中国菊花遗传育种60年回顾与展望

doi:10.16420/j.issn.0513-353x.2022-0859

摘要/Abstract

摘要：

简要介绍了菊花在中国的发展历程,回顾了60年来中国在菊花种质资源、重要性状遗传和形成的分子机制、传统育种和现代生物技术育种进展及取得的主要成就,阐述了转基因、基因编辑、分子标记辅助选择、多组学等现代生物学技术在中国菊花遗传育种研究中的应用现状,并针对存在的主要问题提出育种发展建议。

关键词: 菊花, 种质资源, 遗传育种, 重要性状, 生物技术, 分子机制

Abstract:

This review briefly summaries the processes for chrysanthemum development,and recalls the progress and main achievements that have been made in the germplasm resources,inheritance and molecular mechanisms underlying important traits formation,traditional breeding as well as modern biotechnology breeding programs during the last six decades in China. We also summarize the application status of modern biological technologies such as transgenic,genome editing,molecular marker-assisted selection,and multi-omics in the area of chrysanthemum genetics and breeding,and propose suggestions for the development of chrysanthemum genetics and breeding accordingly.

Key words: chrysanthemum, germplasm resources, genetic breeding, important trait, biotechnology, molecular mechanism

中图分类号:

S682.11

苏江硕, 贾棣文, 王思悦, 张飞, 蒋甲福, 陈素梅, 房伟民, 陈发棣. 中国菊花遗传育种60年回顾与展望[J]. 园艺学报, 2022, 49(10): 2143-2162.

SU Jiangshuo, JIA Diwen, WANG Siyue, ZHANG Fei, JIANG Jiafu, CHEN Sumei, FANG Weimin, and CHEN Fadi. Retrospection and Prospect of Chrysanthemum Genetic Breeding for Last Six Decades in China[J]. Acta Horticulturae Sinica, 2022, 49(10): 2143-2162.

图/表 5

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性状 Trait	基因 Gene	供体 Used for transgene isolated	受体 For genetic transformation	获得性状 Changed trait	文献 Reference
花色 Flower color	CmMYB#7	'神马''Jinba'	'神马''Jinba'	负调控花色苷合成Negative regulator of anthocyanin biosynthesis	Xiang et al.,2019
	CmMYB012	'南农粉翠' 'Nannong Fencui'	'南农粉翠' 'Nannong Fencui'	花色变浅 Pink flower color becomes lighter	Zhou et al.,2021
	CmMYB21	'粉安娜' 'Anastasia Pink'	烟草 Tobacco	花色变浅Pink flower color becomes lighter	Wang et al.,2022b
	CmMYB9a	'粉安娜' 'Anastasia Pink'	烟草 Tobacco	花色加深 Pink flower color becomes darker	Wang et al.,2022c
	CmbHLH16	'南农粉翠' 'Nannong Fencui'	'南农粉翠' 'Nannong Fencui'	在不同红光和远红光比例下调控花色 Regulates flower color under different red and far-red lights	Zhou et al.,2022
花型 Flower shape	CmXTH1-4	'杭白菊''Hangbaiju'	'杭白菊''Hangbaiju'	促进舌状花花瓣伸长 Promote elongation of ray florets	Wen et al.,2017
	CmTCP20	'神马''Jinba'	'神马''Jinba'	促进舌状花花瓣伸长 Promote elongation of ray florets	Wang et al.,2019
	CmYAB1	'神马''Jinba'	'神马''Jinba'	改变舌状花翻卷程度和花型 Changes in petal curvature and inflorescence morphology	Ding et al.,2019
	CmCYC2a-2f	'毛香玉''Maoxiangyu'	甘菊 Chrysanthemun lavandulifolium	调控舌状花形成和花对称性 Regulation of ray florets formation and flower symmetry	Yuan et al.,2020
	CmBES1;CmCUC2/ 3	'神马''Jinba'	'神马''Jinba'	影响舌状花融合程度Affection in merged degree of the ray florets	Cheng et al.,2020; 夏伟康,2020
株型 Plant	CmERF053	'神马''Jinba'	拟南芥 Arabidopsis	促进分枝和侧根形成 Promote branching and lateral root formation	Nie et al.,2018
architecture	CmMAX1	'神马''Jinba'	拟南芥 Arabidopsis	减少分枝数Reduce branch numbers	Dong et al.,2018
	CmSWEET17	'神马''Jinba'	'神马''Jinba'	促进腋芽生长Promote axillary bud outgrowth	刘伟鑫,2019
	CmHLB,CmKNAT7	'神马''Jinba'	'神马''Jinba'	植株矮化,茎秆增粗 Dwarf plants,thickened stems	Zhao et al.,2022
	CmDIV	'毛香玉''Maoxiangyu'	拟南芥 Arabidopsis	植株较低矮,叶片小且圆 Plants become shorter,with smaller and rounder leaves	刘轶奇,2016

