景天酸代谢植物分子生物学研究进展及应用潜力

doi:10.16420/j.issn.0513-353x.2021-0835

园艺学报 ›› 2022, Vol. 49 ›› Issue (12): 2597-2610.doi: 10.16420/j.issn.0513-353x.2021-0835

景天酸代谢植物分子生物学研究进展及应用潜力

李俊璋¹, 秦源²^,³^,⁴, 肖强¹, 安昌²^,⁴, 廖静怡³, 郑平²^,^*()

¹福建农林大学农学院，福州 350000
²福建农林大学海峡联合研究院，福州 350000
³福建农林大学生命科学学院，福州 350000
⁴广西大学农学院，南宁 530000

收稿日期:2022-04-22 修回日期:2022-08-31 出版日期:2022-12-25 发布日期:2023-01-02
通讯作者: 郑平 E-mail:zhengping@fafu.edu.cn
基金资助:
国家自然科学基金项目(31970333);福建省科技厅产学研项目(2019N5008);福建省自然科学基金面上基金（高校联合资助）项目(2020J01594);广西壮族自治区特聘专家项目([2018]39)

Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering

LI Junzhang¹, QIN Yuan²^,³^,⁴, XIAO Qiang¹, AN Chang²^,⁴, LIAO Jingyi³, ZHENG Ping²^,^*()

¹College of Agriculture，Fujian Agriculture and Forestry University，Fuzhou 350000，China
²Haixia Institute of Science and Technology，Fujian Agriculture and Forestry University，Fuzhou 350000，China
³College of Life Science，Fujian Agriculture and Forestry University，Fuzhou 350000，China
⁴College of Agriculture，Guangxi University，Nanning 530000，China

Received:2022-04-22 Revised:2022-08-31 Online:2022-12-25 Published:2023-01-02
Contact: ZHENG Ping E-mail:zhengping@fafu.edu.cn

摘要/Abstract

摘要：

景天酸代谢（crassulacean acid metabolism，CAM）植物在夜间固定CO₂且具有较高的水分利用效率的碳集中机制（carbon concentrating mechanism）。将CAM光合作用途径“搬进”C₃作物中提高C₃作物水分利用效率以增强其抗旱性（即CAM工程化），在未来农业中具有重要的应用潜力。系统认识CAM植物光合作用途径及其相关调控机理，是开发和利用CAM光合作用途径的重要理论基础。目前，组学和分子生物学的发展大大促进了CAM植物相关研究。CAM途径关键基因及其功能逐渐被揭示，10多种CAM植物基因组被破译，转录因子、激素、miRNA、lncRNA、可变剪切、DNA甲基化等多种因素参与CAM途径调控过程。本文中主要对CAM植物分子生物学和组学研究进展进行综述，包括CAM植物生理特性研究、CAM植物组学资源、CAM途径相关基因及其调控等，并对未来的研究进行了展望。

关键词: 光合作用途径, 景天酸代谢, 多组学, 分子机制, 转录调控, 水分利用率

Abstract:

Crassulacean acid metabolism photosynthetic pathway is a kind of carbon concentrating mechanism that evolved from C3 photosynthesis and it is characterized by nocturnal CO₂ fixation and high water use efficiency. Currently，there are growing interests in improving WUE to enhance drought resistance by“transferring”CAM pathway into C₃ crops，also called‘CAM engineering’. CAM engineering has significant application potential in agriculture，while systematical investigations of CAM photosynthetic pathway / plants are a prerequisite to its progression. The wide applications of multi-omics and molecular biology methods have extensively promoted CAM plants related studies. CAM pathway-related key genes and their functions are being gradually revealed，and many CAM plant genomes have been published. Diverse mechanisms are involved in the regulation of the CAM pathway, including transcription factors，hormones，miRNA，lncRNA，alternative splicing，and DNA methylation This article mainly focuses on the latest progress of molecular biology and omics studies on CAM plants. In addtion，future research prospective and study interests are also discussed to guide further investigations on CAM plants.

Key words: crassulacean acid, metabolism, multi-omics, molecular mechanism, transcriptional regulation, water use efficiency

中图分类号:

S6
Q 945.11

李俊璋, 秦源, 肖强, 安昌, 廖静怡, 郑平. 景天酸代谢植物分子生物学研究进展及应用潜力[J]. 园艺学报, 2022, 49(12): 2597-2610.

LI Junzhang, QIN Yuan, XIAO Qiang, AN Chang, LIAO Jingyi, ZHENG Ping. Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering[J]. Acta Horticulturae Sinica, 2022, 49(12): 2597-2610.

