番茄生物钟基因SlLNK1、SlEID1和SlELF3光周期响应分析

doi:10.16420/j.issn.0513-353x.2022-0627

园艺学报 ›› 2023, Vol. 50 ›› Issue (10): 2079-2090.doi: 10.16420/j.issn.0513-353x.2022-0627

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

番茄生物钟基因SlLNK1、SlEID1和SlELF3光周期响应分析

刘慧¹^,^*, 黄婷¹^,^*, 陶建平¹, 张佳琪¹, 张榕蓉¹, 宋刘霞³, 赵统敏³, 游雄²^,^**(), 熊爱生¹^,^**()

¹ 南京农业大学园艺学院，作物遗传与种质创新利用国家重点实验室，农业农村部华东地区园艺作物生物学与种质创制重点实验室，南京 210095
² 南京农业大学理学院，南京 210095
³ 江苏省农业科学院蔬菜研究所，南京 210014

收稿日期:2023-05-05 修回日期:2023-08-03 出版日期:2023-10-25 发布日期:2023-10-30
通讯作者: **（E-mail：xiongaisheng@njau.edu.cn，youx@njau.edu.cn）
作者简介:* 共同第一作者
基金资助:
江苏省自然科学基金青年基金项目(BK20221009); 中国博士后科学基金面上项目(2021M701742)

Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato

LIU Hui¹, HUANG Ting¹, TAO Jianping¹, ZHANG Jiaqi¹, ZHANG Rongrong¹, SONG Liuxia³, ZHAO Tongmin³, YOU Xiong²^,^**(), XIONG Aisheng¹^,^**()

¹ State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization，Key Laboratory of Horticultural Crop Biology and Germplasm Creation in East China of Ministry of Agriculture and Rural Affairs，College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China
² College of Sciences，Nanjing Agricultural University，Nanjing 210095，China
³ Institute of Vegetable Crops，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China

Received:2023-05-05 Revised:2023-08-03 Published:2023-10-25 Online:2023-10-30
Contact: **（E-mail：xiongaisheng@njau.edu.cn，youx@njau.edu.cn）

摘要/Abstract

摘要：

利用BLAST同源比对的方法鉴定获得番茄生物钟基因LNK1（night light-inducible and clock-regulated 1）、EID1（eia-like inhibitor of differentiation 1）和ELF3（early flowering 3）在35个物种中的直系同源基因，系统进化分析发现LNK1、EID1和ELF3起源于陆地植物。通过qRT-PCR证明三者均响应光周期和不同光照强度，光照显著诱导SlLNK1表达，抑制EID1和ELF3的表达。在2个光周期（48 h）中，番茄叶片净光合速率、胞间CO₂浓度、气孔导度和蒸腾速率呈节律变化。通过转录组分析3个基因的时空表达模式发现，它们在不同组织中呈现差异：SlLNK1在叶片、茎和花中高表达，SlEID1和SlELF3在根和果实中特异性表达。

关键词: 番茄, 生物钟基因, 光照强度, 表达分析, 光合特性

Abstract:

Circadian clock genes play important roles in the regulation of plant growth，development and metabolism. LNK1，EID1 and ELF3 are important circadian clock genes in tomato. In this study，orthologous genes of SlLNK1，SlEID1 and SlELF3 were identified by BLAST software in 35 species. The phylogenetic analysis found these genes were originated from land plants. qRT-PCR experiments showed that the three genes responded to photoperiod and different light intensities with different expression patterns. During two photoperiods（48 h），the net photosynthetic rate，intercellular CO₂ concentration，stomatal conductance，and transpiration rate changed rhythmically. Transcriptome analysis of temporal and spatial expression patterns of clock genes SlLNK1，SlEID1，and SlELF3 in tomato showed differences in different tissues.

Key words: tomato, circadian clock gene, light intensity, expression analysis, photosynthetic characteristics

刘慧, 黄婷, 陶建平, 张佳琪, 张榕蓉, 宋刘霞, 赵统敏, 游雄, 熊爱生. 番茄生物钟基因SlLNK1、SlEID1和SlELF3光周期响应分析[J]. 园艺学报, 2023, 50(10): 2079-2090.

LIU Hui, HUANG Ting, TAO Jianping, ZHANG Jiaqi, ZHANG Rongrong, SONG Liuxia, ZHAO Tongmin, YOU Xiong, XIONG Aisheng. Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato[J]. Acta Horticulturae Sinica, 2023, 50(10): 2079-2090.

