关键词: 番茄, 灰葡萄孢, MAPK级联, SlMAPKKK43

Abstract: The mitogen-activated protein kinase（MAPK）cascade plays an important role in multiple developmental and physiological processes in plants and in response to various biotic and abiotic stresses. In this study，SlMAPKKK43 overexpressed plants and CRISPR-Cas9-mediated SlMAPKKK43 knockout mutant using Agrobacterium infection genetic transformation were obtained. Through the inoculation of Botrytis cinerea with transgenic lines，we found that SlMAPKKK43 positively regulated the tolerance of tomato to Botrytis cinerea. In order to further elucidate the molecular mechanism of SlMAPKKK43 regulating the resistance of gray mold，the known gray mold regulators SlMKK2 and SlMKK4 were screened by pull down-MS technology，and in vitro phosphorylation assay was used to preliminarily verify that SlMAPKKK43 could phosphorylate SlMKK2 and SlMKK4.

Key words: tomato, Botrytis cinerea, MAPK cascade, SlMAPKKK43