图/表 2

参考文献 68

[1]	Abraham P E, Hurtado Castano N, Cowan-Turner D, Barnes J, Poudel S, Hettich R, Flütsch S, Santelia D, Borland A M. 2020. Peeling back the layers of crassulacean acid metabolism:functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes. The Plant Journal, 103 (2):869-888. doi: 10.1111/tpj.14757 pmid: 32314451
[2]	Amin A B, Rathnayake K N, Yim W C, Garcia T M, Wone B, Cushman J C, Wone B W. 2019. Crassulacean acid metabolism abiotic stress-responsive transcription factors:a potential genetic engineering approach for improving crop tolerance to abiotic stress. Frontiers in Plant Science, 10:129. doi: 10.3389/fpls.2019.00129 URL
[3]	Bai Y, Dai X, Li Y, Wang L, Li W, Liu Y, Cheng Y, Qin Y. 2019. Identification and characterization of pineapple leaf lncRNAs in crassulacean acid metabolism(CAM)photosynthesis pathway. Scientific Reports, 9 (1):6658. doi: 10.1038/s41598-019-43088-8 URL
[4]	Bedre R, Irigoyen S, Petrillo E, Mandadi K K. 2019. New era in plant alternative splicing analysis enabled by advances in high-throughput sequencing(HTS)technologies. Frontiers in Plant Science, 10:740. doi: 10.3389/fpls.2019.00740 URL
[5]	Borland A M, Griffiths H, Hartwell J, Smith J A. C. 2009. Exploiting the potential of plants with crassulacean acid metabolism for bioenergy production on marginal lands. Journal of Experimental Botany, 60 (10):2879-2896. doi: 10.1093/jxb/erp118 pmid: 19395392
[6]	Borland A M, Hartwell J, Weston D J, Schlauch K A, Tschaplinski T J, Tuskan G A, Yang X, Cushman J C. 2014. Engineering crassulacean acid metabolism to improve water-use efficiency. Trends in Plant Science, 19 (5):327-338. doi: 10.1016/j.tplants.2014.01.006 pmid: 24559590
[7]	Boxall S F, Dever L V, Kneřová J, Gould P D, Hartwell J. 2017. Phosphorylation of phosphoenolpyruvate carboxylase is essential for maximal and sustained dark CO₂ fixation and core circadian clock operation in the obligate crassulacean acid metabolism species Kalanchoë fedtschenko. The Plant Cell, 29 (10):2519-2536. doi: 10.1105/tpc.17.00301 URL
[8]	Boxall S F, Kadu N, Dever L V, Kneřová J, Waller J L, Gould P J D, Hartwell J. 2020. Kalanchoë PPC 1 is essential for crassulacean acid metabolism and the regulation of core circadian clock and guard cell signaling genes. The Plant Cell, 32 (4):1136-1160. doi: 10.1105/tpc.19.00481 URL
[9]	Brilhaus D, Bräutigam A, Mettler-Altmann T, Winter K, Weber A P M. 2016. Reversible burst of transcriptional changes during induction of crassulacean acid metabolism in Talinum triangulare. Plant Physiology, 170 (1):102-122. doi: 10.1104/pp.15.01076 pmid: 26530316
[10]	Bronson D R, English N B, Dettman D L, Williams D G. 2011. Seasonal photosynthetic gas exchange and water-use efficiency in a constitutive CAM plant,the giant saguaro cactus(Carnegiea gigantea). Oecologia, 167:861. doi: 10.1007/s00442-011-2021-1 pmid: 21822726
[11]	Brulfert J, Kluge M, Güçlü S, Queiroz O. 1988. Interaction of photoperiod and drought as CAM inducing factors in Kalanchoë blossfeldiana Poelln,cv. Tom Thumb. Journal of Plant Physiology, 133 (2):222-227. doi: 10.1016/S0176-1617(88)80141-X URL
[12]	Cai J, Liu X, Vanneste K, Proost S, Tsai W C, Liu K W, Chen L J, He Y, Xu Q, Bian C, Zheng Z, Sun F, Liu W, Hsiao Y Y, Pan Z J, Hsu C C, Yang Y P, Hsu Y C, Chuang Y C, Dievart A, Dufayard J F, Xu X, Wang J Y, Wang J, Xiao X J, Zhao X M, Du R, Zhang G Q, Wang M, Su Y Y, Xie G C, Liu G H, Li L Q, Huang L Q, Luo Y B, Chen H H, van de Peer Y, Liu Z J. 2015. The genome sequence of the orchid Phalaenopsis equestris. Nature Genetics, 47 (1):65-72. doi: 10.1038/ng.3149 URL
[13]	Ceusters J, Borland A M, Taybi T, Frans M, Godts C, de Proft M. P. 2014. Light quality modulates metabolic synchronization over the diel phases of crassulacean acid metabolism. Journal of Experimental Botany, 65 (13):3705-3714. doi: 10.1093/jxb/eru185 pmid: 24803500
[14]	Ceusters N, Borland A M, Ceusters J. 2021. How to resolve the enigma of diurnal malate XXX emobilization from the vacuole in plants with crassulacean acid metabolism? New Phytologist, 229 (6):3116-3124. doi: 10.1111/nph.17070 URL
[15]	Ceusters N, Frans M, van den Ende W, Ceusters J. 2019a. Maltose Processing and Not β-amylase activity curtails hydrolytic starch degradation in the CAM orchid Phalaenopsis. Frontiers in Plant Science, 10:1386. doi: 10.3389/fpls.2019.01386 URL
[16]	Ceusters N, Luca S, Feil R, Claes J E, Lunn J E, van den Ende W, Ceusters J. 2019b. Hierarchical clustering reveals unique features in the diel dynamics of metabolites in the CAM orchid Phalaenopsis. Journal of Experimental Botany, 70 (12):3269-3281. doi: 10.1093/jxb/erz170 URL
[17]	Chao Y T, Chen W C, Chen C Y, Ho H Y, Yeh C H, Kuo Y T, Su C L, Yen S H, Hsueh H Y, Yeh J H, Hsu H L, Tsai Y H, Kuo T Y, Chang S B, Chen K Y, Shih M C. 2018. Chromosome-level assembly,genetic and physical mapping of Phalaenopsis aphrodite genome provides new insights into species adaptation and resources for orchid breeding. Plant Biotechnology Journal, 16 (12):2027-2041. doi: 10.1111/pbi.12936 URL
[18]	Chen L Y, VanBuren R, Paris M, Zhou H, Zhang X, Wai C M, Yan H, Chen S, Alonge M, Ramakrishnan S, Liao Z, Liu J, Lin J, Yue J., Fatima M, Lin Z, Zhang J, Huang L, Wang H, Hwa T Y, Kao S M, Choi J Y, Sharma A, Song J, Wang L, Yim W C, Cushman J C, Paull R E, Matsumoto T, Qin Y, Wu Q, Wang J, Yu Q, Wu J, Zhang S, Boches P, Tung C W, Wang M L, Coppens d’Eeckenbrugge G, Sanewski G M, Purugganan M D, Schatz M C, Bennetzen J L, Lexer C, Ming R. 2019. The bracteatus pineapple genome and domestication of clonally propagated crops. Nature Genetics, 51 (10):1549-1558. doi: 10.1038/s41588-019-0506-8 pmid: 31570895
[19]	Chen L Y, Xin Y, Wai C M, Liu J, Ming R. 2020. The role of cis-elements in the evolution of crassulacean acid metabolism photosynthesis. Horticulture Research, 7 (1):5. doi: 10.1038/s41438-019-0229-0 URL
[20]	Chomthong M, Griffiths H. 2020. Model approaches to advance crassulacean acid metabolism system integration. The Plant Journal, 101 (4):951-963. doi: 10.1111/tpj.14691 pmid: 31943394
[21]	Cushman J C. 2001. Crassulacean acid metabolism. A plastic photosynthetic adaptation to arid environments. Plant physiology, 127 (4):1439-1448. pmid: 11743087
[22]	Dever L V, Boxall S F, Kneřová J, Hartwell J. 2015. Transgenic perturbation of the decarboxylation phase of crassulacean acid metabolism alters physiology and metabolism but has only a small effect on growth. Plant Physiology, 167 (1):44-59. doi: 10.1104/pp.114.251827 pmid: 25378692
