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园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 747-750.

• 研究简报 • 上一篇    下一篇

苹果新品种自交不亲和基因型的分子生物学鉴定

张雪梅1; 李保国1*; 崔惠英2; 齐国辉1
  

  1. (1 河北农业大学西校区林学院, 河北保定071000; 2 河北省林业技术推广总站, 石家庄050081)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

Molecular Biology Identif ication of Self-incompatibility Genotypes of NewApple Cultivars

ZHANG Xue-mei1; LI Bao-guo1*; CUI Hui-ying2; QI Guo-hui1
  

  1. (1 College of Forestry, Agricultural University of Hebei, Baoding, Hebei 071000, China; 2General Spreading Station of ForestryTechnique of Hebei Province, Shijiazhuang 050081,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要: 根据保守氨基酸序列FTQQYQ 和anti-1/M IWPNV 设计苹果自交不亲和基因引物P1 : 5′2 TT2
TACGCAGCAATATCAG23′; P2 : 5′2 ACGTTCGGCCAAATA /CATT23′, 利用PCR - RFLP分析方法, 以河北省
新引进的苹果品种美国8号、昂林、松本锦、华冠、南方脆幼叶为试验材料, 鉴定其自交不亲和基因型分
别为S9S27、S1S9、S9S 9、S2S9、S2S9

关键词: 苹果, 自交不亲和基因型, 分离, 鉴定

Abstract: Five new apple cultivars, USA No. 8, Korin, Matsumoto Nishiki, Huaguan and Nanfangcui,
which were introduced into Hebei Province recently, were used asmaterials in the p resent study. The p rimers
( P1 : 5′2 TTTACGCAGCAATATCAG23′; P2 : 5′2 ACGTTCGGCCAAATA /CATT23′) were newly designed ac2
cording to the conservedsequence of amino acid of S-RNase. The self2incompatibility alleles of the cultivars
were isolated and identified using PCR - RFLP analysis. Based on the results, the self2incompatibility geno
types of the cultivarswere identified as S9S27、S1S9、S9S 9、S2S9、S2S9, respectively.
Apple; Self-incompatibility genotype; Isolation; Identification

Key words: Apple, Self-incompatibility genotype, Isolation, Identification