普通丝瓜Expansin家族基因的鉴定及其在果实膨大中的表达分析

doi:10.16420/j.issn.0513-353x.2024-0373

摘要/Abstract

摘要：

为揭示普通丝瓜（Luffa cylindrica）扩展蛋白（Expansin）家族基因的组成、结构和进化过程以及在果实膨大中的作用，利用生物信息技术从普通丝瓜基因组中鉴定出50个Expansin基因，分属于EXPA（18个）、EXPB（3个）、EXLA（27个）和EXLB（2个）4个亚家族，其中EXLA亚家族发生4对基因串联复制和6对基因片段复制，导致该亚家族基因数量明显扩增。系统发育树显示，4个亚家族成员各自聚集成群，且与同科的黄瓜亲缘关系较近。基因结构和保守基序分析显示，4个亚家族的外显子—内含子结构和保守基序种类及分布在进化上趋于保守，可以作为亚家族分型的重要依据。启动子元件预测分析发现，Expansin基因启动子中含有大量的光诱导、厌氧诱导、茉莉酸甲酯和脱落酸元件，而数量最多的光诱导元件只在EXLA/EXLB亚家族预测到。基于RNA-seq数据筛选出8个与果实横纵径发育极显著正相关的Expansin基因，其中4个已报道的基因LcEXPA8、LcEXPA18、LcEXPA14、LcEXPA1可作为丝瓜果形控制的潜在候选基因。对另外4个基因进行RT-qPCR分析，LcEXPA15在花后4 d显著高表达，LcEXPA3、LcEXPA16和LcEXPA7在花后8 ~ 15 d显著高表达。花后2 ~ 15 d是普通丝瓜的果实膨大期，这时期的果实细胞数量增加、单细胞明显膨大、伸长。由此推测，4个Expansin可能在催化细胞生长，促进果实膨大过程中发挥重要作用。通过构建8个Expansin互作网络，提示部分Expansin可能与ERF、bHLH、TCP和GATA这4类转录因子家族存在互作。以上8个Expansin基因可作为普通丝瓜果实膨大相关候选基因进行深入研究。

关键词: 普通丝瓜, Expansin, 基因家族, 果实膨大

Abstract:

To reveal the composition，structure，and evolutionary process of the Expansin family genes in Luffa cylindrica，as well as their role in fruit enlargement. This study identified 50 Expansin genes from the genome of sponge gourd by bioinformatics technology，which belong to 4 subfamilies[EXPA（18），EXPB（3），EXLA（27）and EXLB（2）]. Among them，the EXLA subfamily underwent 4 pairs of gene tandem duplication and 6 pairs of gene fragment duplication，resulting in a significant increase in the gene quantity in this subfamily. The phylogenetic tree showed that members of the four subfamilies gather in groups and were closely related to cucumbers. Gene structure and motif analysis showed that the exon intron structure and motif types and distributions of the four subfamilies tended to be conserved in evolution，which can serve as important basis for subfamily typing. Promoter element prediction analysis revealed that the Expansin gene promoter contained a large number of light inducible，anaerobic inducible，methyl jasmonate，and abscisic acid elements，with the highest number of light inducible elements predicted only in the EXLA/EXLB subfamily. Based on RNA-seq data，8 Expansin genes were screened，which were significantly positively correlated with fruit transverse and longitudinal diameter development. Among them，four genes LcEXPA8，LcEXPA18，LcEXPA14，and LcEXPA1 have been reported as potential candidate genes for controlling the fruit shape of sponge gourd. RT-qPCR analysis was performed on the other four genes，and LcEXPA15 was significantly overexpressed at 4 DAA（days after anthesis），LcEXPA3，LcEXPA16 and LcEXPA7 were significantly overexpressed at 8-15 DAA. It was known that 2-15 DAA was the fruit enlargement stage in sponge gourd，during which the number of fruit cells increased and single cells significantly expanded and elongated. So it was concluded that the 4 Expansin genes may play an important role in catalyzing cell growth and promoting fruit enlargement. By constructing 8 Expansin genes interaction network，it was suggested that some Expansin genes may interact with ERF，bHLH，TCP，and GATA four transcription factor families. The above 8 Expansin genes could be selected as candidate genes related with fruit enlargement in sponge gourd for further study.