[23]	Edwards E J. 2019. Evolutionary trajectories,accessibility and other metaphors:the case of C(4)and CAM photosynthesis. New Phytologist, 223 (4):1742-1755. doi: 10.1111/nph.15851 pmid: 30993711
[24]	Ermakova M, Danila F R, Furbank R T, von Caemmerer S. 2020. On the road to C 4 rice:advances and perspectives. The Plant Journal, 101 (4):940-950. doi: 10.1111/tpj.14562 pmid: 31596523
[25]	Ferrari R C, Bittencourt P P, Rodrigues M A, Moreno-Villena J J, Alves F R, Gastaldi V D, Boxall S F, Dever L V, Demarco D, Andrade S C. 2020. C4 and crassulacean acid metabolism within a single leaf:deciphering key components behind a rare photosynthetic adaptation. New Phytologist, 225 (4):1699-1714. doi: 10.1111/nph.16265 pmid: 31610019
[26]	Grams T E E, Thiel S. 2002. High light‐induced switch from C3‐photosynthesis to Crassulacean acid metabolism is mediated by UV‐A/blue light. Journal of Experimental Botany, 53 (373):1475-1483.
[27]	Guan Q, Kong W, Zhu D, Zhu W, Dufresne C, Tian J, Chen S. 2021. Comparative proteomics of Mesembryanthemum crystallinum guard cells and mesophyll cells in transition from C3 to CAM. Journal of Proteomics, 231:104019. doi: 10.1016/j.jprot.2020.104019 URL
[28]	Ha J, Shim S, Lee T, Kang Y J, Hwang W J, Jeong H, Laosatit K, Lee J, Kim S K, Satyawan D, Lestari P, Yoon M Y, Kim M Y, Chitikineni A, Tanya P, Somta P, Srinives P, Varshney R K, Lee S H. 2019. Genome sequence of Jatropha curcas L.,a non-edible biodiesel plant,provides a resource to improve seed-related traits. Plant Biotechnology Journal, 17 (2):517-530. doi: 10.1111/pbi.12995 URL
[29]	Hashiguchi A, Yamaguchi H, Hitachi K, Watanabe K. 2021. An optimized protein extraction method for gel-free proteomic analysis of opuntia ficus-Indica. Plants, 10 (1):115. doi: 10.3390/plants10010115 URL
[30]	Heyduk K, Hwang M, Albert V, Silvera K, Lan T, Farr K, Chang T H, Chan M T, Winter K, Leebens-Mack J. 2019a. Altered gene regulatory networks are associated with the transition from C 3 to crassulacean acid metabolism in Erycina(Oncidiinae:Orchidaceae). Frontiers in Plant Science, 9:2000. doi: 10.3389/fpls.2018.02000 URL
[31]	Heyduk K, Moreno-Villena J J, Gilman I S, Christin P A, Edwards E J. 2019b. The genetics of convergent evolution: insights from plant photosynthesis. Nature Reviews Genetics, 20 (8):485-493. doi: 10.1038/s41576-019-0107-5 URL
[32]	Heyduk K, Ray J N, Ayyampalayam S, Leebens-Mack J. 2018. Shifts in gene expression profiles are associated with weak and strong crassulacean acid metabolism. American Journal of Botany, 105:587-601. doi: 10.1002/ajb2.1017 pmid: 29746718
[33]	Heyduk K, Ray J N, Leebens-Mack J. 2021. Leaf anatomy is not correlated to CAM function in a C3 + CAM hybrid species,Yucca gloriosa. Annals of Botany, 127 (4):437-449. doi: 10.1093/aob/mcaa036 URL
[34]	Kong W, Yoo M J, Zhu D, Noble J D, Kelley T M, Li J, Kirst M, Assmann S M, Chen S. 2020. Molecular changes in Mesembryanthemum crystallinum guard cells underlying the C3 to CAM transition. Plant Molecular Biology, 103:653-667. doi: 10.1007/s11103-020-01016-9 URL
[35]	Lim S D, Mayer J A, Yim W C, Cushman J C. 2020. Plant tissue succulence engineering improves water-use efficiency,water-deficit stress attenuation and salinity tolerance in Arabidopsis. The Plant Journal, 103:1049-1072. doi: 10.1111/tpj.14783 URL
[36]	Lin H, Arrivault S, Coe R A, Karki S, Covshoff S, Bagunu E, Lunn J E, Stitt M, Furbank R T, Hibberd J M, Quick W P. 2020. A partial C 4 photosynthetic biochemical pathway in rice. Frontiers in Plant Science, 11:564463. doi: 10.3389/fpls.2020.564463 URL
[37]	Liu D, Chen M, Mendoza B, Cheng H, Hu R, Li L, Trinh C T, Tuskan G A, Yang X. 2019. CRISPR/Cas9-mediated targeted mutagenesis for functional genomics research of crassulacean acid metabolism plants. Journal of Experimental Botany, 70 (22):6621-6629. doi: 10.1093/jxb/erz415 pmid: 31562521
[38]	Liu D, Palla K J, Hu R, Moseley R C, Mendoza C, Chen M, Abraham P E, Labbé J L, Kalluri U C, Tschaplinski T J, Cushman J C, Borland A M, Tuskan G A, Yang X. 2018. Perspectives on the basic and applied aspects of crassulacean acid metabolism(CAM)research. Plant Science, 274:394-401. doi: 10.1016/j.plantsci.2018.06.012 URL
[39]	Lüttge U. 2010. Ability of crassulacean acid metabolism plants to overcome interacting stresses in tropical environments. AoB PLANTS,doi:10.1093/aobpla/plq005. doi: 10.1093/aobpla/plq005 URL
[40]	Maleckova E, Brilhaus D, Wrobel T J, Weber A P M. 2019. Transcript and metabolite changes during the early phase of abscisic acid-mediated induction of crassulacean acid metabolism in Talinum triangulare. Journal of Experimental Botany, 70 (22):6581-6596. doi: 10.1093/jxb/erz189 pmid: 31111894
[41]	Ming R, van Buren R, Wai C M, Tang H, Schatz M C, Bowers J E, Lyons E, Wang M L, Chen J, Biggers E, Zhang J, Huang L, Zhang L, Miao W, Zhang J, Ye Z, Miao C, Lin Z, Wang H, Zhou H, Yim W C, Priest H D, Zheng C, Woodhouse M, Edger P P, Guyot R, Guo H-B, Guo H, Zheng G, Singh R, Sharma A, Min X, Zheng Y, Lee H, Gurtowski J, Sedlazeck F J, Harkess A, McKain M R, Liao Z, Fang J, Liu J, Zhang X, Zhang Q, Hu W, Qin Y, Wang K, Chen L Y, Shirley N, Lin Y-R, Liu L-Y, Hernandez A G, Wright C L, Bulone V, Tuskan G A, Heath K, Zee F, Moore P H, Sunkar R, Leebens-Mack J H, Mockler T, Bennetzen J L, Freeling M, Sankoff D, Paterson A H, Zhu X, Yang X, Smith J A C, Cushman J C, Paull R E, Yu Q. 2015. The pineapple genome and the evolution of CAM photosynthesis. Nature Genetics, 47 (12):1435-1442. doi: 10.1038/ng.3435 pmid: 26523774
[42]	Niu Z, Zhu F, Fan Y, Li C, Zhang B, Zhu S, Hou Z, Wang M, Yang J, Xue Q, Liu W, Ding X. 2021. The chromosome-level reference genome assembly for Dendrobium officinale and its utility of functional genomics research and molecular breeding study. Acta Pharmaceutica Sinica B, 11 (7):2080-2092. doi: 10.1016/j.apsb.2021.01.019 URL
[43]	Ping C Y, Chen F C, Cheng T C, Lin H L, Lin T S, Yang W J, Lee Y I. 2018. Expression profiles of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxylase kinase genes in Phalaenopsis,implications for regulating the performance of crassulacean acid metabolism. Frontiers in plant science, 9:1587. doi: 10.3389/fpls.2018.01587 URL
[44]	Sapeta H, Costa J M, Lourenço T, Maroco J, van der Linde P, Oliveira M M. 2013. Drought stress response in Jatropha curcas:growth and physiology. Environmental and Experimental Botany, 85:76-84. doi: 10.1016/j.envexpbot.2012.08.012 URL
