姜DUF966基因家族成员鉴定与表达分析

doi:10.16420/j.issn.0513-353x.2023-0422

摘要/Abstract

摘要：

为了解姜DUF966家族基因（ZoDUF966）的功能，利用生物学信息方法对其进行系统的鉴定。共鉴定出11个ZoDUF966，在系统发育上被划分为a、b和c组，分别包含5、4和2个成员。所有ZoDUF966蛋白均为亲水性蛋白并定位于细胞核；除ZoDUF966_1b、ZoDUF966_3b和ZoDUF966_11a外，其余8个均为碱性蛋白；蛋白质二级结构以α-螺旋和无规则卷曲为主，三级结构存在一定的差异。11个ZoDUF966分布在6条染色体上，6和16号染色体上分别有4和3个，还有4条染色体上均只有1个。ZoDUF966的启动子序列中含有大量与光响应、胁迫、生长发育以及植物激素相关的顺式作用元件。基于转录组测序数据的基因表达分析发现，ZoDUF966在不同生长时期、不同组织、生物胁迫以及非生物胁迫中的表达均存在差异，其中ZoDUF966_6c响应低温胁迫，ZoDUF966_9b则在接种腐皮镰刀菌后高度上调表达。qRT-PCR结果显示，ZoDUF966响应干旱、淹水和盐胁迫。干旱胁迫下，根系中ZoDUF966_6c/10a/11a和根茎中的ZoDUF966_7a/11a显著上调表达，根茎中ZoDUF966_1b/4b/8a/1a显著下调表达。盐胁迫下，根系中ZoDUF966_9b/7a显著上调表达，根系中ZoDUF966_5a显著下调表达。淹水胁迫下，根茎中ZoDUF966_7a和ZoDUF966_10a分别显著上调,叶中ZoDUF966_1b和根系中的ZoDUF966_11a显著下调表达。

关键词: 姜, DUF966, 生物信息学, 表达分析

Abstract:

In order to understand the function of DUF966 family genes（ZoDUF966）in ginger，the biological information method was used to systematically identify them. A total of 11 ZoDUF966 were identified and classified into groups a，b and c，including 5，4 and 2 members，respectively. All ZoDUF966 proteins were hydrophilic proteins and localized in the nucleus. Except for ZoDUF966_1b，ZoDUF966_3b and ZoDUF966_11a，the other 8 proteins were alkaline proteins. In the secondary structure of the protein，α-helix and random coil are dominant，and there are some differences in the tertiary structure. Eleven ZoDUF966 were distributed on six chromosomes，except for four and three genes on chromosomes 6 and 16，respectively，and only one gene on each of the other four chromosomes. The promoter sequence of ZoDUF966 contains cis-acting elements related to light response，stress，growth and development，and plant hormones. Gene expression analysis based on transcriptome sequencing data showed that the expression of ZoDUF966 was different in different growth periods，different tissues，biotic and abiotic stresses. Among them，ZoDUF966_6c responded to low temperature stress，and ZoDUF966_9b was highly up-regulated after inoculation with Fusarium solani. qRT-PCR results showed that ZoDUF966 responded to drought，flooding and salt stresses. Under drought stress，ZoDUF966_6c/10a/11a in roots and ZoDUF966_7a/11a in rhizomes were significantly up-regulated，while ZoDUF966_1b/4b and ZoDUF966_8a/1a in rhizomes were significantly down-regulated. Under salt stress，ZoDUF966_9b and ZoDUF966_7a were significantly up-regulated in roots，and ZoDUF966_5a was significantly down-regulated in roots. Under waterlogging stress，ZoDUF966_7a and ZoDUF966_10a were significantly up-regulated in rhizome，while ZoDUF966_1b in leaves and ZoDUF966_11a in roots were significantly down-regulated.

Key words: ginger, DUF966, bioinformatics, expression analysis

郭昌权, 李丹琪, 惠馨冉, 郑婧雅, 侯梦璐, 朱永兴. 姜DUF966基因家族成员鉴定与表达分析[J]. 园艺学报, 2024, 51(9): 2031-2047.

GUO Changquan, LI Danqi, HUI Xinran, ZHENG Jingya, HOU Menglu, ZHU Yongxing. Identification and Expression Pattern Analysis of DUF966 Gene Family Members in Ginger[J]. Acta Horticulturae Sinica, 2024, 51(9): 2031-2047.

