关键词: 苹果, CRF, 不定根发育, 生物信息学

Abstract:

In the current study，a total of 11 cytokinin response factor genes（CRF）were identified from the apple genome by bioinformatics. Genes were named MdCRF1–MdCRF11 by the position of the gene in the chromosome. Additionally，this study systematically analyzed its physical and chemical characteristics，gene structures，evolutionary relationships and promoter elements. MdCRF proteins contained 137–435 amino acids and the isoelectric points is from 4.26 to 9.68. Results of exon-intron structure analysis showed that the MdCRF1 contained 2 exons and 1 intron；MdCRF11 contained 3 exons and 2 introns；other genes only have exons and without introns. Conservative base sequence analysis showed that 5–9 conserved motifs existed in the MdCRF proteins. Phylogenetic analysis showed that MdCRF family genes could be classified into three subgroups（G1，G2 and G3）according to its homology. In addition，the number of the members of each subgroup were approximately equal. Tissue-specific expressions indicated that 11 MdCRF had significantly different expression patterns in different genotypes，as well as different tissues. The materials were‘T337’apple rootstock，qPCR confirmed most of genes were highly expressed in root，stem and callus. At the same time the expression of these genes were down-regulated by rooting treatment. Additionally，we systematically analyzed the connection network of MdCRF proteins and predicted the potential link between genes. The results showed that MdCRF8，MdCRF10 and MdCRF11 might be involved in regulating adventitious root development in apple rootstock.

Key words: apple, CRF, adventitious root development, bioinformatic