[45]	Shameer S, Baghalian K, Cheung C Y M, Ratcliffe R G, Sweetlove L J. 2018. Computational analysis of the productivity potential of CAM. Nature Plants, 4:165-171. doi: 10.1038/s41477-018-0112-2 pmid: 29483685
[46]	Shi Y, Zhang X, Chang X, Yan M, Zhao H, Qin Y, Wang H. 2021. Integrated analysis of DNA methylome and transcriptome reveals epigenetic regulation of CAM photosynthesis in pineapple. BMC Plant Biology, 21 (1):1-14. doi: 10.1186/s12870-020-02777-7 URL
[47]	Silvera K, Neubig K M, Whitten W M, Williams N H, Winter K, Cushman J C. 2010. Evolution along the crassulacean acid metabolism continuum. Functional Plant Biology, 37 (11):995-1010. doi: 10.1071/FP10084 URL
[48]	Stewart J R. 2015. Agave as a model CAM crop system for a warming and drying world. Frontiers in Plant Science, 6:684. doi: 10.3389/fpls.2015.00684 pmid: 26442005
[49]	Sussmilch F C, Brodribb T J, McAdam S A M. 2017. What are the evolutionary origins of stomatal responses to abscisic acid in land plants? Journal of Integrative Plant Biology, 59 (4):240-260. doi: 10.1111/jipb.12523
[50]	Wai C M, van Buren R. 2018. Circadian regulation of pineapple CAM photosynthesis.//Genetics and genomics of pineapple. Cham,Springer International Publishing:247-258.
[51]	Wai C M, van Buren R, Zhang J, Huang L, Miao W, Edger P P, Yim W C, Priest H D, Meyers B C, MocklerT, Smith J A C, Cushman J C, Ming R. 2017. Temporal and spatial transcriptomic and microRNA dynamics of CAM photosynthesis in pineapple. The Plant Journal, 92 (1):19-30. doi: 10.1111/tpj.13630 pmid: 28670834
[52]	Wai C M, Weise S E, Ozersky P, Mockler T C, Michael T P, van Buren R. 2019. Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album. PLOS Genetics, 15 (6):e1008209. doi: 10.1371/journal.pgen.1008209 URL
[53]	West-Eberhard M J, Smith J A C, Winter K. 2011. Photosynthesis,Reorganized. Science, 332 (6027):311. doi: 10.1126/science.1205336 pmid: 21493847
[54]	Winter K. 2019. Ecophysiology of constitutive and facultative CAM photosynthesis. Journal of Experimental Botany, 70 (22):6495-6508. doi: 10.1093/jxb/erz002 pmid: 30810162
[55]	Winter K, Garcia M, Holtum J A. 2009. Canopy CO₂ exchange of two neotropical tree species exhibiting constitutive and facultative CAM photosynthesis,Clusia rosea and Clusia cylindrica. Journal of Experimental Botany, 60 (11):3167-3177. doi: 10.1093/jxb/erp149 pmid: 19487388
[56]	Winter K, Holtum J A, Smith J A. 2015. Crassulacean acid metabolism:a continuous or discrete trait? New Phytol, 208 (1):73-78. doi: 10.1111/nph.13446 pmid: 25975197
[57]	Winter K, Sage R F, Edwards E J, Virgo A, Holtum J A. 2019. Facultative crassulacean acid metabolism in a C3-C4 intermediate. Journal of Experimental Botany, 70 (22):6571-6579. doi: 10.1093/jxb/erz085 pmid: 30820551
[58]	Winter K, Smith J A C. 2012. Crassulacean acid metabolism:biochemistry,ecophysiology and evolution. Springer Science & Business Media.
[59]	Xu S, Wang J, Guo Z, He Z, Shi S. 2020. Genomic convergence in the adaptation to extreme environments. Plant Communications, 1 (6):100117. doi: 10.1016/j.xplc.2020.100117 URL
[60]	Yang X, Cushman J C, Borland A M, Edwards E J, Wullschleger S D, Tuskan G A, Owen N A, Griffiths H, Smith J A C, de Paoli H C. 2015. A roadmap for research on crassulacean acid metabolism(CAM)to enhance sustainable food and bioenergy production in a hotter,drier world. New Phytologist, 207:491-504. doi: 10.1111/nph.13393 URL
[61]	Yang X, Hu R, Yin H, Jenkins J, Shu S, Tang H, Liu D, Weighill D A, Cheol Yim W, Ha J, HeydukK, Goodstein D M, Guo H B, Moseley R C, Fitzek E, Jawdy S, Zhang Z, Xie M, Hartwell J, Grimwood J, Abraham P E, Mewalal R, Beltrán J D, Boxall S F, Dever L V, Palla K J, Albion R, Garcia T, Mayer J A, Don Lim S, Man Wai C, Peluso P, Van Buren R, De Paoli H C, Borland A M, Guo H, Chen J G, Muchero W, Yin Y, Jacobson D A, Tschaplinski T J, Hettich R L, Ming R, Winter K, Leebens-Mack J H, Smith J A C, Cushman J C, Schmutz J, Tuskan G A. 2017. The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism. Nature Communications, 8 (1):1-15. doi: 10.1038/s41467-016-0009-6 URL
[62]	Yang X, Liu D, Tschaplinski T J, Tuskan G A. 2019. Comparative genomics can provide new insights into the evolutionary mechanisms and gene function in CAM plants. Journal of Experimental Botany, 70 (22):6539-6547. doi: 10.1093/jxb/erz408 pmid: 31616946
[63]	Yin H, Guo H-B, Weston D J, Borland A M, Ranjan P, Abraham P E, Jawdy S S, Wachira J, Tuskan G A, Tschaplinski T J, Wullschleger S D, Guo H, Hettich R L, Gross S M, Wang Z, Visel A, Yang X. 2018. Diel rewiring and positive selection of ancient plant proteins enabled evolution of CAM photosynthesis in Agave. BMC genomics, 19 (1):588. doi: 10.1186/s12864-018-4964-7 URL
[64]	Yuan G, Hassan M M, Liu D, Lim S D, Yim W C, Cushman J C, Markel K, Shih P M, Lu H, Weston D J. 2020. Biosystems design to accelerate C3-to-CAM progression. BioDesign Research, 2020.
[65]	Zhang G Q, Liu K W, Li Z, Lohaus R, Hsiao Y Y, Niu S C, Wang J Y, Lin Y C, Xu Q, Chen L J, Yoshida K, Fujiwara S, Wang Z W, Zhang Y Q, Mitsuda N, Wang M, Liu G H, Pecoraro L, Huang H X, Xiao X J, Lin M, Wu X Y, Wu W L, Chen Y Y, Chang S B, Sakamoto S, Ohme-Takagi M, Yagi M, Zeng S J, Shen C Y, Yeh C M, Luo Y B, Tsai W C, van de Peer Y, Liu Z J. 2017. The Apostasia genome and the evolution of orchids. Nature, 549 (7672):379-383. doi: 10.1038/nature23897 URL
[66]	Zhang G Q, Xu Q, Bian C, Tsai W C, Yeh C M, Liu K W, Yoshida K, Zhang L S, Chang S B, Chen F, Shi Y, Su Y Y, Zhang Y Q, Chen L J, Yin Y, Lin M, Huang H, Deng H, Wang Z W, Zhu S L, Zhao X, Deng C, Niu S C, Huang J, Wang M, Liu G H, Yang H J, Xiao X J, Hsiao Y Y, Wu W L, Chen Y Y, Mitsuda N, Ohme-Takagi M, Luo Y B, van de Peer Y, Liu Z J. 2016. The Dendrobium catenatum Lindl. genome sequence provides insights into polysaccharide synthase,floral development and adaptive evolution. Scientific Reports, 6:19029. doi: 10.1038/srep19029 URL
[67]	Zhang Y, Dong W, Zhao X, Song A, Guo K, Liu Z, Zhang L. 2019. Transcriptomic analysis of differentially expressed genes and alternative splicing events associated with crassulacean acid metabolism in orchids. Horticultural Plant Journal, 5 (6):268-280. doi: 10.1016/j.hpj.2019.12.001
[68]	Zheng L, Ceusters J, van Labeke M C. 2019. Light quality affects light harvesting and carbon sequestration during the diel cycle of crassulacean acid metabolism in Phalaenopsis. Photosynthesis Research, 141 (2):195-207. doi: 10.1007/s11120-019-00620-1 URL

