金针菇过氧化氢酶基因FfCAT1和FfCAT2的表达分析

doi:10.16420/j.issn.0513-353x.2024-0744

园艺学报 ›› 2025, Vol. 52 ›› Issue (7): 1769-1779.doi: 10.16420/j.issn.0513-353x.2024-0744

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

金针菇过氧化氢酶基因FfCAT1和FfCAT2的表达分析

张拓²^,^*, 聂梦涵²^,^*, 刘媛媛²^,³, 段昕莲¹, 韩星¹, 谢宝贵²^,^**(), 严俊杰¹^,^**()

¹ 中国农业科学院都市农业研究所，国家成都农业科技中心，成都 610213
² 福建农林大学生命科学学院菌物研究中心，福州 350002
³ 北京市农林科学院植物保护研究所，北京 100080

收稿日期:2025-04-11 修回日期:2025-06-10 出版日期:2025-07-23 发布日期:2025-07-23
通讯作者:
**E-mail：yanjunjie@caas.cn
**E-mail：yanjunjie@caas.cn
作者简介:
*共同一作
基金资助:
国家自然科学基金青年科学基金项目(32102458); 四川省科技计划项目(2024ZYD0195); 中国农业科学院科技创新工程项目(34-IUA-06); 四川省育种联合攻关项目(2023YZ001)

Expression Analysis of the Catalase Genes FfCAT1 and FfCAT2 in Flammulina filiformis

ZHANG Tuo², NIE Menghan², LIU Yuanyuan²^,³, DUAN Xinlian¹, HAN Xing¹, XIE Baogui²^,^**(), and YAN Junjie¹^,^**()

¹ Chengdu Agricultural Science and Technology Center，Institute of Urban Agriculture，Chinese Academy of Agricultural Sciences，Chengdu 610213，China
² Mycological Research Center，College of Life Sciences，Fujian Agriculture and Forestry University，Fuzhou 350002，China
³ Institute of Plant Protection，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100080，China

Received:2025-04-11 Revised:2025-06-10 Published:2025-07-23 Online:2025-07-23

摘要/Abstract

摘要：

为了探究过氧化氢酶基因（CAT）在金针菇（Flammulina filiformis）子实体生长发育过程中的作用，基于全基因组数据鉴定了两个过氧化氢酶基因，分别命名为FfCAT1、FfCAT2。FfCAT1基因开放阅读框长1 557 bp，编码518个氨基酸。FfCAT2基因开放阅读框长1 692 bp，编码563个氨基酸。两个基因编码的蛋白均位于细胞质内，无跨膜结构域，但FfCAT1不具备信号肽，而FfCAT2在第18 ~ 19位氨基酸残基间具有信号肽。保守结构域及系统进化树分析显示，FfCAT1和FfCAT2均属于单功能过氧化氢酶。qRT-PCR的结果显示，FfCAT1在成熟期菌盖中高表达，且在菌盖弹射孢子阶段表达量显著上调，菌褶中的表达量显著高于菌肉组织；FfCAT2在伸长期菌盖中高表达，但在菌盖不同发育阶段的表达量差异较小，仅在直径3.3 cm的菌盖中显著下调；在直径0.9 cm菌盖的菌褶和菌肉组织中无显著差异。研究结果表明，两个FfCAT基因在菌盖生长发育过程发挥不同作用，FfCAT1作为主效基因参与菌盖的发育调控，且与菌褶上孢子的弹射相关。

关键词: 金针菇, 过氧化氢酶, 子实体发育, 表达分析, 有性繁殖

Abstract:

In order to explore the role of catalase gene（CAT）in the development of fruit body in Flammulina filiformis，two catalase genes were identified based on the whole-genome of F. filiformis，named FfCAT1 and FfCAT2，respectively. The length open reading frame of the FfCAT1 gene was 1 557 bp and encoded 518 amino acids. The length open reading frame of the FfCAT2 gene was 1 692 bp and encoded 563 amino acids. The proteins encoded by both genes were located in the cytoplasm，contained no transmembrane domains. However，FfCAT1 did not have a signal peptide，while FfCAT2 had signal peptide between amino acid residues 18-19. Conserved domain and phylogenetic tree analysis showed that FfCAT1 and FfCAT2 belonged to monofunctional catalases. The qRT-PCR results suggested that FfCAT1 was highly expressed in the pileus of maturation stage and the expression level was significantly up-regulated in the ejection spore stage of pileus. The expression level in the lamella was significantly higher than that in the context. FfCAT2 was highly expressed in the pileus of elongation stage，but the expression of FfCAT2 gene was less different in different stages of development of the pileus，and it was significantly down-regulated when the pileus diameter was 3.3 cm. There were no significant differences in the lamella and context of 0.9 cm diameter pileus. These results indicated that two FfCAT genes played different roles in the growth and development of the pileus. FfCAT1 was the major gene involved in the regulation of the development of the pileus and was related to the ejection of spores on the lamella.

