa syn. campestris L. ssp. chinensis var. utilis Tsen et Lee）. The results indicated that the cDNA with the complete coding region was 1 332 bp in length which encoded 312 putative amino acids. The corresponding gDNA was 2 460 bp in length which harbored eight exons and seven introns. The longest intron was the third intron with 401 bp in length. A computer scan disclosed that the introns harbored light-responsive element，gibberellin-responsive element，defense and stress responsiveness element，heat stress responsiveness element，WRKY and MYB binding site and so on. The semi-quantitative RT-PCR analysis revealed that no detectable levels were expressed during the first stage of sampling，then transcripts were detected during the two true-leaf，the four true-leaf，the five true-leaf and the flowering stages. Based on expression analysis，it is more likely to encode DFR than AXS，and it may play a role of transition from vegetative growth to reproductive growth in flowering Chinese cabbage.

Key words: flowering Chinese cabbage, bolting, gene structure, expression