植物 Plant	CAM类型 CAM type	组装水平 Assembly level	基因组/Mb Genome size	N50/kb	文献 Reference
玉吊钟Kalanchoe fedtschenkoi	组成型 Constitutive	支架水平 Scaffold	256	2 450（scaffold）	Yang et al.，2017
菠萝Ananas comosus	组成型Constitutive	染色体 Chromosome	382	11 800（scaffold）	Ming et al.，2015
红菠萝Ananas comosus var. bracteatus CB5	组成型Constitutive	染色体 Chromosome	513	427（contig）	Chen et al.，2019
玉米石Sedum album	组成型Constitutive	重叠群 Contig	302	93（contig）	Wai et al.，2019
麻疯树Jatropha curcas	兼性Facultative	支架水平 Scaffold	339	15 400（scaffold）	Ha et al.，2019
台湾蝴蝶兰Phalaenopsis aphrodite	组成型Constitutive	染色体 Chromosome	1 200	19 700（scaffold）	Chao et al.，2018
深圳拟兰Apostasia shenzhenica	组成型Constitutive	染色体 Chromosome	349	3 029（scaffold）	Zhang et al.，2017
小兰屿蝴蝶兰Phalaenopsis equestris	组成型Constitutive	支架水平 Scaffold	~980	359.1（scaffold）	Cai et al.，2015
黄石斛Dendrobium catenatum	兼性Facultative	支架水平 Scaffold	1 010	391（scaffold）	Zhang et al.，2016
铁皮石斛Dendrobium officinale	兼性Facultative	染色体 Chromosome	1 230	63 070（scaffold）	Niu et al.，2021

