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园艺学报 ›› 2018, Vol. 45 ›› Issue (6): 1204-1212.doi: 10.16420/j.issn.0513-353x.2017-0521

• 研究报告 • 上一篇    下一篇

环割对柑橘叶片衰老的影响

梁春辉1,2,陈惠敏1,李 娟3,贺世雄1,陈杰忠1,*,姚 青1   

  1. 1华南农业大学园艺学院,广州 510642;2广东农工商职业技术学院,广州 510507;3仲恺农业工程学院园艺园林学院,广州 510225
  • 出版日期:2018-06-25 发布日期:2018-06-25
  • 基金资助:
    国家自然科学基金项目(31372008);广东省科技计划项目(2014A020209081,2014A020208085,2015A020209110,2016LM3174);广东省高等学校优秀青年教师培养计划项目(Yq2013095)

Effects of Girdling on Senescence of Citrus Leaf

LIANG Chunhui1,2,CHEN Huimin1,LI Juan3,HE Shixiong1,CHEN Jiezhong1,*,and YAO Qing1   

  1. 1College of Horticulture,South China Agricultural University,Guangzhou 510642,China;2Guangdong Agriculture Industry Business Polytechnic College,Guangzhou 510507,China;3Department of Horticulture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China
  • Online:2018-06-25 Published:2018-06-25

摘要: 以3年生红黎檬砧‘暗柳橙’(Anliucheng)为试材,研究了环割与环剥对柑橘叶片光合性能及活性氧代谢的影响。结果表明,环割和环剥30 d后均不同程度降低了叶片叶绿素相对含量(SPAD)、净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和细胞间CO2浓度(Ci),其中环剥处理对叶片SPAD和Pn产生了持续性的影响;环割和环剥在处理的前期均显著增加了叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化酶活性;处理30 d后叶片抗氧化物质抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量显著低于对照,随后呈上升趋势,达到一个峰值后缓慢恢复到正常状态;各处理叶片膜脂过氧化产物丙二醛(MDA)含量均呈上升趋势,环割2次和环剥处理叶片初期MDA含量显著高于对照,引发柑橘叶片中一系列抗氧化反应,造成其膜脂氧化程度加剧。

关键词: 柑橘, 环割, 光合作用, 活性氧

Abstract: The 3-year-old‘Anliu’orange(Citrus sinensis Osbeck)grafted on Honglimeng(Citrus limonia Osbeck)was used as experimental material to study the effect of girdling on photosynthetic property and reactive oxygen metabolism of Citrus leaves. The results showed that ringing and sprial girdling decreased the chlorophyll relative content(SPAD),net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs)and intercellular CO2 concentration(Ci)of leaves with different degrees after 30-day treatment. Girdling treatment had a long-term impact on leaf SPAD and Pn. The activities of antioxidant enzymes(SOD,POD and CAT)in leaves were improved after ringing and girdling. The antioxidant compounds(GSH and AsA)contents in leaves were significantly decreased at 30 d after treatment,then began rising to a peak and returned to a normal state slowly at last. The malondialdehyde (MDA)content was increased in all treatments,which was significantly increased compared with the control by ringing twice and girdling. These data showed that ringing and girdling in early stage affected photosynthetic metabolism of citrus leaves,and caused a series of antioxidation reaction and accumulation of reactive oxygen species in citrus leaves.

Key words: citrus, girdling, photosynthesis, reactive oxygen

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