园艺学报 ›› 2021, Vol. 48 ›› Issue (12): 2360-2374.doi: 10.16420/j.issn.0513-353x.2020-0911

• 研究论文 • 上一篇    下一篇


侯黔东1, 沈天娇1, 余欢欢1, 仇志浪1, 文壮1, 张惠敏1,2, 吴亚维3, 文晓鹏1,*()   

  1. 1贵州大学生命科学学院,农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳 550025
    2贵州大学林学院,贵州省森林资源与环境研究中心,贵阳 550025
    3贵州省农业科学院果树研究所,贵阳 550006
  • 收稿日期:2021-01-06 修回日期:2021-05-06 发布日期:2022-01-04
  • 通讯作者: 文晓鹏
  • 基金资助:

Genome-wide Identification and Expression Analysis of Prunus avium Gretchen Hagen 3(GH3)Gene Family

HOU Qiandong1, SHEN Tianjiao1, YU Huanhuan1, QIU Zhilang1, WEN Zhuang1, ZHANG Huimin1,2, WU Yawei3, WEN Xiaopeng1,*()   

  1. 1Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering(CICMEAB),Institute of Agro-bioengineering,College of Life Sciences,Guizhou University,Guiyang 550025,China
    2Institute for Forest Resources & Environment of Guizhou,College of Forestry,Guizhou University,Guiyang 550025,China
    3Institute of Fruit Tree Research,Guizhou Provincial Academy of Agricultural Sciences,Guiyang 550006,China
  • Received:2021-01-06 Revised:2021-05-06 Published:2022-01-04
  • Contact: WEN Xiaopeng


以甜樱桃(Prunus avium L.)为材料,对IAA酰胺合成酶(Gretchen Hagen 3,GH3)基因家族全基因组进行鉴定及生物信息分析;同时,分析其在不同组织中的表达及对外源GA3、ABA、MeJA、IAA的响应。结果表明,甜樱桃基因组中共存在8个GH3基因,根据在染色体的位置依次命名为PavGH3.1 ~ PavGH3.8,编码区长度为1 683 ~ 1 851 bp,预测大多数基因定位在叶绿体中;PavGH3外显子数3 或 4,且有较多保守基序;除5号和7号染色体外,其他染色体均有分布;启动子存在ABA、MeJA等6种激素响应元件;PavGH3在进化上分为2组,且PavGH3.5PavGH3.6与拟南芥GH3一些成员存在共线性。表达分析显示,PavGH3.2PavGH3.6PavGH3.7为3个主要表达基因。PavGH3.4PavGH3.5在第2次生理落果中较第1次上调表达,与正常果实相比,第1次生理落果中PavGH3.5PavGH3.7下调表达,PavGH3.4PavGH3.6上调表达。PavGH3.2PavGH3.3PavGH3.6PavGH3.7为主要响应激素的基因。推测PavGH3基因家族广泛参与甜樱桃的生长发育,且可能与落果关系密切。

关键词: 甜樱桃, IAA酰胺合成酶, 基因家族, 生长素响应, 表达分析


Auxin plays a crucial role in regulating the growth and development of plants. The acyl acid amido synthetase Gretchen Hagen 3(GH3)may modulate auxin levels via conjugting both indole-3-acetic acid(IAA)and salicylic acid(SA)in response to environment changes. In the current investigation,genome-wide identification and comprehensive analysis of the GH3 gene family in sweet cherry(Prunus avium)were conducted using the genomic sequence. Totally,eight members of PavGH3 genes were identified from the whole genome of sweet cherry,which are not evenly distributed on the chromosomes,with the coding sequence of 1 683-1 851 bp,most of which were forecasted in chloroplast. The analysis of gene structure and domain revealed that PavGH3 demonstrated high similarity among the members,and the number of exons was three or four. A total of 16 conserved domains were obtained,and the number of the conserved domains varied among the members. These gene members were clustered into two major groups(I and II),based on phylogenetic analysis. Sequence analysis of promoters showed many cis-regulatory elements which presumably responsed to phytohormones such as abscisic acid,methyl jasmonate etc. Synteny block indicated that PavGH3.5 and PavGH3.6 showed collinearity with GH3 in Arabidopsis thaliana,suggesting that they were evolutionarily conserved. Multiple sequence alignment demonstrated that these members are highly similar and conserved. As detected by qRT-PCR in 17 tissues. PavGH3.2,PavGH3.3 and PavGH3.6 were highy expressed in these tissues. PavGH3.4 and PavGH3.5 were up-regulated in the dropped-fruit of the second physiological abscission in comparison with that of the first one. Compared with retention fruits,surprisingly,PavGH3.5 and PavGH3.7 were significantly down-regulated in the first physiological fruit drops,PavGH3.4 and PavGH3.6 were up-regulated. IAA,GA3,ABA and MeJA were used to foliar application,and PavGH3.2,PavGH3.3,PavGH3.6 and PavGH3.7 were differentially expressed in response to the type and application stage of the phytohormones,however,no responses were observed in the others. Therefore,it is speculated that the PavGH3 gene family is considerably involved in the regulation of the growth and development of sweet cherry,and probably in the fruit abscission.

Key words: Prunus avium, Gretchen Hagen 3, gene family, auxin response, expression analysis