植物 Plant	CAM类型 CAM type	组装水平 Assembly level	基因组/Mb Genome size	N50/kb	文献 Reference
玉吊钟Kalanchoe fedtschenkoi	组成型 Constitutive	支架水平 Scaffold	256	2 450（scaffold）	Yang et al.，2017
菠萝Ananas comosus	组成型Constitutive	染色体 Chromosome	382	11 800（scaffold）	Ming et al.，2015
红菠萝Ananas comosus var. bracteatus CB5	组成型Constitutive	染色体 Chromosome	513	427（contig）	Chen et al.，2019
玉米石Sedum album	组成型Constitutive	重叠群 Contig	302	93（contig）	Wai et al.，2019
麻疯树Jatropha curcas	兼性Facultative	支架水平 Scaffold	339	15 400（scaffold）	Ha et al.，2019
台湾蝴蝶兰Phalaenopsis aphrodite	组成型Constitutive	染色体 Chromosome	1 200	19 700（scaffold）	Chao et al.，2018
深圳拟兰Apostasia shenzhenica	组成型Constitutive	染色体 Chromosome	349	3 029（scaffold）	Zhang et al.，2017
小兰屿蝴蝶兰Phalaenopsis equestris	组成型Constitutive	支架水平 Scaffold	~980	359.1（scaffold）	Cai et al.，2015
黄石斛Dendrobium catenatum	兼性Facultative	支架水平 Scaffold	1 010	391（scaffold）	Zhang et al.，2016
铁皮石斛Dendrobium officinale	兼性Facultative	染色体 Chromosome	1 230	63 070（scaffold）	Niu et al.，2021