图/表 12

表1 姜DUF966基因家族表达分析的实时荧光定量引物

Table 1 Real-time fluorescent quantitative primers for expression analysis of ginger DUF966 gene family

基因ID GeneID	名称 Name	引物序列（5′-3′） Primer sequence
Maker00000492	ZoDUF966_1b	F：AGACAGTGCGGCAGTTCCT；R：CCGCCAGGTCGGTCTTAT
Maker00034379	ZoDUF966_4b	F：CGGATTCTGGACTTCTACTTCTC；R：CAATGTCTTTGGCTTCTCGTC
Maker00034617	ZoDUF966_5a	F：TGAGCAGGCTCTGCGTTTG；R：TCGCCTTCCAGAACACTAAGAT
Maker00037034	ZoDUF966_6c	F：CCAGAACAGCGGAGCAATAA；R：GGTGGCAGTAGCGAGGGTT
Maker00037143	ZoDUF966_7.a	F：GCACCCGCACTTCATCGA；R：AATACAAGGCGGCCATCC
Maker00039356	ZoDUF966_8a	F：TGCCTTTGCGGCGGACTA；R：GCCTTGGGACGATTGCTC
Maker00055807	ZoDUF966_9b	F：TGTCGTCGTCGAGTTCGG；R：CCCGTGGTGGTCCTTCAT
Maker00065349	ZoDUF966_10a	F：ATCTTCGGTCGGGTTGGT；R：TGTACTCGTCCGGGTTCG
Maker00071931	ZoDUF966_11a	F：CCCGTCATCACCAGCAGA；R：CAACAACGCGGACATTCG
ACT	RBP	F：CCTATGAAGCGTAGAAACACAAG；R：GGAAGGACAACATCCCAAATC

图1 姜（Zo）、拟南芥（At）、黄瓜（Cs）和水稻（Os）DUF966成员的系统发育分析

Fig. 1 Phylogenetic analysis of DUF966 members from Zingiber officinale（Zo），Arabidopsis thaliana（At），Cucumis sativus（Cs）and Oryza sativa（Os）

表2 姜DUF966基因家族成员理化性质及二级结构

Table 2 Information and physicochemical properties of ZoDUF966 gene family members

命名 Name	基因ID Gene ID	长度/aa Length	分子量/kD Molecular weight	pI	不稳定指数 Instability index	亲水性Grand average of hydropathicity	α螺旋/% Alpha- helix	β折叠/% Beta- pleated sheet	无规则卷曲/% Random coil	延伸链/% Extended strand
ZoDUF966_1b	Maker00000492	276	29.97	6.61	55.20	-0.774	19.20	5.07	66.30	9.42
ZoDUF966_2c	Maker00001450	256	28.67	9.53	57.87	-0.980	25.78	9.38	55.08	9.77
ZoDUF966_3b	Maker00008957	539	59.07	6.18	64.98	-0.571	19.85	4.27	64.38	11.50
ZoDUF966_4b	Maker00034379	369	40.91	8.19	56.91	-0.696	21.68	6.50	59.08	12.74
ZoDUF966_5a	Maker00034617	461	50.76	8.17	62.65	-0.730	16.27	4.34	65.73	13.67
ZoDUF966_6c	Maker00037034	429	46.93	7.95	60.91	-0.719	26.57	4.43	58.28	10.72
ZoDUF966_7a	Maker00037143	455	50.99	8.54	60.85	-0.736	17.58	4.84	64.84	12.75
ZoDUF966_8a	Maker00039356	500	56.21	9.03	69.83	-0.775	18.45	4.96	61.71	14.88
ZoDUF966_9b	Maker00055807	413	45.97	8.83	48.50	-0.762	23.97	5.57	60.05	10.41
ZoDUF966_10a	Maker00065349	581	64.27	8.24	64.70	-0.733	17.90	3.79	66.27	12.05
ZoDUF966_11a	Maker00071931	442	48.89	6.39	72.23	-0.798	17.42	3.85	66.97	11.76

