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园艺学报 ›› 2024, Vol. 51 ›› Issue (2): 229-238.doi: 10.16420/j.issn.0513-353x.2023-0694

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

番茄抗旱性的全基因组关联分析

董舒超1,2,洪 骏3,凌嘉怡1,4,谢紫欣1,5,张胜军6,赵丽萍1,2,宋刘霞1,2,王银磊1,2,赵统敏1,2,*   

  1. 1江苏省农业科学院蔬菜研究所,南京 210014;2江苏省高效园艺作物遗传改良重点实验室,南京 210014;3沙漠农业研究中心,阿卜杜拉国王科技大学,沙特吉达 23955;4扬州大学园艺园林学院,江苏扬州 225100;5南京农业大学园艺学院,南京 210095;6伊犁哈萨克自治州农业科学研究所,新疆伊宁 835000
  • 出版日期:2024-02-25 发布日期:2024-02-26
  • 基金资助:
    江苏省自然科学基金青年基金项目(BK20220743);国家自然科学基金青年科学基金项目(32202489);江苏省重点研发计划现代农业项目(BE2022339);江苏省种业振兴揭榜挂帅项目(JBGS(2021)066);南京市品种选育专项(202210023)

Genome-wide Association Studies of Drought Tolerance in Tomato

DONG Shuchao1,2,HONG Jun3,LING Jiayi1,4,XIE Zixin1,5,ZHANG Shengjun6,ZHAO Liping1,2,SONG Liuxia1,2,WANG Yinlei1,2,and ZHAO Tongmin1,2,*   

  1. 1Institute of Vegetable Crop,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China;3Center for Desert Agriculture,King Abdullah University of Science and Technology,Jeddah 23955,Saudi Arabia;4College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225100,China;5College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;6Institute of Agricultural Sciences of Ili Kazakh Autonomous Prefecture,Yining,Xinjiang 835000,China
  • Published:2024-02-25 Online:2024-02-26

摘要: 测定了301份番茄(Solanum lycopersicum L.)种质的叶片在离体24 h后的相对失水率,以鉴定其抗旱能力,并进行了全基因组关联分析(genome-wide association study,GWAS)。共检测到3个与番茄抗旱性显著关联的SNP位点,分别为S02.1158758、S02.1160893和S05.1426646,单个位点可以解释4.08% ~ 18.97%的表型变异。挖掘到3个与番茄抗旱性显著关联的主效SNP位点和7个候选基因,有助于解析番茄抗旱性的遗传基础及其调控机制,为番茄抗逆遗传改良奠定了基础。

关键词: 番茄, 抗旱性, 全基因组关联分析, 候选基因

Abstract: To conduct genome-wide association studies(GWAS)of drought tolerance,the relative water loss of detached leaves was analyzed after 24 h dehydration in 301 tomato(Solanum lycopersicum L.)accessions. A total number of three SNP sites(S02.1158758,S02.1160893,and S05.1426646)significantly associated with the drought tolerance of tomato accessions were identified by GWAS. Each single locus could explain 4.08%–18.97% of the phenotypic variation. Taken together,three SNP sites and seven candidate genes significantly associated with drought tolerance in tomato were identified in this study,which will help unravel the genetic and regulatory mechanisms of tomato drought tolerance,as well as establish a foundation for the genetic improvement of tomato stress tolerance.

Key words: tomato, drought tolerance, genome-wide association studies, candidate gene

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