园艺学报 ›› 2005, Vol. 32 ›› Issue (02): 295-297.

• 研究报告 • 上一篇    下一篇



  1. (1 中国农业大学园艺植物研究所, 北京100094; 2 弘前大学农学生命科学部, 日本弘前03628561)
  • 收稿日期:2004-05-12 修回日期:2004-08-07 出版日期:2005-04-25 发布日期:2005-04-25

Observation on Structural Changes of Self-pollinated Pollen Tube and Localization of S-RNase in Apple

Li Tianzhong1;Toshikatsu Okuno2   

  1. (1 Institute for Horticultural Plants, China Agricultural University, Beijing 100094, China; 2 Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 03628561, Japan)
  • Received:2004-05-12 Revised:2004-08-07 Online:2005-04-25 Published:2005-04-25

摘要: 苹果‘红星’品种授粉处理24 h后, 自花授粉比异花授粉的花粉管生长缓慢。授粉处理48 h后, 可观察到自花授粉花柱通道组织的花粉管内有二裂粒子增加、管壁破裂和管内物质溢出、外层管壁增厚等现象。苹果气球状花期未授粉的花柱及自花和异花授粉24 h后且无花粉管存在的花柱切片显示, S-RNase位于花柱通道组织的细胞内。自花和异花授粉48 h后有花粉管通过的花柱切片显示, S-RNase位于花柱通道组织的细胞间隙和花粉管内。

关键词: 苹果, 自花授粉, 花粉管, 结构变化, S-RNase, 定位

Abstract: Structural changes in the pollen tubes of the ‘Starking Delicious’apple in vivo was investigated by transmission electron microscope. Itwas found that a slow growth of the pollen tube after 24 h for the self-pollination. Compared with the cross-pollination, there were numerous bipartite particles in the pollen tube cell and some changes in 48 h for the self-pollination, either appeared such as rupture of the pollen tube, release of the cellularmaterials, thickening of the outer walls of some pollen tube cells. At the time without pollination, or 24 h after self- and cross-pollination, in the samp les that pollen tubes did not exist, the S-RNase was localized in the stylar transmitting tissue cells. However, in 48 h after pollination with self- or cross-pollen, in the samp les that pollen tubes existed, the S-RNase was observated in the intercellular space or into pollen tubes.

Key words: Apple, Self-pollination, Pollen tube, Structural change, S-RNase, Localization