图2 ZoDUF966三级蛋白结构预测

Fig. 2 Prediction of protein tertiary structure of ZoDUF966

图3 ZoDUF966基因在染色体上的分布

Fig. 3 The distribution of ZoDUF966 gene on chromosome

表3 ZoDUF966重复基因对的Ka值和Ks值

Table 3 The Ka and Ks values of duplicated gene pairs of ZoDUF966

重复基因对 Duplicated gene pairs	异义替换 K_a	同义替换 K_s	K_a/K_s	选择类型 Types of selection
ZoDUF966_5a/ZoDUF966_10a	0.114	0.310	0.369	纯化选择 Purifying selection
ZoDUF966_8a/ZoDUF966_7a	0.122	0.407	0.301	纯化选择 Purifying selection
ZoDUF966_9b/ZoDUF966_1b	0.094	0.333	0.283	纯化选择 Purifying selection
ZoDUF966_10a/ZoDUF966_7a	0.310	0.982	0.316	纯化选择 Purifying selection

图4 ZoDUF966家族基因结构（A）和蛋白质保守基序（B）

Fig. 4 Analysis of gene structure（A）and conserved motif elements（B）of ZoDUF966 gene family

图5 ZoDUF966家族基因启动子顺式作用元件分布右侧色标表示启动子数量，色标最大值设定为5，数值超过或等于5的默认为一个颜色；方块中的数字代表每个基因在2 000 bp启动子区域包含的启动子数量。

Fig. 5 Putative cis-acting existed of ZoDUF966 gene family The right color label indicates the number of promoters. The maximum value of the color label is set to 5，and the default value of the value exceeding or equal to 5 is equal to 5；The number in each image block represents the number of promoters contained in the 2 000 bp promoter region of each gene.

图6 ZoDUF966在姜不同生育期各器官中的表达图中每个色标及其对应的数字表示不同的表达水平，颜色越深数字越大表示表达水平越高。

Fig. 6 ZoDUF966 expression in different organs during different reproductive stages of ginger Each color scale and its corresponding number in the figure represents a different level of expression. The darker the color，the larger the number，the higher the level of expression.

图7 姜ZoDUF966在不同贮藏温度（A）、接种青枯菌（B）、接种腐皮镰刀菌（C）处理下根茎中的表达以及在褪黑素（D）处理下叶中的表达图中每个色标及其对应的数字表示不同的表达水平，颜色越深数字数字越大表达水平越高。

Fig. 7 The expression of ZoDUF966 in the rhizome of ginger treated with different storage temperatures（A），inoculation with Ralstonia solanacearum（B），inoculation with Fusarium solani（C）and the expression in the leaves of ginger treated with melatonin（D） Each color scale and its corresponding number in the figure represents a different level of expression. The darker the color，the larger the number，the higher the level of expression.

图8 姜幼苗在干旱、淹水和盐胁迫处理下的表型特征

Fig. 8 Phenotypic characteristics of ginger seedlings under drought，flooding and salt stress

图9 姜幼苗在干旱、淹水和盐胁迫处理下不同组织中ZoDUF966的qRT-PCR表达量不同小写字母表示差异显著（P < 0.05）。

Fig. 9 qRT-PCR expression of ZoDUF966 in different tissue of ginger seedlings under drought，flooding and salt stresses Different lowercase letters indicate significantly different values（P < 0.05）.