性状 Trait	基因 Gene	供体 Used for transgene isolated	受体 For genetic transformation	获得性状 Changed trait	文献 Reference
花色 Flower color	CmMYB#7	'神马''Jinba'	'神马''Jinba'	负调控花色苷合成Negative regulator of anthocyanin biosynthesis	Xiang et al.,2019
	CmMYB012	'南农粉翠' 'Nannong Fencui'	'南农粉翠' 'Nannong Fencui'	花色变浅 Pink flower color becomes lighter	Zhou et al.,2021
	CmMYB21	'粉安娜' 'Anastasia Pink'	烟草 Tobacco	花色变浅Pink flower color becomes lighter	Wang et al.,2022b
	CmMYB9a	'粉安娜' 'Anastasia Pink'	烟草 Tobacco	花色加深 Pink flower color becomes darker	Wang et al.,2022c
	CmbHLH16	'南农粉翠' 'Nannong Fencui'	'南农粉翠' 'Nannong Fencui'	在不同红光和远红光比例下调控花色 Regulates flower color under different red and far-red lights	Zhou et al.,2022
花型 Flower shape	CmXTH1-4	'杭白菊''Hangbaiju'	'杭白菊''Hangbaiju'	促进舌状花花瓣伸长 Promote elongation of ray florets	Wen et al.,2017
	CmTCP20	'神马''Jinba'	'神马''Jinba'	促进舌状花花瓣伸长 Promote elongation of ray florets	Wang et al.,2019
	CmYAB1	'神马''Jinba'	'神马''Jinba'	改变舌状花翻卷程度和花型 Changes in petal curvature and inflorescence morphology	Ding et al.,2019
	CmCYC2a-2f	'毛香玉''Maoxiangyu'	甘菊 Chrysanthemun lavandulifolium	调控舌状花形成和花对称性 Regulation of ray florets formation and flower symmetry	Yuan et al.,2020
	CmBES1;CmCUC2/ 3	'神马''Jinba'	'神马''Jinba'	影响舌状花融合程度Affection in merged degree of the ray florets	Cheng et al.,2020; 夏伟康,2020
株型 Plant	CmERF053	'神马''Jinba'	拟南芥 Arabidopsis	促进分枝和侧根形成 Promote branching and lateral root formation	Nie et al.,2018
architecture	CmMAX1	'神马''Jinba'	拟南芥 Arabidopsis	减少分枝数Reduce branch numbers	Dong et al.,2018
	CmSWEET17	'神马''Jinba'	'神马''Jinba'	促进腋芽生长Promote axillary bud outgrowth	刘伟鑫,2019
	CmHLB,CmKNAT7	'神马''Jinba'	'神马''Jinba'	植株矮化,茎秆增粗 Dwarf plants,thickened stems	Zhao et al.,2022
	CmDIV	'毛香玉''Maoxiangyu'	拟南芥 Arabidopsis	植株较低矮,叶片小且圆 Plants become shorter,with smaller and rounder leaves	刘轶奇,2016

