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园艺学报 ›› 2021, Vol. 48 ›› Issue (7): 1295-1303.doi: 10.16420/j.issn.0513-353x.2021-0142

• 研究论文 • 上一篇    下一篇

白菜与甘蓝型油菜—萝卜F染色体附加系回交BC1中萝卜F染色体的精准鉴定

丁云花1, Budahn Holger2, 赵泓1, 赵岫云1,*()   

  1. 1北京市农林科学院蔬菜研究中心,农业农村部华北地区园艺作物生物学与种质创制重点实验室,蔬菜种质改良北京市重点实验室,北京 100097
    2德国JKI园艺作物育种研究所,Quedlinburg D-06484
  • 收稿日期:2021-02-03 修回日期:2021-05-21 出版日期:2021-07-25 发布日期:2021-08-10
  • 通讯作者: 赵岫云 E-mail:zhaoxiuyun@nercv.org
  • 基金资助:
    国家自然科学基金项目(31672152);国家重点研发计划项目(2016YFD0100204-15);北京市农林科学院科技创新能力建设专项(KJCX20200113)

Accurate Identification of Radish Chromosome F in the Backcross Progeny of Brassica rapa and Rape-radish Chromosome F Addition Line

DING Yunhua1, Budahn Holger2, ZHAO Hong1, ZHAO Xiuyun1,*()   

  1. 1Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China
    2Julius Kühn-Institute,Institute for Breeding Research on Horticultural Crops,Quedlinburg D-06484,Germany
  • Received:2021-02-03 Revised:2021-05-21 Online:2021-07-25 Published:2021-08-10
  • Contact: ZHAO Xiuyun E-mail:zhaoxiuyun@nercv.org

摘要:

参考萝卜染色体定位序列标记连锁图谱中F染色体连锁群的SSR分子标记,利用21个已知萝卜染色体组成的甘蓝型油菜—萝卜附加系材料,确定了萝卜F染色体特异的2个SSR标记RM-59-293和Rs1SSR3694-220,应用两个标记对白菜‘559’与甘蓝型油菜—萝卜F染色体附加系FF回交的BC1进行鉴定,筛选出10个含有萝卜F染色体的BC1单株。利用萝卜基因组特异探针pURsN对该10个含有萝卜F染色体的BC1单株的基因组进行荧光原位杂交,结果显示10个BC1均得到1个杂交信号,含有1条萝卜F染色体。进一步对该10个BC1单株进行主要农艺性状鉴定,筛选出形态接近白菜亲本‘559’的BC1-15单株和形态偏向白菜类的BC1-8、BC1-16、BC1-31和BC1-35单株。

关键词: 白菜, 附加系, 萝卜F染色体, SSR分子标记, 荧光原位杂交

Abstract:

Referring to the SSR molecular markers of chromosome F linkage group in the chromosome-assigned sequence-tagged linkage map of radish,two SSR molecular markers RM-59-293 and Rs1SSR3694-220 which were specific for radish chromosome F were confirmed by means of the known radish-chromosome information in the genetic background of 21 materials. Both SSR markers were used to identify the alien radish chromosome F in the genetic background of BC1 being derived from the backcross of Brassica rapa‘559’and rape-radish chromosome F addition line FF. As a result,10 BC1 plants added with alien radish chromosome F were obtained. Furthermore,the genome of the selected 10 BC1 plants with radish chromosome F were analyzed to detect the exogenous radish chromosome by fluorescence in situ hybridization technology using the radish genomic probe pURsN. The result showed that all of the 10 BC1 had one hybridization signal,which indicated that each of the tested BC1 plants was added with an exogenous radish chromosome. The main agronomic traits of the 10 BC1 plants were further identified. As a result,BC1-15 with the morphology similar to Chinese cabbage parent‘559’and BC1-8,BC1-16,BC1-31 and BC1-35 with the morphology partial to Chinese cabbage were selected.

Key words: Brassica rapa, additional line, radish chromosome F, SSR molecular marker, fluorescent in situ hybridization

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