参考文献 45

[1]	Bateman A, Coggill P, Finn R D. 2010. DUFs:families in search of function. Acta Crystallographica Section F,66:1148-1152.
[2]	Burkhardt A, Day B. 2016. Transcriptome and small RNAome dynamics during a resistant and susceptible interaction between cucumber and downy mildew. The Plant Genome, 9 (1):1-19.
[3]	Cao Yunpeng, Fang Zhi, Li Shumei, Yan Chongchong, Ding Qingqing, Cheng Xi, Lin Yi, Guo Ning, Cai Yongping. 2015. Genome wide identification and analyses of 4CL gene families in Pyrus bretschenideri Rehd. Hereditas, 37 (7):711-719. (in Chinese)
	曹运鹏, 方志, 李姝妹, 闫冲冲, 丁庆庆, 程曦, 林毅, 郭宁, 蔡永萍. 2015. 砀山酥梨4CL基因家族的全基因组鉴定与分析. 遗传, 37 (7):711-719.
[4]	Chen C J, Chen H, Zhang Y, Thomas H R, Frank M H, He Y H, Xia R. 2020. TBtools:an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13 (8):1194-1202.
[5]	Chen Juan. 2007. Effects of drought stress and exogenous ABA on ginger growth and rhizosphere effects[M. D. Dissertation]. Chengdu: Sichuan University. (in Chinese)
	陈娟. 2007. 干旱胁迫和外源ABA对生姜生长和根际效应的影响研究[硕士论文]. 成都: 四川大学.
[6]	Dong Y T, Li C, Zhang Y, He Q L, Daud M K, Chen J H, Zhu S J. 2016. Glutathione S-transferase gene family in Gossypium raimondii and G. arboretum:comparative genomic study and their expression under salt stress. Frontiers in Plant Science,7:193.
[7]	Guo C M, Luo C K, Guo L J, Li M, Guo X L, Zhang Y X, Wang L J, Chen L. 2016. OsSIDP366,a DUF 1644 gene,positively regulates responses to drought and salt stresses in rice. Journal of Integrative Plant Biology, 58 (5):492-502.
[8]	Huang M J, Xing H T, Li Z X, Li H L, Wu L, Jiang Y S. 2021a. Identification and expression profile of the soil moisture and Ralstonia solanacearum response CYPome in ginger(Zingiber officinale). Peer J,9:11755.
[9]	Huang W D, He Y Q, Yang L, Lu C, Zhu Y X, Sun C, Ma D F, Yin J L. 2021b. Genome-wide analysis of growth-regulating factors(GRFs)in Triticum aestivum. Peer J,9:10701.
[10]	Jiang Y S, Huang M J, Zhang M X, Lan J B, WangW X, Tao X, Liu Y Q. 2018. Transcriptome analysis provides novel insights into high-soil-moisture-elevated susceptibility to Ralstonia solanacearum infection in ginger(Zingiber officinale Roscoe cv. Southwest). Plant Physiology and Biochemistry,132:547-556.
[11]	Kim S J, Ryu M Y, Kim W T. 2012. Suppression of Arabidopsis RING-DUF 1117 E3 ubiquitin ligases,AtRDUF1 and AtRDUF2,reduces tolerance to ABA-mediated drought stress. Biochemical and Biophysical Research Communications, 420 (1):141-147.
[12]	Li G, Ma J W, Yin J L, Guo F L, Xi K Y, Yang P H, Cai X D, Jia Q, Li L, Liu Y Q, Zhu Y X. 2022a. Identification of reference genes for reverse transcription-quantitative PCR analysis of ginger under abiotic stress and for postharvest biology studies. Frontiers in Plant Science,13:1587.
[13]	Li H L, Wu L, Dong Z M, Jiang Y S, Jiang S J, Xing H T, Li Q, Liu G C, Tian S M, Wu Z Y, Bin W, Li Z X, Zhao P, Zhang Y, Tang J M, Xu J B, Huang K, Liu X, Zhang W L, Liao Q H, Ren Y, Huang X Z, Li Q Z, Li C Y, Wang Y, Xavier R B, Li H H, Liu Y, Wan T, Liu Q H, Zou Y, Jian J B, Xia Q Y, Liu Y W. 2021. Haplotype-resolved genome of diploid ginger(Zingiber officinale)and its unique gingerol biosynthetic pathway. Horticulture Research, 8 (1):242.
[14]	Li Qingqing, Li Dapeng, Li Dequan. 2010. The research progress in biosynthesis and regulation of jasmonates. Biotechnology Bulletin,(1):53-57,62. (in Chinese)
	李清清, 李大鹏, 李德全. 2010. 茉莉酸和茉莉酸甲酯生物合成及其调控机制. 生物技术通报,(1):53-57,62.
[15]	Li W C, Zhao S Q. 2012. Specific silencing of At1g13770 and At2g 23470 genes by artificial microRNAs in Arabidopsis thaliana. Hereditary, 34 (3):348-355.