基因 Gene	供体Used for transgene isolated		受体For genetic transformation	获得性状 Changed trait		途径 Pathway		文献 Reference
DenFUL	甘菊Chrysanthemun lavandulifolium		拟南芥 Arabidopsis	提前开花 Early-flowering		不详Unknown		王翊,2013
CmBBX24	'Fall Color'		'Fall Color'	延迟开花Late-flowering		光周期和赤霉素途径 Photoperiod and GA pathways		Yang et al.,2014
AtCO	拟南芥 Arabidopsis		'火焰''Huoyan'	提前开花Early-flowering		光周期途径 Photoperiod pathway		宋凤艳,2016
CmFTL1	'神马''Jinba'		'神马''Jinba'	提前开花Early-flowering		光周期途径 Photoperiod pathway		Mao et al.,2016
CmFTL2	'优香''Yuuka'		'优香''Yuuka'	提前开花Early-flowering		光周期途径 Photoperiod pathway		Sun et al.,2017
CmNF-YB8	'Fall Color'		'Fall Color'	延迟开花Late-flowering		年龄途径 Aging pathway		Wei et al.,2017
ClPHYB	甘菊Chrysanthemun lavandulifolium		拟南芥 Arabidopsis	推迟开花Late-flowering		光周期途径 Photoperiod pathway		马朝峰,2019
CmBBX13	'优香''Yuuka'		拟南芥 Arabidopsis	推迟开花Late-flowering		独立于光周期途径 Independently of the photoperiod pathway		Ping et al.,2019
CmTPL1-2	'神马''Jinba'		'神马''Jinba'	推迟开花Late-flowering		不详Unknown		Zhang et al.,2019
CmBBX8	'优香''Yuuka'		'优香''Jinba'	提前开花Early-flowering		光周期途径 Photoperiod pathway		Wang et al.,2020b
CmAP1L1	'神马''Jinba'		'神马''Jinba'	提前开花Early-flowering		不详Unknown		Zhao et al.,2020
CmJAZ1-like	'神马''Jinba'	'神马''Jinba'			延迟开花Late-flowering		不详Unknown	Guan et al.,2021
CmERF110,CmFLK	'神马''Jinba'	'神马''Jinba'			提前开花Early-flowering		自主途径Autonomous pathway	Huang et al.,2022a

基因 Gene	供体Used for transgene isolated		受体For genetic transformation	获得性状 Changed trait		途径 Pathway		文献 Reference
DenFUL	甘菊Chrysanthemun lavandulifolium		拟南芥 Arabidopsis	提前开花 Early-flowering		不详Unknown		王翊,2013
CmBBX24	'Fall Color'		'Fall Color'	延迟开花Late-flowering		光周期和赤霉素途径 Photoperiod and GA pathways		Yang et al.,2014
AtCO	拟南芥 Arabidopsis		'火焰''Huoyan'	提前开花Early-flowering		光周期途径 Photoperiod pathway		宋凤艳,2016
CmFTL1	'神马''Jinba'		'神马''Jinba'	提前开花Early-flowering		光周期途径 Photoperiod pathway		Mao et al.,2016
CmFTL2	'优香''Yuuka'		'优香''Yuuka'	提前开花Early-flowering		光周期途径 Photoperiod pathway		Sun et al.,2017
CmNF-YB8	'Fall Color'		'Fall Color'	延迟开花Late-flowering		年龄途径 Aging pathway		Wei et al.,2017
ClPHYB	甘菊Chrysanthemun lavandulifolium		拟南芥 Arabidopsis	推迟开花Late-flowering		光周期途径 Photoperiod pathway		马朝峰,2019
CmBBX13	'优香''Yuuka'		拟南芥 Arabidopsis	推迟开花Late-flowering		独立于光周期途径 Independently of the photoperiod pathway		Ping et al.,2019
CmTPL1-2	'神马''Jinba'		'神马''Jinba'	推迟开花Late-flowering		不详Unknown		Zhang et al.,2019
CmBBX8	'优香''Yuuka'		'优香''Jinba'	提前开花Early-flowering		光周期途径 Photoperiod pathway		Wang et al.,2020b
CmAP1L1	'神马''Jinba'		'神马''Jinba'	提前开花Early-flowering		不详Unknown		Zhao et al.,2020
CmJAZ1-like	'神马''Jinba'	'神马''Jinba'			延迟开花Late-flowering		不详Unknown	Guan et al.,2021
CmERF110,CmFLK	'神马''Jinba'	'神马''Jinba'			提前开花Early-flowering		自主途径Autonomous pathway	Huang et al.,2022a

