蔬菜细胞质雄性不育的育性恢复研究进展

doi:10.16420/j.issn.0513-353x.2020-0795

摘要/Abstract

摘要：

近些年来,在蔬菜中报道了多种类型的细胞质雄性不育系及其恢复系,并定位和克隆了多个恢复基因,开发了与恢复基因紧密连锁的标记应用于蔬菜育种。从蔬菜作物细胞质雄性不育(CMS)育性恢复系、恢复基因的定位和克隆、恢复基因连锁标记的开发、恢复系在育种中的应用等方面对近些年的研究进展进行了概述,并探讨了存在的问题及未来发展方向。

关键词: 蔬菜, 细胞质雄性不育, 恢复基因, 基因定位, 基因克隆, 分子标记

Abstract:

In recent years,various types of cytoplasmic male sterile(CMS)lines and their restorer lines have been reported in vegetables,and a number of restorer genes have been located and cloned,and the markers closely linked with restorer genes have been developed and used in vegetable breeding. Here,recent progress on CMS restorer lines were reviewed,as well as the mapping and cloning of restorer genes,the marker development of restorer genes,and the application of restorer lines in breeding of vegetables. The existing problems and the development trend in the future were discussed.

Key words: vegetables, cytoplasmic male sterility, restorer gene, gene mapping, gene cloning, molecular marker

中图分类号:

于海龙, 任文静, 方智远, 杨丽梅, 庄木, 吕红豪, 王勇, 季佳磊, 张扬勇. 蔬菜细胞质雄性不育的育性恢复研究进展[J]. 园艺学报, 2021, 48(5): 1031-1046.

YU Hailong, REN Wenjing, FANG Zhiyuan, YANG Limei, ZHUANG Mu, LÜ Honghao, WANG Yong, JI Jialei, ZHANG Yangyong. Advances on Fertility Restoration for Cytoplasmic Male Sterility in Vegetables[J]. Acta Horticulturae Sinica, 2021, 48(5): 1031-1046.

图/表 3

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选育单位 Breeding institution	细胞质不育系 CMS lines	恢复系 Restorer lines	用于配制的杂交品种 Hybrids produced by three-line system	参考文献 Reference
沈阳市农业科学院 Shenyang Academy of Agricultural Sciences	8021A,9911A,2013A,A02-7,A022	001-4-1,二斧头017-4-1,梅花椒099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5 001-4-1,Erfutou 017-4-1,Meihuajiao 099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5	沈研13,沈研14,沈研15,沈研20 Shenyan 13,Shenyan 14,Shenyan 15,Shenyan 20	杨世周和赵雪云,1984;杨凤梅等,2008;薛庆华等,2014
江苏省农业科学院蔬菜研究所 Institute of Vegetables,Jiangsu Academy of Agricultural Sciences	21A,8A,17A	LS₃,LS₂,LS₇,5-2R₁,5-2R₂,5-2R₃	苏椒3号A,碧玉,江蔬5号 Sujiao 3A,Biyu,Jiangshu 5	赵华仑等,1995;刘金兵等,2002,2004;王述彬等,2007
中国农业大学 China Agricultural University	8907A, S200243A	8965,8534,8318,S200244C	农大082 Nongda 082	沈火林等,1994
湖南省蔬菜研究所 Hunan Vegetable Research Institute	9704A,8214A,5901A,T01-55A,9202A	5904,8001,9701,	湘研14号,湘辣1号,湘辣2号 Xiangyan 14, Xiangla 1,Xiangla 2	邹学校等,2000,2008
广州蔬菜研究中心 Guangzhou Vegetable Research Center	21A,33A	52号,580号,17号	辣优2号,辣优8号,辣优9号 Layou 2,Layou 8,Layou 9	黄贞等,2006
中国农业科学院蔬菜花卉研究所 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences	77013A	Perennial		张宝玺等,2001; Wang et al.,2004
北京市农林科学院蔬菜研究中心 Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences	181A	98199	京辣2号 Jingla 2	耿三省等,2005