[16]	Li Z Q, Wang C L, Wang K Y, Zhao J Y, Shao J R, Chen H, Zhou M L, Zhu X M. 2022b. Metal tolerance protein encoding gene family in Fagopyrum tartaricum:genome-wide identification,characterization and expression under multiple metal stresses. Plants, 11 (7):850.
[17]	Li Zhiqiang, Lie Shihui, Wang Xian, Xiang Li, Liu Enliang, Fang Furong. 2022. Genomen wide identification and bioinformatics of SOR family in barely. Plant Physiology Journal, 58 (8):1496-1506. (in Chinese)
	李志强, 聂石辉, 王仙, 向莉, 刘恩良, 方伏荣. 2022. 大麦SRO家族的全基因组鉴定及生物信息学分析. 植物生理学报, 58 (8):1496-1506.
[18]	Liu Longbo, Song Yunxian, Zhang Huijun, Yu Rugang, Zhu Qianzhi, Zheng Ke, Zheng Jie. 2021. Genome-wide identification and expression analysis of pomegranate GST gene family. Molecular Plant Breeding, 19 (16):5307-5317. (in Chinese)
	刘龙博, 宋运贤, 张慧君, 余如刚, 朱乾智, 郑珂, 郑洁. 2021. 石榴GST基因家族全基因组鉴定及表达分析. 分子植物育种, 19 (16):5307-5317.
[19]	Luo C K, Guo C M, Wang W J, Wang L J, Chen L. 2014. Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF 966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice. Plant Cell Reports, 33 (2):323-336.
[20]	Luo Chengke, Guo Chiming, Zhang Yuxia, Chen Jilin, Xiao Shimin, Chen Liang. 2013. Expression analysis a stress repressed gene OsRSR4 from DUF 966 family and generation of OsDSR4 overexpressing transgenic rice(Oryza sativa L. ssp. Japonica). Journal of Agricultural Biotechnology, 21 (11):1287-1294. (in Chinese)
	罗成科, 郭迟鸣, 张玉霞, 陈继林, 肖世民, 陈亮. 2013. DUF966家族中一个胁迫抑制基因OsDSR4的表达分析及超表达转基因水稻的获得. 农业生物技术学报, 21 (11):1287-1294.
[21]	Luo Chengke, Tian Lei. 2017. Bioinformatics analysis of rice DUF 966 gene family. Molecular Plant Breeding, 15 (12):4791-4796. (in Chinese)
	罗成科, 田蕾. 2017. 水稻DUF966基因家族的生物信息学分析. 分子植物育种, 15 (12):4791-4796.
[22]	Lynch M, Conery J S. 2000. The evolutionary fate and consequences of duplicate genes. Science, 290 (5494):1151-1155. doi: 10.1126/science.290.5494.1151 pmid: 11073452
[23]	Ma S F, Zhang Z Q, Long Y Q, Huo W Q, Zhang Y Z, Yang X Q, Zhang J, Li X Y, Du Q Y, Liu W, Yang D G, Ma X F. 2022. Evolutionary history and functional diversification of the JmjC domain-containing histone demethylase gene family in plants. Plants, 11 (8):1041.
[24]	Palmeros-Suárez P A, Massange-Sánchez J A, Sánchez-Segura L, Martínez-Gallardo N A, Espitia R E, Gómez-Leyva J F, Délano-Frier J P. 2017. AhDGR2,an amaranth abiotic stress-induced DUF 642 protein gene,modifies cell wall structure and composition and causes salt and ABA hyper-sensibility in transgenic Arabidopsis. Planta, 245 (3):623-640. doi: 10.1007/s00425-016-2635-y pmid: 27988887
[25]	Qin D D, Xie S, Liu G, Ni Z F, Yao Y Y, Sun Q X, Peng H R. 2013. Isolation and functional characterization of heat-stress responsive gene TaWTF1 from wheat. Chinese Bulletin of Botany,48:34-41.
[26]	Sharma H, Sharma A, Rajput R, Sidhu S, Dhillon H, Verma P C, Pandey A, Upadhyay S K. 2022. Molecular characterization,evolutionary analysis,and expression profiling of BOR genes in important cereals. Plants, 11 (7):911.
[27]	Shen L P, Zhong T, Wang L, Zhang Q Q, Jin H Y, Xu M L, Ye J R. 2019. Characterization the role of a UFC homolog,AtAuxRP3,in the regulation of Arabidopsis seedling growth and stress response. Journal of Plant Physiology,240:152990.
[28]	Tian J, Li Y, Hu Y, Zhong Q W, Yin J L, Zhu Y X. 2022. Mining the roles of cucumber DUF 966 genes in fruit development and stress response. Plants, 11 (19):2497.
[29]	Tirajoh A. 2005. Isolation and characterization of novel salt-responsive genes in tomato(Lycopersicon esculentum Mill.)roots. Simon Fraser University.