Key words: Luffa cylindrica, Expansin, gene family, fruit enlargement

陈敏氡, 王彬, 白昌辉, 裘波音, 刘建汀, 温庆放, 朱海生, 李永平. 普通丝瓜Expansin家族基因的鉴定及其在果实膨大中的表达分析[J]. 园艺学报, 2025, 52(6): 1488-1504.

CHEN Mindong, WANG Bin, BAI Changhui, QIU Boyin, LIU Jianting, WEN Qingfang, ZHU Haisheng, and LI Yongping. Identification of Expansin Family Gene and Its Expression Analysis During Fruit Enlargement in Sponge Gourd[J]. Acta Horticulturae Sinica, 2025, 52(6): 1488-1504.

图/表 11

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基因Gene	引物序列Primer sequence（5′-3′）
LcEXPA16	F：AAACGGAAATAGTAGCGG；R：TCCAATCAGCAGGGAGTA
LcEXPA3	F：CCCAAGTGGTGTCTTCCTG；R：GCACGGTACTGAGCGATT
LcEXPA7	F：AGAAAGGTGGAGTGAGGTTCA；R：TATCGACACGGCATGGAC
LcEXPA15	F：CTCCAGTAGCCTCCCATTT；R：GCCATAACCTGCCTTGACT
18sRNA	F：GCTTGGGTGCTCGACAAACT；R：TCCACAGAGCAATGTCAATGG

基因Gene	引物序列Primer sequence（5′-3′）
LcEXPA16	F：AAACGGAAATAGTAGCGG；R：TCCAATCAGCAGGGAGTA
LcEXPA3	F：CCCAAGTGGTGTCTTCCTG；R：GCACGGTACTGAGCGATT
LcEXPA7	F：AGAAAGGTGGAGTGAGGTTCA；R：TATCGACACGGCATGGAC
LcEXPA15	F：CTCCAGTAGCCTCCCATTT；R：GCCATAACCTGCCTTGACT
18sRNA	F：GCTTGGGTGCTCGACAAACT；R：TCCACAGAGCAATGTCAATGG