植物（文献） Plant（Reference）	CAM类型 CAM type	转录因子 Transcription factor	转录因子家族 Transcription factor family	拟南芥同源基因 Arabidopsis homolog
冰叶日中花 Mesembryanthemum crystallinum （Amin et al.，2019）	兼性 Facultative	McERF74	AP2/ERF/CRF	AT1G53910
		McNAC29	NAC	AT1G69490
		McBLH1	HB/Homeodomain	AT2G35940
		McbZIP2	bZIP	AT2G18160
		McAGL8	MADS/AGAMOUS-LIKE8	AT5G60910
		McAP2-12	AP2/ERF	AT1G53910
		McbZIP44	bZIP	AT1G75390
		McHB7	HB/Homeodomain	AT2G46680
玉吊钟 Kalanchoe fedtschenkoi （Yang et al.，2017）	组成型 Constitutive	KfMYB59^*	MYB	AT5G59780
		KfLHY1	Homeodomain	AT1G01060
		KfbZIP29	bZIP	AT4G38900
		KfNF-YB3	NF-Ys	AT4G14540
		KfNAC83^*	NAC	AT4G13180
		KfAP2	AP2/ERF/CRF	AT4G11140
		KfCOL3	Zinc Finger CONSTANS-like 4	AT5G24930
		KfCOL5	Zinc Finger CONSTANS-like 5	AT5G67660
棱轴土人参 Talinum triangulare （Maleckova et al.，2019）	兼性 Facultative	TtOFP8	-	AT5G19650
		TtNF-YA9	NF-YA	AT3G20910
		TtNF-YB3	NF-YB	AT4G14540
		TtLBD21	LBD	AT3G11090
		TtHB-1	HD-ZIP	AT3G01470
		TtHSFA2	HSF	AT2G26150
		TtBBX15	CO-like	AT1G25440
		TtMP	ARF	AT1G19850
		-	Trihelix	AT2G44730
		-	-	AT4G00990
		TtHSFC1	HSF	AT3G24520