图/表 10

参考文献 30

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基因名称	基因号	引物（5′-3′）
Gene name	Gene ID	Primer
SlLNK1	Solyc04g009050	F：TCAGACTACGGTGGCTGGTA；R：TCCCGTGCTGACACTTTCAA
SlEID1	Solyc09g075080	F：AGTTCGACGTCTTCGTGTCC；R：GCCAAATGTGAACAAGGCGA
SlELF3	Solyc11g070100	F：AGATTCTGAGTTGCACGCCA；R：AAAATGAGGCTGAGGCCGAA
Tubulin	Solyc04g077020	F：TGACGAAGTCAGGACAGGAA；R：CTGCATCTTCTTTGCCACTG

基因名称	基因号	引物（5′-3′）
Gene name	Gene ID	Primer
SlLNK1	Solyc04g009050	F：TCAGACTACGGTGGCTGGTA；R：TCCCGTGCTGACACTTTCAA
SlEID1	Solyc09g075080	F：AGTTCGACGTCTTCGTGTCC；R：GCCAAATGTGAACAAGGCGA
SlELF3	Solyc11g070100	F：AGATTCTGAGTTGCACGCCA；R：AAAATGAGGCTGAGGCCGAA
Tubulin	Solyc04g077020	F：TGACGAAGTCAGGACAGGAA；R：CTGCATCTTCTTTGCCACTG

物种 Species	数据来源 Data sources	物种 Species	数据来源
物种 Species	数据来源 Data sources	物种 Species	Data sources
脐形紫菜Porphyra umbilicalis	Phytozome	藜麦Chenopodium quinoa	Phytozome
莱茵衣藻Chlamydomonas reinhardtii	Phytozome	甜菜Beta vulgaris	Phytozome
小立碗藓Physcomitrium patens	Phytozome	辣椒Capsicum chinense	NCBI
江南卷柏Selaginella moellendorffii	Phytozome	茄子Solanum melongena	Phytozome
无油樟Amborella trichopoda	Phytozome	番茄Solanum lycopersicum	Phytozome
睡莲Nymphaea colorata	Phytozome	向日葵Helianthus annuus	Phytozome
凤梨Ananas comosus	Phytozome	莴苣Lactuca sativa	Phytozome
玉米Zea mays	Phytozome	三七Panax stipuleanatus	NCBI
水稻Oryza sativa	Phytozome	西洋参Panax quinquefolium	NCBI
黄连Coptis chinensis	NCBI	人参Panax ginseng	NCBI
耧斗菜Aquilegia coerulea	Phytozome	竹节参Panax japonicus	NCBI
葡萄Vitis vinifera	Phytozome	龙牙楤木Aralia elata	NCBI
长寿花Kalanchoe laxiflora	Phytozome	水芹Oenanthe sinensis	Unpublished
黄瓜Cucumis sativus	Phytozome	鸭儿芹Cryptotaenia japonica	Unpublished
森林草莓Fragaria vesca	Phytozome	香菜Coriandrum sativum	NCBI
拟南芥Arabidopsis thaliana	Phytozome	芹菜Apium graveolens	NCBI
萝卜Raphanus sativus	NCBI	欧芹Petroselinum crispum	Unpublished
白菜Brassica rapa	Phytozome	胡萝卜Daucus carota	NCBI

物种 Species	数据来源 Data sources	物种 Species	数据来源
物种 Species	数据来源 Data sources	物种 Species	Data sources
脐形紫菜Porphyra umbilicalis	Phytozome	藜麦Chenopodium quinoa	Phytozome
莱茵衣藻Chlamydomonas reinhardtii	Phytozome	甜菜Beta vulgaris	Phytozome
小立碗藓Physcomitrium patens	Phytozome	辣椒Capsicum chinense	NCBI
江南卷柏Selaginella moellendorffii	Phytozome	茄子Solanum melongena	Phytozome
无油樟Amborella trichopoda	Phytozome	番茄Solanum lycopersicum	Phytozome
睡莲Nymphaea colorata	Phytozome	向日葵Helianthus annuus	Phytozome
凤梨Ananas comosus	Phytozome	莴苣Lactuca sativa	Phytozome
玉米Zea mays	Phytozome	三七Panax stipuleanatus	NCBI
水稻Oryza sativa	Phytozome	西洋参Panax quinquefolium	NCBI
黄连Coptis chinensis	NCBI	人参Panax ginseng	NCBI
耧斗菜Aquilegia coerulea	Phytozome	竹节参Panax japonicus	NCBI
葡萄Vitis vinifera	Phytozome	龙牙楤木Aralia elata	NCBI
长寿花Kalanchoe laxiflora	Phytozome	水芹Oenanthe sinensis	Unpublished
黄瓜Cucumis sativus	Phytozome	鸭儿芹Cryptotaenia japonica	Unpublished
森林草莓Fragaria vesca	Phytozome	香菜Coriandrum sativum	NCBI
拟南芥Arabidopsis thaliana	Phytozome	芹菜Apium graveolens	NCBI
萝卜Raphanus sativus	NCBI	欧芹Petroselinum crispum	Unpublished
白菜Brassica rapa	Phytozome	胡萝卜Daucus carota	NCBI

番茄生物钟基因SlLNK1、SlEID1和SlELF3光周期响应分析

Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato

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番茄生物钟基因SlLNK1、SlEID1和SlELF3光周期响应分析

Analysis of Circadian Clock Genes SlLNK1，SlEID1，and SlELF3 Response to Photoperiod in Tomato

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