园艺学报 ›› 2009, Vol. 36 ›› Issue (3): 327-332.

• 果树 • 上一篇    下一篇



  1. 1烟台农业科学研究院果树所,山东烟台265500;2中国农业大学园艺植物研究所,北京 100094)
  • 收稿日期:2008-10-17 修回日期:2009-02-20 出版日期:2009-03-25 发布日期:2009-03-25
  • 通讯作者: 李元军;唐美玲;韩振海

Optimization of AFLP System for Fuji and Identification Early-maturing Sport from Changfu 2’

LI Yuan-jun1*,TANG Mei-ling1*,YU Qing1, LIU Mei-ying1,SONG Lai-qing1, and HAN Zhen-hai2**


  1. (1Fruit Institute of Yantai Agricultural Science Academy, Yantai, Shandong 265500,China; 2Institute of Horticultural Plants,China Agricultural University,Beijing 100094,China)
  • Received:2008-10-17 Revised:2009-02-20 Online:2009-03-25 Published:2009-03-25
  • Contact: LI Yuan-jun;TANG Mei-ling;HAN Zhen-hai


以‘长富2号’苹果为材料,采用优化的AFLP技术对其早熟芽变与母株进行初步研究,筛选了64对引物,其中43对引物扩增结果理想,共产生了2,700条带。有4对引物在芽变与母株之间产生了7条差异片段,主要集中在350~800 bp之间。研究表明早熟芽变是由于遗传物质改变造成的,同时也说明利用该体系对苹果芽变进行分析和鉴定获得成功。

关键词: 苹果, AFLP, 芽变

Abstract: Using AFLP to compare the early-maturing sport and its mother plant in ‘Changfu 2’.Sixty-four selective primer pairs was adopted in AFLP analysis. 2,700 clear bands was detected by 43 pairs of AFLP primers. There were 7 obvious bands difference between early-maturing sport and mother plant under 4 pair primers. The molecular weight range of the polymorphic fragments was mainly 350~800 bp.The results indicated that there were some molecular-level differentiation between 'Changfu 2' and its early-maturing sport. The application of AFLP in the research of apple bud mutation is Successfu1.

Key words: apple, AFLP, bud mutation