关键词: 葡萄, SPL9, SPL10, 亚细胞定位, 荧光定量RT-PCR

Abstract: Firstly cloned two full length cDNAs of SPL9 and SPL10 SBPs（Squamosa promoter binding proteins）transcription factors from Summer Black（Vitis vinifera × V. labrusca）with accession No. of HM018600 and HM018601 deposited in GenBank database. The amino acid sequences of both genes had a putative nuclear localization signal sequence（KKSR），highly conserved SBP domains and the grapevine microRNA156a（Vv-miR156a）recognition sites. The sub-cellular location analysis using expression vectors of SPL9 and SPL10 constructed showed that they were both located in nucleus. The semi-quantitative and fluorescent quantitative RT-PCR were used to detect expression levels of Vv-SPL9，Vv-SPL10 and miR156a in different tissues of grapevine. The result showed that the two genes were expressed ubiquitously，but at different levels in different tissues. Their expression levels were the highest in small fruit，where miR156a was accumulated at a lowest level. The expression levels of the two genes showed some trade-off correlation with those of Vv-miRNA156a in various grapevine tissues，especially in the small fruit.

Key words: grapevine, SPL9, SPL0, sub-cellular localization, fluorescent quantitative RT-PCR