基因名称 Gene name	基因ID Gene ID	染色体位置 Chromosome position		氨基酸/aa Amino acid number		分子量/kD Molecular weight		理论等电点 Theory isoelectric point
LcEXPA1	Maker00039481	Scaffold10：44274633..44276318（-）		242		59.74		5.12
LcEXPA2	Maker00039350	Scaffold10：42862024..42863052（-）		271		67.93		5.07
LcEXPA3	Maker00039137	Scaffold10：45546872..45547695（+）		247		61.82		5.08
LcEXPA4	Maker00036366	Scaffold5：48146177..48147973（+）		242		59.97		5.14
LcEXPA5	Maker00033676	Scaffold13：37888029..37890899（-）		258		63.83		5.10
LcEXPA6	Maker00030282	Scaffold6：14070765..14072443（+）		261		64.67		5.11
LcEXPA7	Maker00028836	Scaffold7：11466255..11470101（-）		252		64.36		5.05
LcEXPA8	Maker00026031	Scaffold7：2531229..2532764（-）		266		66.01		5.10
LcEXPA9	Maker00018301	Scaffold3：15686323..15688378（-）		264		65.19		5.10
LcEXPA10	Maker00016100	Scaffold6：42665910..42667187（+）		279		69.54		5.08
LcEXPA11	Maker00013380	Scaffold1：12581253..12583334（-）		289		71.29		5.07
LcEXPA12	Maker00012399	Scaffold1：13900544..13901668（+）		260		65.89		5.05
LcEXPA13	Maker00011031	Scaffold4：27627243..27635585（-）		510		129.04		4.94
LcEXPA14	Maker00009740	Scaffold7：8940229..8942250（-）		264		66.11		5.06
LcEXPA15	Maker00008644	Scaffold10：33380501..33385747（-）		338		84.60		5.05
LcEXPA16	Maker00006224	Scaffold4：5275140..5276227（-）		252		61.61		5.16
LcEXPA17	Maker00003094	Scaffold12：37764073..37766325（-）		287		71.50		5.08
LcEXPA18	Maker00000768	Scaffold8：44424275..44425647（+）		259		64.59		5.08
LcEXPB1	Maker00000264	Scaffold8：41943956..41945654（-）		353		87.82		5.03
LcEXPB2	Maker00031725	Scaffold11：4460008..：44601243（+）		274		68.75		5.07
LcEXPB3	Maker00008065	Scaffold4：7569358..7572344（-）		276		68.08		5.10
LcEXLA1	Maker00037537	Scaffold11：32691879..32693098（+）		259		63.88		5.17
LcEXLA2	Maker00037507	Scaffold11：32677443..32678122（+）		137		33.94		5.28
LcEXLA3	Maker00022629	Scaffold2：14547662..14548896（-）		264		65.08		5.18
LcEXLA4	Maker00022507	Scaffold2：14624877..14626093（+）		264		64.94		5.17
LcEXLA5	Maker00022429	Scaffold2：14529590..14530833（-）		259		63.86		5.18
LcEXLA6	Maker00022378	Scaffold2：14666085..14667284（+）		265		65.59		5.16
LcEXLA7	Maker00020500	Scaffold1：36285431..36288306（+）		474		119.30		4.99
LcEXLA8	Maker00015327	Scaffold3：29504343..29505828（-）		305		75.57		5.13
LcEXLA9	Maker00015140	Scaffold3：30071104..30076372（-）		264		64.55		5.13
LcEXLA10	Maker00015111	Scaffold3：29507534..29508742（-）		265		65.23		5.18
LcEXLA11	Maker00015029	Scaffold3：29059681..29062050（-）		356		87.97		5.07
LcEXLA12	Maker00015000	Scaffold3：29055967..29057177（-）		266		65.49		5.17
LcEXLA13	Maker00014988	Scaffold3：29376358..29377434（+）		263		65.19		5.17
LcEXLA14	Maker00014757	Scaffold3：29145720..29146827（-）		263		64.64		5.18
LcEXLA15	Maker00014734	Scaffold3：28994980..28996188（-）		265		64.84		5.18
LcEXLA16	Maker00014637	Scaffold3：28901855..28903092（-）		267		65.25		5.14
LcEXLA17	Maker00014364	Scaffold3：29435506..29437459（-）		266		65.41		5.16
LcEXLA18	Maker00014148	Scaffold3：30094458..30096603（-）		286		71.17		5.14
LcEXLA19	Maker00013924	Scaffold3：28963173..28964068（-）		235		57.10		5.21
LcEXLA20	Maker00013850	Scaffold3：29069173..29075111（-）		414		104.23		5.05
LcEXLA21	Maker00013746	Scaffold3：29194590..29195803（-）		247		60.62		5.20
LcEXLA22	Maker00013718	Scaffold3：29455193..29456551（-）		263		64.71		5.17
LcEXLA23	Maker00013618	Scaffold3：29098873..29100084（-）		269		66.51		5.15
LcEXLA24	Maker00005227	Scaffold11：33830538..33832189（+）		264		65.00		5.17