类型 Type	基因 Gene		供体Used for transgene isolated	受体For genetic transformation		获得性状 Changed trait	文献 Reference
非生物胁迫	DgNAC1	'神马''Jinba'			'神马''Jinba'	耐旱性Drought tolerance	Zhao et al.,2018
Abiotic stress	CmBBX19,CmABF3	'Fall Color'			'Fall Color'	耐旱性Drought tolerance	Xu et al.,2020
	CgDREB22	'纽9717''Niu9717'			烟草 Tobacco	耐旱性Drought tolerance	杨伊如,2020
	CmRH56	'Fall Color'			菊花C. morifolium	耐旱性Drought tolerance	Zhang et al.,2022
	CmSOS1,CmRCD1	'神马''Jinba'			'神马''Jinba'	耐涝性Waterlogging tolerance	Wang et al.,2020b
	DlNAC1		甘菊 Chrysanthemun lavandulifolium	烟草 Tobacco		耐热性Heat tolerance	朱凯等,2017
	eIF5B1		拟南芥 Arabidopsis	菊花 C. morifolium		耐热性Heat tolerance	Wei et al.,2019
	DgMYB1		'神马''Jinba'	'神马''Jinba'		耐寒性Cold tolerance	何玲,2019
	CmICE2		'神马''Jinba'	拟南芥 Arabidopsis		耐寒性Cold tolerance	Zhang et al.,2020
	DgC3H1		菊花 C. morifolium	菊花 C. morifolium		耐寒性Cold tolerance	Bai et al.,2021
	CmHSFA4;DgWRKY2; DgMBF1,DgJAZ1		'神马''Jinba'	'神马''Jinba'		耐盐性Salt tolerance	Li et al.,2018;He et al.,2018;Zhao et al.,2019
	ClSND1.1,ClVND1,ClBRN1		甘菊 Chrysanthemun lavandulifolium	拟南芥 Arabidopsis		耐盐性Salt tolerance	王海等,2021
	CmBBX24		'Fall Color'	'Fall Color'		耐干旱和低温 Drought and cold tolerance	Yang et al.,2014
	CdICE1		异色菊 C. dichrum	'神马''Jinba'		干旱、低温、盐 Drought,cold and salt tolerance	Chen et al.,2012
	DmDREBa/b		菊花C. morifolium	烟草 Tobacco		干旱、低温、盐 Drought,cold and salt tolerance	武剑等,2016
	CmDREBa-2		'C029'	'C029'		耐旱性和抗白锈病 Drought and white rust resistance	毕蒙蒙等,2019
	CgbZIP		'纽9717' 'Niu9717'	烟草 Tobacco		耐盐性和耐旱性 Salt and drought tolerance	Liu et al.,2022
生物胁迫 Biotic stress	CmWRKY15;CmWRKY33.1; CmMLO17,CmKIC		'神马''Jinba'	'神马''Jinba'		黑斑病抗性 Black spot disease resistance	Fan et al.,2015;Liu et al.,2020;辛静静,2020
	CmMYB19;CmTPS2/3;CmMYB15		'神马''Jinba'	'神马''Jinba'		抗蚜虫性Aphid resistance	Wang et al.,2017;王威姣,2019;An et al.,2019