Key words: Flammulina filiformis, catalase, fruit body development, expression analysis, sexual reproduction

张拓, 聂梦涵, 刘媛媛, 段昕莲, 韩星, 谢宝贵, 严俊杰. 金针菇过氧化氢酶基因FfCAT1和FfCAT2的表达分析[J]. 园艺学报, 2025, 52(7): 1769-1779.

ZHANG Tuo, NIE Menghan, LIU Yuanyuan, DUAN Xinlian, HAN Xing, XIE Baogui, and YAN Junjie. Expression Analysis of the Catalase Genes FfCAT1 and FfCAT2 in Flammulina filiformis[J]. Acta Horticulturae Sinica, 2025, 52(7): 1769-1779.

图/表 8

表1 本研究中所用的引物序列

Table 1 Primers sequence in this study

用途Function	引物名称Primer name	上游引物（5′-3′）Forward primer	下游引物（5′-3′）Reverse primer
PCR验证 PCR validation	FfCAT1	TCTTGACACCTTTCCTCGTAG	ACATTGCTTGCTTGTTTATTT
PCR验证 PCR validation	FfCAT2	GGTCCTCTCGTCGGTCGGCAATC	TTCCCACAGCATCCTCCGTCCTG
荧光定量PCR验证 qRT-PCR	QFfCAT1	CTCACTTTGACCGAGAGCGTAT	TGTTGGAGGTAATGTCGGAGAG
	QFfCAT2	AGCGCCCATATGTTGACGAA	TTTCCGAGATGACCAGCGAC
	QFfGAPDH	CCTCTGCTCACTTGAAGGGT	GCGTTGGAGATGACTTTGAA

图1 金针菇FfCAT1、FfCAT2基因结构示意图

Fig. 1 Schematic map of the FfCAT1 and FfCAT2 genes of Flammulina filiformis

图2 金针菇FfCAT蛋白的三级结构

Fig. 2 Tertiary structures of Flammulina filiformis FfCAT proteins

图3 基于邻近法（NJ）构建的三种类型过氧化氢酶系统进化树分支处数字代表自检置信度（Bootstrap = 1000）

Fig. 3 Phylogenetic tree of three types of catalase constructed based on the neighbor-joining method（NJ） The numbers at the branches represent the self-check confidence（Bootstrap = 1000）

图4 金针菇与其他担子菌单功能过氧化氢酶的氨基酸多序列比对与保守结构域分析下划线为保守结构域；倒三角为高度保守的近远端氨基酸残基

Fig. 4 Multiple sequence alignment and conserved domain analysis of monofunctional catalases amino acid sequences between Flammulina filiformis and other basidiomycetes Underlines denote conserved domains，and inverted triangles mark highly conserved proximal and distal amino acid residues

图5 金针菇FfCAT1及FfCAT2基因在不同子实体组织中的差异表达不同小写字母代表0.05水平差异显著

Fig. 5 The gene expression patterns of FfCAT1 and FfCAT2 during fruit body developments of Flammulina filiformis The different lowercase letters indicate significant differences at the 0.05 level

图6 金针菇Fv01菌盖发育过程形态特征（A）和FfCAT1及FfCAT2（B）基因在菌盖不同发育阶段的差异表达不同小写字母代表0.05水平差异显著

Fig. 6 Morphological characteristics of Fv01 pileus during development（A）and the gene expression patterns of FfCAT1 and FfCAT2（B）during pileus developments of Flammulina filiformis Fv01 The different lowercase letters indicate significant differences at the 0.05 level，n = 3

图7 金针菇FfCAT1及FfCAT2基因在直径0.9 cm菌盖不同组织中的差异表达

Fig. 7 The gene expression patterns of FfCAT1 and FfCAT2 in pileus of Flammulina filiformis ** P < 0.01，n = 3