LcEXLA25	Maker00005193	Scaffold11：33797689..33799277（-）		298		74.46		5.13
LcEXLA26	Maker00005141	Scaffold11：33820779..33822086（-）		264		64.93		5.17
LcEXLA27	Maker00003985	Scaffold4：1360141..1361912（+）		198		49.36		5.22
LcEXLB1	Maker00038210	Scaffold12：39908175..39910085（-）		253		62.53		5.14
LcEXLB2	Maker00038088	Scaffold12：40370339..40373048（+）		256		62.82		5.15
基因名称 Gene name	基因ID Gene ID	蛋白不稳定指数 Protein instability index	脂溶指数 Lipid solubility index		疏水性 Total average hydrophobicity		亚细胞定位 Subcellular localization
LcEXPA1	Maker00039481	50.82	22.18		0.72		细胞外Extracellular
LcEXPA2	Maker00039350	51.01	22.26		0.79		细胞外Extracellular
LcEXPA3	Maker00039137	57.17	21.32		0.84		细胞外Extracellular
LcEXPA4	Maker00036366	41.14	21.35		0.62		内质网Endoplasmic
LcEXPA5	Maker00033676	48.80	24.42		0.80		细胞外Extracellular
LcEXPA6	Maker00030282	45.04	21.71		0.66		细胞外Extracellular
LcEXPA7	Maker00028836	61.26	19.97		0.92		叶绿体Chloroplast
LcEXPA8	Maker00026031	56.01	23.06		0.74		内质网Endoplasmic
LcEXPA9	Maker00018301	52.41	23.74		0.76		叶绿体Chloroplast
LcEXPA10	Maker00016100	53.80	24.25		0.77		叶绿体Chloroplast
LcEXPA11	Maker00013380	41.43	23.76		0.82		叶绿体Chloroplast
LcEXPA12	Maker00012399	56.19	18.85		0.85		细胞外Extracellular
LcEXPA13	Maker00011031	48.98	21.83		0.83		液泡Vacuole
LcEXPA14	Maker00009740	52.76	21.34		0.86		细胞外Extracellular
LcEXPA15	Maker00008644	48.99	21.79		0.71		叶绿体Chloroplast
LcEXPA16	Maker00006224	43.52	27.25		0.65		内质网Endoplasmic
LcEXPA17	Maker00003094	48.54	24.62		0.80		细胞质Cytoplasm
LcEXPA18	Maker00000768	50.04	21.24		0.81		细胞外Extracellular
LcEXPB1	Maker00000264	48.95	23.23		0.76		叶绿体Chloroplast
LcEXPB2	Maker00031725	50.10	20.07		0.74		细胞外Extracellular
LcEXPB3	Maker00008065	43.53	22.34		0.68		液泡Vacuole
LcEXLA1	Maker00037537	42.07	30.76		0.70		叶绿体Chloroplast
LcEXLA2	Maker00037507	42.46	27.01		0.61		叶绿体Chloroplast
LcEXLA3	Maker00022629	41.60	32.20		0.69		叶绿体Chloroplast
LcEXLA4	Maker00022507	40.72	32.45		0.72		细胞外Extracellular
LcEXLA5	Maker00022429	38.76	32.43		0.68		叶绿体Chloroplast
LcEXLA6	Maker00022378	36.96	32.20		0.72		叶绿体Chloroplast
LcEXLA7	Maker00020500	51.72	24.40		0.74		叶绿体Chloroplast
LcEXLA8	Maker00015327	41.21	30.27		0.71		叶绿体Chloroplast
LcEXLA9	Maker00015140	37.36	26.39		0.71		细胞外Extracellular
LcEXLA10	Maker00015111	38.05	29.94		0.64		细胞外Extracellular
LcEXLA11	Maker00015029	47.43	27.15		0.71		细胞质Cytoplasm
LcEXLA12	Maker00015000	43.60	30.08		0.66		细胞外Extracellular
LcEXLA13	Maker00014988	38.12	27.63		0.60		细胞外Extracellular
LcEXLA14	Maker00014757	39.28	29.66		0.61		叶绿体Chloroplast
LcEXLA15	Maker00014734	37.80	30.06		0.63		叶绿体Chloroplast
LcEXLA16	Maker00014637	39.87	25.97		0.66		细胞外Extracellular
LcEXLA17	Maker00014364	42.27	31.70		0.72		细胞外Extracellular
LcEXLA18	Maker00014148	39.96	29.49		0.70		细胞外Extracellular
LcEXLA19	Maker00013924	33.66	30.64		0.62		叶绿体Chloroplast
LcEXLA20	Maker00013850	41.44	26.17		0.67		内质网Endoplasmic
LcEXLA21	Maker00013746	37.90	30.90		0.62		叶绿体Chloroplast
LcEXLA22	Maker00013718	42.64	30.42		0.68		细胞外Extracellular
LcEXLA23	Maker00013618	42.52	30.61		0.70		叶绿体Chloroplast
LcEXLA24	Maker00005227	39.20	30.18		0.67		细胞外Extracellular
LcEXLA25	Maker00005193	47.09	31.10		0.72		叶绿体Chloroplast
LcEXLA26	Maker00005141	34.91	30.43		0.67		细胞外Extracellular
LcEXLA27	Maker00003985	43.48	29.12		0.64		液泡Vacuole
LcEXLB1	Maker00038210	41.61	26.48		0.68		细胞外Extracellular
LcEXLB2	Maker00038088	37.14	26.69		0.66		细胞外Extracellular