[30]	Thompson J D, Higgins D G, Gibson T J. 1994. CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22 (22):4673-4680. doi: 10.1093/nar/22.22.4673 pmid: 7984417
[31]	Varakumar S, Umesh K V, Singhal R S. 2017. Enhanced extraction of oleoresin from ginger(Zingiber officinale)rhizome powder using enzyme-assisted three phase partitioning. Food Chemistry,216:27-36.
[32]	Wang Lei, Xu Kun, Li Xiu. 2013. Research status and prospect of ginger germplasm resources and breeding. Chinese Vegetables,(16):1-6. (in Chinese)
	王磊, 徐坤, 李秀. 2013. 姜种质资源及育种研究现状与展望. 中国蔬菜,(16):1-6.
[33]	Wang Xiaojuan. 2014. Functional analysis of AtST39 and AtST39H in salt and drought stress[M. D. Dissertation]. Xiamen: Xiamen University. (in Chinese)
	王晓娟. 2014. AtST39和AtST39H在盐胁迫干旱胁迫中的功能研究[硕士论文]. 厦门: 厦门大学.
[34]	Xin Z, Mandaokar A, Chen J P, Last R, Browse J. 2007. Arabidopsis ESK1 encodes a novel regulator of freezing tolerance. The Plant Journal, 49 (5):786-799.
[35]	Xiong J Y, Chen D Y, Su T T, Shen Q F, Wu D Z, Zhang G P. 2022. Genome-wide identification,expression pattern and sequence variation analysis of SnRK family genes in barley. Plants, 11 (7):975.
[36]	Xu Yue, Chao Bili, Chen Zijing, Xu Kun. 2019. Effects of saline-alkali interaction stress on yield and quality of ginger and its mathematical model establishment. Chinese Vegetables,(12):41-45. (in Chinese)
	徐悦, 曹逼力, 陈子敬, 徐坤. 2019. 盐碱交互胁迫对生姜产量和品质的影响及其数学模型的建立. 中国蔬菜,(12):41-45.
[37]	Yang S Q, Li W Q, Miao H, Gan P F, Qiao L, Chang Y L, Shi C H, Chen K M. 2016. REL2,a gene encoding an unknown function protein which contains DUF630 and DUF 632 domains controls leaf rolling in rice. Rice, 9 (1):1-14.
[38]	Yang Y, Miao Y F, Zhong S W, Fang Q, Wang Y G, Dong B, Zhao H B. 2022. Genome-wide identification and expression analysis of XTH gene family during flower-opening stages in Osmanthus fragrans. Plants, 11 (8):1015.
[39]	Yin J L, Liu M Y, Ma D F, Wu J W, LI S L, Zhu Y X, Han B. 2018. Identification of circular RNAs and their targets during tomato fruit ripening. Postharvest Biology and Technology,136:90-98.
[40]	Zhang H M, Zhu J H, Gong Z Z, Zhu J K. 2022. Abiotic stress responses in plants. Nature Reviews Genetics, 23 (2):104-119.
[41]	Zhang P, Wang Y H, Wang J, Li Gang, Li S Y, Ma J W, Peng X Y, Yin J L, Liu Y Q, Zhu Y X. 2023. Transcriptomic and physiological analyses reveal changes in secondary metabolite and endogenous hormone in ginger(Zingiber officinale Rosc.)in response to postharvest chilling stress. Plant Physiology and Biochemistry,201:107799.
[42]	Zhang Y, Zhang F, Huang X Z. 2019. Characterization of an Arabidopsis thaliana DUF761-containing protein with a potential role in development and defense responses. Theoretical and Experimental Plant Physiology,31:303-316.
[43]	Zhao Shiyue, Ma Wenpei, Li Lin, Nie Hongfei, Ye Wenxiu, Li Guirong, Ji Wei. 2023. Identification and bioinformatics analysis of IDD gene family in Vitis vinifera. Molecular Plant Breeding, https://kns.cnki.net/kcms/detail/46.1068.S.20230328.1437.020.html. (in Chinese)
	赵士粤, 马文培, 黎霖, 聂鸿飞, 叶文秀, 李桂荣, 纪薇. 2023. 葡萄IDD基因家族鉴定及生物信息学分析. 分子植物育种, https://kns.cnki.net/kcms/detail/46.1068.S.20230328.1437.020.html.
[44]	Zhou X Y, Zhu X G, Shao W N, Song J H, Jiang W Q, He Q Y, Yin J L, Ma D F, Qiao Y L. 2020. Genome-wide mining of wheat DUF966 gene family provides new insights into salt stress responses. Frontiers in Plant Science,11:569838.
[45]	Zúñiga-Sánchez E, Soriano D, Martínez-Barajas E, Orozco-Segovia A, Gamboa-deBuen A. 2014. BIIDXI,the At4g32460 DUF 642 gene,is involved in pectin methyl esterase regulation during Arabidopsis thaliana seed germination and plant development. BMC Plant Biology, 14 (1):1-13.