参考文献 39

[1]	Bai Xiaoming, Dong Shiwei, Yang Yuning, Song Yue, Zhang Hanguo, Li Shujuan. 2019. Cloning and expression analysis of catalase gene(LoCAT1)from Larix olgensis. Bulletin of Botanical Research, 39 (4):539-546. (in Chinese) doi: 10.7525/j.issn.1673-5102.2019.04.008
	白晓明, 董实伟, 杨宇宁, 宋跃, 张含国, 李淑娟. 2019. 长白落叶松过氧化氢酶基因LoCAT1克隆及表达分析. 植物研究, 39 (4):539-546. doi: 10.7525/j.issn.1673-5102.2019.04.008
[2]	Černý M, Habánová H, Berka M, Luklová M, Brzobohatý B. 2018. Hydrogen peroxide:its role in plant biology and crosstalk with signalling networks. International Journal of Molecular Sciences, 19 (9):2812.
[3]	Díaz A, Loewen P C, Fita I, Carpena X. 2012. Thirty years of heme catalases structural biology. Archives of Biochemistry and Biophysics, 525 (2):102-110. doi: 10.1016/j.abb.2011.12.011 pmid: 22209752
[4]	Fan Liying, Fan Tingting, Tong Zongjun, Liang Liyun, Zhao Zhiyong, Chen Hui, Zhou Changyan, Zhao Xiaoyan. 2023. Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress. Acta Agriculturae Zhejiangensis, 35 (10):2321-2331. (in Chinese) doi: 10.3969/j.issn.1004-1524.20221550
	范丽莹, 范婷婷, 仝宗军, 梁立韵, 赵志勇, 陈辉, 周昌艳, 赵晓燕. 2023. 镉胁迫对不同品种羊肚菌镉富集规律与抗氧化系统的影响. 浙江农业学报, 35 (10):2321-2331. doi: 10.3969/j.issn.1004-1524.20221550
[5]	Feng Yujie. 2019. Study the degenerated mechanism of Cordyceps militaris strain and the key cultivation technology in solar greenhouse of Xinjiang[Ph. D. Dissertation]. Shihezi: Shihezi University. (in Chinese)
	冯玉杰. 2019. 蛹虫草退化机制及新疆温室关键栽培技术的研究[博士论文]. 石河子: 石河子大学.
[6]	Hu L F, Yang Y G, Jiang L W, Liu S Q. 2016. The catalase gene family in cucumber:genome-wide identification and organization. Genetics and Molecular Biology,39:408-415.
[7]	Kües U. 2000. Life history and developmental processes in the basidiomycete Coprinus cinereus. Microbiology and Molecular Biology Reviews, 64 (2):316-353. doi: 10.1128/MMBR.64.2.316-353.2000 pmid: 10839819
[8]	Liu Ao. 2024. Omics analysis of fruiting body development and changes of antioxidant activity in Flammulina filiformis[Ph. D. Dissertation]. Tai’an: Shandong Agricultural University. (in Chinese)
	刘澳. 2024. 金针菇子实体发育过程的组学分析和抗氧化活性变化[博士论文]. 泰安: 山东农业大学.
[9]	Liu Cong, Deng Yuhong, Liu Xuanming, Lin Jianzhong. 2023. Research advances in the function of catalase in plant growth,development and stress response. Life Science Research, 27 (2):128-138. (in Chinese)
	刘聪, 邓宇宏, 刘选明, 林建中. 2023. 过氧化氢酶在植物生长发育和胁迫响应中的功能研究进展. 生命科学研究, 27 (2):128-138.
[10]	Liu Qiyan, Qi Jun, Wang Zhuoren, Zhou Hongying, Sun Bo, Zhao Huizhang, Wu Hongli, Xu Zequn. 2021. Current situation and thinking of Flammulina filiformis in industrialized production in China. Edible Fungi of China, 40 (12):83-92. (in Chinese)
	刘启燕, 戚俊, 王卓仁, 周洪英, 孙波, 赵会长, 吴洪丽, 徐泽群. 2021. 我国金针菇工厂化生产现状与思考. 中国食用菌, 40 (12):83-92.
[11]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[12]	Lu Z, Kong X X, Lu Z M, Xiao M X, Chen M Y, Zhu L, Shen Y M, Hu X Y, Song S Y. 2014. Para-aminobenzoic acid(PABA)synthase enhances thermotolerance of mushroom Agaricus bisporus. PLoS ONE, 9 (3):e91298.
[13]	Meng Lijuan, Kong Weiwei, Wu Xiangli, Liu Xiuming, Huang Chenyang, Zhang Jinxia. 2015. Biochemical pathway analysis of exogenous NO improving heat‐tolerance of Pleurotus eryngii var. tuoliensis. Mycosystema, 34 (4):632-639. (in Chinese) doi: 10.13346/j.mycosystema.150038
	孟利娟, 孔维威, 邬向丽, 刘秀明, 黄晨阳, 张金霞. 2015. 外源一氧化氮提高白灵侧耳菌丝耐热性生化途径分析. 菌物学报, 34 (4):632-639. doi: 10.13346/j.mycosystema.150038