基因名称 Gene name	基因ID Gene ID	染色体位置 Chromosome position		氨基酸/aa Amino acid number		分子量/kD Molecular weight		理论等电点 Theory isoelectric point
LcEXPA1	Maker00039481	Scaffold10：44274633..44276318（-）		242		59.74		5.12
LcEXPA2	Maker00039350	Scaffold10：42862024..42863052（-）		271		67.93		5.07
LcEXPA3	Maker00039137	Scaffold10：45546872..45547695（+）		247		61.82		5.08
LcEXPA4	Maker00036366	Scaffold5：48146177..48147973（+）		242		59.97		5.14
LcEXPA5	Maker00033676	Scaffold13：37888029..37890899（-）		258		63.83		5.10
LcEXPA6	Maker00030282	Scaffold6：14070765..14072443（+）		261		64.67		5.11
LcEXPA7	Maker00028836	Scaffold7：11466255..11470101（-）		252		64.36		5.05
LcEXPA8	Maker00026031	Scaffold7：2531229..2532764（-）		266		66.01		5.10
LcEXPA9	Maker00018301	Scaffold3：15686323..15688378（-）		264		65.19		5.10
LcEXPA10	Maker00016100	Scaffold6：42665910..42667187（+）		279		69.54		5.08
LcEXPA11	Maker00013380	Scaffold1：12581253..12583334（-）		289		71.29		5.07
LcEXPA12	Maker00012399	Scaffold1：13900544..13901668（+）		260		65.89		5.05
LcEXPA13	Maker00011031	Scaffold4：27627243..27635585（-）		510		129.04		4.94
LcEXPA14	Maker00009740	Scaffold7：8940229..8942250（-）		264		66.11		5.06
LcEXPA15	Maker00008644	Scaffold10：33380501..33385747（-）		338		84.60		5.05
LcEXPA16	Maker00006224	Scaffold4：5275140..5276227（-）		252		61.61		5.16
LcEXPA17	Maker00003094	Scaffold12：37764073..37766325（-）		287		71.50		5.08
LcEXPA18	Maker00000768	Scaffold8：44424275..44425647（+）		259		64.59		5.08
LcEXPB1	Maker00000264	Scaffold8：41943956..41945654（-）		353		87.82		5.03
LcEXPB2	Maker00031725	Scaffold11：4460008..：44601243（+）		274		68.75		5.07
LcEXPB3	Maker00008065	Scaffold4：7569358..7572344（-）		276		68.08		5.10
LcEXLA1	Maker00037537	Scaffold11：32691879..32693098（+）		259		63.88		5.17
LcEXLA2	Maker00037507	Scaffold11：32677443..32678122（+）		137		33.94		5.28
LcEXLA3	Maker00022629	Scaffold2：14547662..14548896（-）		264		65.08		5.18
LcEXLA4	Maker00022507	Scaffold2：14624877..14626093（+）		264		64.94		5.17
LcEXLA5	Maker00022429	Scaffold2：14529590..14530833（-）		259		63.86		5.18
LcEXLA6	Maker00022378	Scaffold2：14666085..14667284（+）		265		65.59		5.16
LcEXLA7	Maker00020500	Scaffold1：36285431..36288306（+）		474		119.30		4.99
LcEXLA8	Maker00015327	Scaffold3：29504343..29505828（-）		305		75.57		5.13
LcEXLA9	Maker00015140	Scaffold3：30071104..30076372（-）		264		64.55		5.13
