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园艺学报 ›› 2002, Vol. 29 ›› Issue (4): 367-368.

• 研究报告 • 上一篇    下一篇

梨叶片离体培养和植株再生

徐凌飞1;马锋旺1;王 之2;任小林1;曹晓雁2   

  1. (1 西北农林科技大学园艺学院, 杨凌712100 ; 2 陕西师范大学生命科学学院, 西安710062)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-08-25 发布日期:2002-08-25

Leaf Culture and Plantlet Regeneration of Pear (Pyrus)

Xu Lingfei1, Ma Fengwang1, Wang Zhezhi2, Ren Xiaolin1, and Cao Xiaoyan2   

  1. (1 College of Horticulture , Northwest Sci-Tec University of Agriculture and Forestry , Yangling 712100 , China ; 2College of life Science , Shaanxi Normal University , Xi’an 710062 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

摘要: 用梨(Pyrus) 4 个栽培品种的叶片诱导不定芽。暗培养20 d , 取试管苗顶部幼嫩叶片, 远轴
面向下接触培养基, 叶片再生效率高。八月红品种的叶片再生效率高于其它3 个品种。在NN69 + TDZ 1. 0 mg/L + IBA 0. 5 mg/L 培养基上, 八月红梨叶片再生频率为100 % , 平均再生芽数为5. 78 。八月红梨和水晶梨叶片再生苗在附加IBA 的1/ 2MS 培养基上生根。

关键词: 梨, 叶片培养, 植株再生

Abstract: Adventitious bud was regenerated from in vitro leaf culture of four pear scion cultivars. 20 days of dark culture was conducted , it was proved that using leaf of upper part of the plantlet as explant , and plating leaf
with abaxial layer downside may significantly enhanced the regeneration efficiency of leaf . The regeneration frequency and mean number of bud per leaf of‘Bayuehong’pear were 100 % and 5. 78 on NN69 + TDZ 1. 0 mg/ L + IBA 0. 5 mg/ L. The regenerated plants of‘Bayuehong’and‘Suisho’pear rooted in the 1/ 2 MS media supplemented with IBA.

Key words: Pear, Leaf culture, Plantlet regeneration

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