[14]	Mhamdi A, Queval G, Chaouch S, Vanderauwera S, Van Breusegem F, Noctor G. 2010. Catalase function in plants:a focus on Arabidopsis mutants as stress-mimic models. Journal of Experimental Botany, 61 (15):4197-4220. doi: 10.1093/jxb/erq282 pmid: 20876333
[15]	Qi Jingjing, Qin Xiujuan, Xie Yu, Chen Shanchun, He Yongrui, Li Qiang. 2021. Correlation analysis of citrus catalase gene CsKat01and citrus canker disease. Acta Horticulturae Sinica, 48 (1):26-36. (in Chinese) doi: 10.16420/j.issn.0513-353x.2020-0132
	祁静静, 秦秀娟, 谢宇, 陈善春, 何永睿, 李强. 2021. 过氧化氢酶基因CsKat01与柑橘溃疡病相关性分析. 园艺学报, 48 (1):26-36. doi: 10.16420/j.issn.0513-353x.2020-0132
[16]	Sakamoto Y. 2018. Influences of environmental factors on fruiting body induction,development and maturation in mushroom-forming fungi. Fungal Biology Reviews, 32 (4):236-248.
[17]	Sepasi T H, Moosavi-Movahedi A A. 2018. Catalase and its mysteries. Progress in Biophysics and Molecular Biology,140:5-12.
[18]	Sewelam N, Kazan K, Schenk P M. 2016. Global plant stress signaling:reactive oxygen species at the cross-road. Frontiers in Plant Science,7:187.
[19]	Stoyancheva G, Dishliyska V, Miteva Staleva J, Kostadinova J, Abrashev R, Angelova M, Krumova E. 2022. Sequencing and gene expression analysis of catalase genes in Antarctic fungal strain Penicillium griseofulvum P29. Polar Biology, 45 (3):437-447.
[20]	Swift M L. 1997. GraphPad prism,data analysis,and scientific graphing. Journal of Chemical Information and Computer Sciences, 37 (2):411-412.
[21]	Tao Y X, Chen R L, Yan J J, Long Y, Tong Z J, Song H B, Xie B G. 2019. A hydrophobin gene,Hyd9,plays an important role in the formation of aerial hyphae and primordia in Flammulina filiformis. Gene,706:84-90.
[22]	Tian Mimi. 2022. Analysis of the function and mechanism of catalase in the growth and development in Arabidopsis thaniala[Ph. D. Dissertation]. Hefei: Hefei University of Technology. (in Chinese)
	田蜜蜜. 2022. 过氧化氢酶在拟南芥生长发育中的功能及作用机制分析[博士论文]. 合肥: 合肥工业大学.
[23]	Tian Tian, Yao Lan, Fan Dongyu, Li Kexin, Li Changtian, Li Yu. 2021. Physiological and biochemical characteristics of degenerate mycelium of Flammulina filiformis. Microbiology China, 48 (10):3603-3611. (in Chinese)
	田甜, 姚澜, 范冬雨, 李可心, 李长田, 李玉. 2021. 金针菇退化菌丝的生理生化特征. 微生物学通报, 48 (10):3603-3611.
[24]	Wang L N, Wu X L, Gao W, Zhao M R, Zhang J X, Huang C Y. 2017. Differential expression patterns of Pleurotus ostreatus catalase genes during developmental stages and under heat stress. Genes(Basel), 8 (11):335.
[25]	Wang Lining. 2019. Characterization and function analysis of catalase genes in Pleurotus ostreatus[Ph. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	王丽宁. 2019. 糙皮侧耳过氧化氢酶基因特征分析和功能研究[博士论文]. 北京: 中国农业科学院.
[26]	Wang Lining, Huang Qinghua, Jiang Liqun, Ping Zhaohua. 2022a. Identification and expression analysis of catalase genes in Lepista sordida. Acta Edulis Fungi, 29 (5):12-21. (in Chinese)
	王丽宁, 黄清铧, 蒋丽群, 平兆华. 2022a. 花脸香蘑过氧化氢酶基因鉴定及表达分析. 食用菌学报, 29 (5):12-21.
[27]	Wang Lining, Huang Qinghua, Liang Lei, Wang Qingfu. 2022b. Identification and expression analysis of the catalase family genes in Funalia trogii. Mycosystema, 41 (8):1303-1313. (in Chinese)
	王丽宁, 黄清铧, 梁磊, 王庆福. 2022b. 硬毛粗盖孔菌过氧化氢酶家族基因鉴定及表达分析. 菌物学报, 41 (8):1303-1313.