LcEXLA10	Maker00015111	Scaffold3：29507534..29508742（-）		265		65.23		5.18
LcEXLA11	Maker00015029	Scaffold3：29059681..29062050（-）		356		87.97		5.07
LcEXLA12	Maker00015000	Scaffold3：29055967..29057177（-）		266		65.49		5.17
LcEXLA13	Maker00014988	Scaffold3：29376358..29377434（+）		263		65.19		5.17
LcEXLA14	Maker00014757	Scaffold3：29145720..29146827（-）		263		64.64		5.18
LcEXLA15	Maker00014734	Scaffold3：28994980..28996188（-）		265		64.84		5.18
LcEXLA16	Maker00014637	Scaffold3：28901855..28903092（-）		267		65.25		5.14
LcEXLA17	Maker00014364	Scaffold3：29435506..29437459（-）		266		65.41		5.16
LcEXLA18	Maker00014148	Scaffold3：30094458..30096603（-）		286		71.17		5.14
LcEXLA19	Maker00013924	Scaffold3：28963173..28964068（-）		235		57.10		5.21
LcEXLA20	Maker00013850	Scaffold3：29069173..29075111（-）		414		104.23		5.05
LcEXLA21	Maker00013746	Scaffold3：29194590..29195803（-）		247		60.62		5.20
LcEXLA22	Maker00013718	Scaffold3：29455193..29456551（-）		263		64.71		5.17
LcEXLA23	Maker00013618	Scaffold3：29098873..29100084（-）		269		66.51		5.15
LcEXLA24	Maker00005227	Scaffold11：33830538..33832189（+）		264		65.00		5.17
LcEXLA25	Maker00005193	Scaffold11：33797689..33799277（-）		298		74.46		5.13
LcEXLA26	Maker00005141	Scaffold11：33820779..33822086（-）		264		64.93		5.17
LcEXLA27	Maker00003985	Scaffold4：1360141..1361912（+）		198		49.36		5.22
LcEXLB1	Maker00038210	Scaffold12：39908175..39910085（-）		253		62.53		5.14
LcEXLB2	Maker00038088	Scaffold12：40370339..40373048（+）		256		62.82		5.15
基因名称 Gene name	基因ID Gene ID	蛋白不稳定指数 Protein instability index	脂溶指数 Lipid solubility index		疏水性 Total average hydrophobicity		亚细胞定位 Subcellular localization
LcEXPA1	Maker00039481	50.82	22.18		0.72		细胞外Extracellular
LcEXPA2	Maker00039350	51.01	22.26		0.79		细胞外Extracellular
LcEXPA3	Maker00039137	57.17	21.32		0.84		细胞外Extracellular
LcEXPA4	Maker00036366	41.14	21.35		0.62		内质网Endoplasmic
LcEXPA5	Maker00033676	48.80	24.42		0.80		细胞外Extracellular
LcEXPA6	Maker00030282	45.04	21.71		0.66		细胞外Extracellular
LcEXPA7	Maker00028836	61.26	19.97		0.92		叶绿体Chloroplast
LcEXPA8	Maker00026031	56.01	23.06		0.74		内质网Endoplasmic
LcEXPA9	Maker00018301	52.41	23.74		0.76		叶绿体Chloroplast
LcEXPA10	Maker00016100	53.80	24.25		0.77		叶绿体Chloroplast
LcEXPA11	Maker00013380	41.43	23.76		0.82		叶绿体Chloroplast
LcEXPA12	Maker00012399	56.19	18.85		0.85		细胞外Extracellular
LcEXPA13	Maker00011031	48.98	21.83		0.83		液泡Vacuole