选育单位 Breeding institution	细胞质不育系 CMS lines	恢复系 Restorer lines	用于配制的杂交品种 Hybrids produced by three-line system	参考文献 Reference
沈阳市农业科学院 Shenyang Academy of Agricultural Sciences	8021A,9911A,2013A,A02-7,A022	001-4-1,二斧头017-4-1,梅花椒099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5 001-4-1,Erfutou 017-4-1,Meihuajiao 099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5	沈研13,沈研14,沈研15,沈研20 Shenyan 13,Shenyan 14,Shenyan 15,Shenyan 20	杨世周和赵雪云,1984;杨凤梅等,2008;薛庆华等,2014
江苏省农业科学院蔬菜研究所 Institute of Vegetables,Jiangsu Academy of Agricultural Sciences	21A,8A,17A	LS₃,LS₂,LS₇,5-2R₁,5-2R₂,5-2R₃	苏椒3号A,碧玉,江蔬5号 Sujiao 3A,Biyu,Jiangshu 5	赵华仑等,1995;刘金兵等,2002,2004;王述彬等,2007
中国农业大学 China Agricultural University	8907A, S200243A	8965,8534,8318,S200244C	农大082 Nongda 082	沈火林等,1994
湖南省蔬菜研究所 Hunan Vegetable Research Institute	9704A,8214A,5901A,T01-55A,9202A	5904,8001,9701,	湘研14号,湘辣1号,湘辣2号 Xiangyan 14, Xiangla 1,Xiangla 2	邹学校等,2000,2008
广州蔬菜研究中心 Guangzhou Vegetable Research Center	21A,33A	52号,580号,17号	辣优2号,辣优8号,辣优9号 Layou 2,Layou 8,Layou 9	黄贞等,2006
中国农业科学院蔬菜花卉研究所 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences	77013A	Perennial		张宝玺等,2001; Wang et al.,2004
北京市农林科学院蔬菜研究中心 Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences	181A	98199	京辣2号 Jingla 2	耿三省等,2005

CMS类型 CMS type	恢复基因 Rf locus	标记类型(标记名称) Marker type(Marker name)	参考文献 Reference
Ogura CMS	Rfo,Rfk,Rft, Rfob,Rfoc, RsRf3-1/RsRf3-2,RsRf3-4 ~ RsRf3-7	RAPD(OPH11₄₁₀)、AFLP(AFLP₁₉₀),同工酶标记 Isozyme markers(pgi-2),AFLP(R3、R15、R5),RAPD(OPC02₁₁₅₀、OPD02₁₀₀₀、OPF06₁₂₀₀、OPG02₇₀₀),RFLP-PCR(2F/1R),KASP(Rfo-SNP1),RAPD(OPA-14₆₀₀), SCAR(SCC06-1894),STS(STS-A14、STS-K17)	Delourme & Eber,1992;Delourme et al.,1994;Murayama et al.,1999,2002;Brown et al.,2003;Desloire et al.,2003;Wang et al.,2008,2013,2017;Yasumoto et al.,2009;张丽和王庆彪,2020
DCGMS	Rfd1	RAPD-PCR(R624、A137、A220)	Kim et al.,2010;Cho et al.,2012

CMS类型 CMS type	恢复基因 Rf locus	标记类型(标记名称) Marker type(Marker name)	参考文献 Reference
Ogura CMS	Rfo,Rfk,Rft, Rfob,Rfoc, RsRf3-1/RsRf3-2,RsRf3-4 ~ RsRf3-7	RAPD(OPH11₄₁₀)、AFLP(AFLP₁₉₀),同工酶标记 Isozyme markers(pgi-2),AFLP(R3、R15、R5),RAPD(OPC02₁₁₅₀、OPD02₁₀₀₀、OPF06₁₂₀₀、OPG02₇₀₀),RFLP-PCR(2F/1R),KASP(Rfo-SNP1),RAPD(OPA-14₆₀₀), SCAR(SCC06-1894),STS(STS-A14、STS-K17)	Delourme & Eber,1992;Delourme et al.,1994;Murayama et al.,1999,2002;Brown et al.,2003;Desloire et al.,2003;Wang et al.,2008,2013,2017;Yasumoto et al.,2009;张丽和王庆彪,2020
DCGMS	Rfd1	RAPD-PCR(R624、A137、A220)	Kim et al.,2010;Cho et al.,2012

标记名称 Marker name	标记类型 Marker type	遗传距离/cM Genetic distance	参考文献 Reference
OP13₁₄₀₀、OW19₈₀₀	rapd	0.37、8.12	Zhang et al.,2000
OPL09_-763	rapd	4.18	唐冬英等,2004
AFRF8-CAPS	aflp-caps	1.8	Kim et al.,2006
CRF3S1S	scar	4.8 ~ 5.3	Gulyas et al.,2006
E32M49-424bp、q18-1.0kb	aflp、rapd	3.1、2.2	周永坚,2007
PR-CAPS	caps	1.8	Lee et al.,2008a
CaRf-M1	rapd-pcr	1.1	Min et al.,2008
CRFCAPS、AFRF4/CaRf-FL-M	caps、aflp/sts	1.5、0.1/0.5	Min et al.,2009
BAC13T7SCAR、BAC17T7SCAR、PepPR1AS-PCR	scar	1.4、3.2、14	Jo et al.,2010
AF208834	ssr	20.8	杨娟等,2010
Co1Mod1-CAPS	caps	0	Jo et al.,2016
Capsicum-R-82CAPS	caps	0	林多等,2020
CaRf648	caps	0	Ortega et al.,2020