[28]	Xia X J, Zhou Y H, Shi K, Zhou J, Foyer C H, Yu J Q. 2015. Interplay between reactive oxygen species and hormones in the control of plant development and stress tolerance. Journal of Experimental Botany, 66 (10):2839-2856.
[29]	Yan J J, Chekanova J, Liu Y Y, Gan B C, Long Y, Han X, Tong Z J, Miao J, Lian L D, Xie B G, Liu F. 2022. Reactive oxygen species distribution involved in stipe gradient elongation in the mushroom Flammulina filiformis. Cells, 11 (12):1896.
[30]	Yan J J, Tong Z J, Liu Y Y, Li Y N, Zhao C, Mukhtar I, Tao Y X, Chen B Z, Deng Y J, Xie B G. 2019. Comparative transcriptomics of Flammulina filiformis suggests a high CO₂ concentration inhibits early pileus expansion by decreasing cell division control pathways. International Journal of Molecular Sciences, 20 (23):5923.
[31]	Yan J J, Zhang L, Wang R Q, Xie B, Li X, Chen R L, Guo L X, Xie B G. 2016. The sequence characteristics and expression models reveal superoxide dismutase involved in cold response and fruiting body development in Volvariella volvacea. International Journal of Molecular Sciences, 17 (1):34.
[32]	Yang Huanling, Chang Tingting, Zhao Yan, You Huafang, Zha Lei, Li Zhengpeng, Tong Zongjun, Chen Mingjie. 2021. Expression of catalase gene(VvCAT1)from Volvariella volvacea in Escherichia coli and its temperature tolerance. Microbiology China, 48 (11):4054-4060. (in Chinese)
	杨焕玲, 常婷婷, 赵妍, 游华芳, 查磊, 李正鹏, 仝宗军, 陈明杰. 2021. 草菇过氧化氢酶基因(VvCAT1)异源表达及耐温度胁迫功能分析. 微生物学通报, 48 (11):4054-4060.
[33]	Yang Z, Mhamdi A, Noctor G. 2019. Analysis of catalase mutants underscores the essential role of CATALASE2 for plant growth and day length-dependent oxidative signalling. Plant Cell and Environment, 42 (2):688-700.
[34]	Yoshio K, Yoshioka Y, Toshikuni K. 2022. Physiological roles of catalases cat1 and cat2 in Myxococcus xanthus. Journal of Microbiology, 60 (12):1168-1177.
[35]	Yu Deling, Wang Changliu. 2016. Progresses in the research of catalase. Chinese Journal of Histochemistry and Cytochemistry, 25 (2):189-194. (in Chinese)
	于德玲, 王昌留. 2016. 过氧化氢酶的研究进展. 中国组织化学与细胞化学杂志, 25 (2):189-194.
[36]	Zamocky M, Furtmüller P G, Obinger C. 2008. Evolution of catalases from bacteria to humans. Antioxid Redox Signal, 10 (9):1527-1548.
[37]	Zhang Lei, Tong Zongjun, Yan Junjie, Huang Rongmei, Liu Yuanyuan, Li Bingyu, Wang Jianfang, Xie Baogui. 2018. Sequence characteristics and expression analysis of small G protein Ran in Flammulina filiformis. Acta Edulis Fungi, 25 (1):13-19. (in Chinese)
	张磊, 仝宗军, 严俊杰, 黄蓉梅, 刘媛媛, 李冰玉, 汪健芳, 谢宝贵. 2018. 金针菇小G蛋白Ran的序列特征与表达分析. 食用菌学报, 25 (1):13-19.
[38]	Zhu J N, Wang W P, Sun W H, Lei Y X, Tan Q F, Zhao G H, Yun J M, Zhao F Y. 2024. Overexpression of cat2 restores antioxidant properties and production traits in degenerated strains of Volvariella volvacea. Free Radical Biology and Medicine,215:94-105.
[39]	Zhu W W, Hu J B, Chi J L, Li Y, Yang B, Hu W L, Chen F, Xu C, Chai L S, Bao Y M. 2020. Label-free proteomics reveals the molecular mechanism of subculture induced strain degeneration and discovery of indicative index for degeneration in Pleurotus ostreatus. Molecules, 25 (21):4920.

金针菇过氧化氢酶基因FfCAT1和FfCAT2的表达分析

Expression Analysis of the Catalase Genes FfCAT1 and FfCAT2 in Flammulina filiformis

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金针菇过氧化氢酶基因FfCAT1和FfCAT2的表达分析

Expression Analysis of the Catalase Genes FfCAT1 and FfCAT2 in Flammulina filiformis

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