LcEXPA14	Maker00009740	52.76	21.34		0.86		细胞外Extracellular
LcEXPA15	Maker00008644	48.99	21.79		0.71		叶绿体Chloroplast
LcEXPA16	Maker00006224	43.52	27.25		0.65		内质网Endoplasmic
LcEXPA17	Maker00003094	48.54	24.62		0.80		细胞质Cytoplasm
LcEXPA18	Maker00000768	50.04	21.24		0.81		细胞外Extracellular
LcEXPB1	Maker00000264	48.95	23.23		0.76		叶绿体Chloroplast
LcEXPB2	Maker00031725	50.10	20.07		0.74		细胞外Extracellular
LcEXPB3	Maker00008065	43.53	22.34		0.68		液泡Vacuole
LcEXLA1	Maker00037537	42.07	30.76		0.70		叶绿体Chloroplast
LcEXLA2	Maker00037507	42.46	27.01		0.61		叶绿体Chloroplast
LcEXLA3	Maker00022629	41.60	32.20		0.69		叶绿体Chloroplast
LcEXLA4	Maker00022507	40.72	32.45		0.72		细胞外Extracellular
LcEXLA5	Maker00022429	38.76	32.43		0.68		叶绿体Chloroplast
LcEXLA6	Maker00022378	36.96	32.20		0.72		叶绿体Chloroplast
LcEXLA7	Maker00020500	51.72	24.40		0.74		叶绿体Chloroplast
LcEXLA8	Maker00015327	41.21	30.27		0.71		叶绿体Chloroplast
LcEXLA9	Maker00015140	37.36	26.39		0.71		细胞外Extracellular
LcEXLA10	Maker00015111	38.05	29.94		0.64		细胞外Extracellular
LcEXLA11	Maker00015029	47.43	27.15		0.71		细胞质Cytoplasm
LcEXLA12	Maker00015000	43.60	30.08		0.66		细胞外Extracellular
LcEXLA13	Maker00014988	38.12	27.63		0.60		细胞外Extracellular
LcEXLA14	Maker00014757	39.28	29.66		0.61		叶绿体Chloroplast
LcEXLA15	Maker00014734	37.80	30.06		0.63		叶绿体Chloroplast
LcEXLA16	Maker00014637	39.87	25.97		0.66		细胞外Extracellular
LcEXLA17	Maker00014364	42.27	31.70		0.72		细胞外Extracellular
LcEXLA18	Maker00014148	39.96	29.49		0.70		细胞外Extracellular
LcEXLA19	Maker00013924	33.66	30.64		0.62		叶绿体Chloroplast
LcEXLA20	Maker00013850	41.44	26.17		0.67		内质网Endoplasmic
LcEXLA21	Maker00013746	37.90	30.90		0.62		叶绿体Chloroplast
LcEXLA22	Maker00013718	42.64	30.42		0.68		细胞外Extracellular
LcEXLA23	Maker00013618	42.52	30.61		0.70		叶绿体Chloroplast
LcEXLA24	Maker00005227	39.20	30.18		0.67		细胞外Extracellular
LcEXLA25	Maker00005193	47.09	31.10		0.72		叶绿体Chloroplast
LcEXLA26	Maker00005141	34.91	30.43		0.67		细胞外Extracellular
LcEXLA27	Maker00003985	43.48	29.12		0.64		液泡Vacuole
LcEXLB1	Maker00038210	41.61	26.48		0.68		细胞外Extracellular
LcEXLB2	Maker00038088	37.14	26.69		0.66		细胞外Extracellular

物种Species	EXPA亚家族 EXPA subfamily	EXPB亚家族 EXPB subfamily	EXLA亚家族 EXLA subfamily	EXLB亚家族 EXLB subfamily
普通丝瓜Sponge gourd	18	3	27	2
拟南芥Arabidopsis	25	6	3	1
葡萄Grape	20	4	1	4
陆地棉Upland cotton	46	8	6	12
苹果Apple	34	1	2	4
甜瓜Melon	21	3	8	3
黄瓜Cucumber	21	3	9	2