植物（文献） Plant（Reference）	CAM类型 CAM type	转录因子 Transcription factor	转录因子家族 Transcription factor family	拟南芥同源基因 Arabidopsis homolog
冰叶日中花 Mesembryanthemum crystallinum （Amin et al.，2019）	兼性 Facultative	McERF74	AP2/ERF/CRF	AT1G53910
		McNAC29	NAC	AT1G69490
		McBLH1	HB/Homeodomain	AT2G35940
		McbZIP2	bZIP	AT2G18160
		McAGL8	MADS/AGAMOUS-LIKE8	AT5G60910
		McAP2-12	AP2/ERF	AT1G53910
		McbZIP44	bZIP	AT1G75390
		McHB7	HB/Homeodomain	AT2G46680
玉吊钟 Kalanchoe fedtschenkoi （Yang et al.，2017）	组成型 Constitutive	KfMYB59^*	MYB	AT5G59780
		KfLHY1	Homeodomain	AT1G01060
		KfbZIP29	bZIP	AT4G38900
		KfNF-YB3	NF-Ys	AT4G14540
		KfNAC83^*	NAC	AT4G13180
		KfAP2	AP2/ERF/CRF	AT4G11140
		KfCOL3	Zinc Finger CONSTANS-like 4	AT5G24930
		KfCOL5	Zinc Finger CONSTANS-like 5	AT5G67660
棱轴土人参 Talinum triangulare （Maleckova et al.，2019）	兼性 Facultative	TtOFP8	-	AT5G19650
		TtNF-YA9	NF-YA	AT3G20910
		TtNF-YB3	NF-YB	AT4G14540
		TtLBD21	LBD	AT3G11090
		TtHB-1	HD-ZIP	AT3G01470
		TtHSFA2	HSF	AT2G26150
		TtBBX15	CO-like	AT1G25440
		TtMP	ARF	AT1G19850
		-	Trihelix	AT2G44730
		-	-	AT4G00990
		TtHSFC1	HSF	AT3G24520

景天酸代谢植物分子生物学研究进展及应用潜力

Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 68

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

[1]	王妍, 孙政, 冯珊, 袁心怡, 仲林林, 曾云流, 傅小鹏, 程运江, 包满珠, 张帆. 香石竹DcERF-1转录因子对切花衰老的负调控作用[J]. 园艺学报, 2022, 49(6): 1313-1326.
[2]	李琼, 李丽丽, 侯娟, 罗忍忍, 王瑞丹, 胡建斌, 黄松. 瓜类作物响应低温胁迫机制的研究进展[J]. 园艺学报, 2022, 49(6): 1382-1394.
[3]	苏江硕, 贾棣文, 王思悦, 张飞, 蒋甲福, 陈素梅, 房伟民, 陈发棣. 中国菊花遗传育种60年回顾与展望[J]. 园艺学报, 2022, 49(10): 2143-2162.
[4]	张婷婷, 薛婉钰, 刘娜, 陈书霞. 几种主要果菜类蔬菜果形遗传及其调控机制研究进展[J]. 园艺学报, 2022, 49(10): 2189-2204.
[5]	王云, 张镇武, 孙逊, 张绍铃. 植物自噬与病原菌互作研究进展[J]. 园艺学报, 2022, 49(10): 2205-2222.
[6]	杨博, 魏佳, 李坤峰, 王程亮, 倪隽蓓, 滕元文, 白松龄. PpyERF060-PpyABF3-PpyMADS71调控乙烯信号通路介导的梨芽休眠进程[J]. 园艺学报, 2022, 49(10): 2249-2262.
[7]	曾泽湘, 肖显梅, 谭小丽, 范中奇, 陈建业. 菜薹BrWRKY57的特性及其对BrPPH1和BrNCED3的调控[J]. 园艺学报, 2021, 48(3): 518-530.
[8]	李崇晖, 杨光穗, 张志群, 尹俊梅. 红掌R2R3-MYB转录因子基因AaMYB6调控花青素苷合成[J]. 园艺学报, 2021, 48(10): 1859-1872.
[9]	毛鹏鹏, 郑胤建, 杨其长, 许亚良, 王芳, 廖秋红, 刘晓英. 光质对十字花科蔬菜硫代葡萄糖苷调控分子机制研究进展[J]. 园艺学报, 2020, 47(9): 1633-1647.
[10]	刘兴旺, 翟许玲, 张亚琦, 尹帅, 冯钟萱, 任华中, . 黄瓜果实形态建成的遗传及分子基础研究进展[J]. 园艺学报, 2020, 47(9): 1793-1809.
[11]	徐小迪1,2，李博强1，秦国政1，陈彤1，张占全1，田世平1,2,*. 果实采后品质维持的分子基础与调控技术研究进展[J]. 园艺学报, 2020, 47(8): 1595-1609.
[12]	王军伟，黄科，黄英娟，毛舒香，柏艾梅，刘明月，吴秋云*. 十字花科蔬菜硫代葡萄糖苷合成相关转录因子调控研究进展[J]. 园艺学报, 2019, 46(9): 1752-1764.
[13]	芦旺1，席万鹏1,2,*. MADS-box转录因子在果实成熟及品质形成中的调控作用研究进展[J]. 园艺学报, 2018, 45(9): 1802-1812.
[14]	曹运琳，邢梦云，徐昌杰，李鲜*. 植物黄酮醇生物合成及其调控研究进展[J]. 园艺学报, 2018, 45(1): 177-192.
[15]	李青1,2，秦玉芝2，胡新喜2，王万兴1,，熊兴耀1,2,. 马铃薯耐盐性研究进展[J]. 园艺学报, 2017, 44(12): 2408-2424.

景天酸代谢植物分子生物学研究进展及应用潜力

Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 68

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: