园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 195-200.

• 果树 • 上一篇    下一篇


唐美玲1,2; 孔 瑾1 ;许雪峰1; 沈育杰3;王 忆1;李天忠1 ;韩振海1﹡


  1. 1中国农业大学园艺植物研究所,北京 100094;2烟台农业科学院果树研究所,山东烟台 265200;3中国农业科学院家特产研究所,吉林左家 132019)
  • 收稿日期:2007-06-07 修回日期:2007-12-04 出版日期:2008-02-25 发布日期:2008-02-25
  • 通讯作者: 韩振海

Screening of the AFLP Markers Associated to the Sex Locus of Vitis amurensis Rupr. and its Conversion to SCAR Marker

TANG Mei-ling1,2,KONG Jin1, XU Xue-feng1,SHEN Yu-jie3,WANG Yi1,LI Tian-zhong1,and HAN Zhen-hai1﹡

  1. (1Institute of Horticultural Plants,China Agricultural University,Beijing 100094,China; 2Fruit Institute of Yantai Agricultural Science Academy,Yantai,Shandong 265200,China; 3 The Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, ZuoJia,Jilin, 132019,China)
  • Received:2007-06-07 Revised:2007-12-04 Online:2008-02-25 Published:2008-02-25
  • Contact: HAN Zhen-hai


山葡萄是一种重要的抗寒、抗病葡萄种质资源。通过AFLP分子标记方法,应用64对引物组合EcoR I-NNN/Mse I-NNN对山葡萄雌花、雄花和完全花植株基因池进行筛选,引物E-AGC/M-CTG在雄花和完全花植株基因池DNA中扩增出一条分子量为283 bp的特异性条带;经组池个体和其它品系验证,该特异性条带能在雄株和完全植株中稳定出现。将该特异性条带回收、克隆、测序,设计SCAR标记引物,对已知性别的85份山葡萄种质进行盲测,准确率达到97.6%,表明标记转化成功。由于该SCAR标记在绝大多数山葡萄栽培品种和有育种价值的种质资源中得到验证,可利用该SCAR标记对山葡萄杂交后代在苗期进行性别鉴定,提高育种效率。

关键词: 山葡萄, AFLP, SCAR, 分子标记

Abstract: Due to its resistant characters to cold, insect and disease, Vitis amurensis Rupr. is one of the most important germplasm resource in grape breeding. It has male, female and hermaphrodite phenotype, but only hermaphrodite is suitable for commercial production. AFLP(amplifled fragment length polymorphism) was applied to identify markers associated with Vitis amurensis Rupr. sex type. By application of 64 markers in the screening, E-AGC/M-CTG was found to amplify a specific 283bp fragment in male and hermaphrodite pool. According to its sequencing result, SCAR (Sequence Characterized Amplified Region) primers were designed, and the primers gave the expected band in 97.6% of the tested 85 cultivars of Vitis amurensis Rupr.. It suggests that the SCAR marker will be useful in the seedling stage marker-assistant selection for breeding with the resistant Vitis amurensis Rupr.

Key words: Vitis amurensis Rupr., AFLP, SCAR, Molecular marker