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园艺学报 ›› 2001, Vol. 28 ›› Issue (2): 128-132.

• 研究论文 • 上一篇    下一篇

乙烯受体基因LeETR1在番茄突变体Epi及其野生型中的表达

郑铁松1;应铁进1*;何国庆1;曹家树2   

  1. (1 浙江大学食品科学与营养系, 杭州310029; 2 浙江大学园艺系, 杭州310029)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-04-25 发布日期:2001-04-25

Expression of Ethylene Receptor Gene LeETR1 in Tomato Mutant Epi and Its Wild Type

Zheng Tiesong1, Ying Tiejin1, He Guoqing1, and Cao Jiashu2   

  1. (1Department of Food Science and Nutriology , Zhej iang University , Hangz hou 310029; 2Department of Horticulture, Zhejiang University , Hangzhou 310029)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-04-25 Published:2001-04-25

摘要: 采用核酸酶保护分析( RPA) 方法对番茄乙烯过表达单基因突变体Epi 和野生型VFN8 中LeETR1 mRNA 的表达特征进行了研究。结果表明, 在正常番茄中LeETR1 mRNA 不受内源乙烯含量的影响, 呈组成性表达。LeETR1 mRNA 在Epi 部分组织中的表达强度发生了改变, 并与Epi 的形态特征变化相吻合, 提示LeETR1 在叶片的形态建成、果实成熟和顶钩发育中可能起着重要的作用。

关键词: 番茄, 乙烯受体, LeETR1, 核酸酶保护分析, 基因

Abstract: The expressive characteristics of LeETR1 mRNA in tomato mutant Epi and its wild type VFN8 have been studied with RNase protect assay ( RPA) . The results showed that LeETR1 mRNA expressed constitutionally in VFN8. And its expression was not affected by the endogenous production of ethylene. However, the high expression of LeETR1 in some tissues of Epi suggested that LeETR1 gene may have an important role in the morphogenesis of leaves, apical hooks and fruit ripening in tomato.

Key words: Tomato, Ethylene receptor, LeETR1, RPA, Gene

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