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园艺学报 ›› 2023, Vol. 50 ›› Issue (5): 1063-1072.doi: 10.16420/j.issn.0513-353x.2022-0155

• 栽培·生理生化 • 上一篇    下一篇

苹果砧木‘冀砧2号’绞缢、环割压条生根效果及过程中IAA含量的变化

高美娜, 孙明飞, 朱杰, 井俊丽, 李佳, 周莎莎, 梁博文, 徐继忠, 李中勇()   

  1. 河北农业大学园艺学院,河北保定 071000
  • 收稿日期:2022-12-31 修回日期:2023-03-23 出版日期:2023-05-25 发布日期:2023-05-31
  • 通讯作者: *(E-mail:yylzy2014@163.com
  • 基金资助:
    财政部和农业农村部:国家现代苹果产业技术体系(CARS-27);河北省重点研发计划项目(20326802D);河北省重点研发计划项目(21326308D);河北省现代农业产业技术体系苹果创新团队项目(HBCT2021100409)

The Rooting Effect and Changes of IAA Content in Constriction,Girdling Treatment During Shoot Layering of Apple Rootstock‘Jizhen 2’

GAO Meina, SUN Mingfei, ZHU Jie, JING Junli, LI Jia, ZHOU Shasha, LIANG Bowen, XU Jizhong, LI Zhongyong()   

  1. College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071000,China
  • Received:2022-12-31 Revised:2023-03-23 Published:2023-05-25 Online:2023-05-31

摘要:

以苹果矮化砧木‘冀砧2号’为试材,研究绞缢、环割压条生根效果和IAA含量及相关指标变化,结果表明:绞缢处理的压条生根率为78.92%,显著高于环割和对照;压条生根过程中,绞缢处理的IAA含量显著高于环割和对照,35 d时出现最大值,为39.93 ng · g-1;不定根诱导期和形成期,绞缢处理的POD、PPO活性显著高于环割和对照,IAAO活性均低于环割和对照;不定根诱导期,绞缢处理的MdPAT1MdPIN1相对表达量逐渐上升,35 d时相对表达量最高,显著高于对照;25 d时MdYUCCA4相对表达量显著高于环割和对照,MdARF17的相对表达量则显著低于环割和对照。综上,绞缢可显著提高‘冀砧2号’砧木压条生根率和生根指数,生长素IAA对‘冀砧2号’不定根根原基的诱导和促进不定根伸长具有重要作用。

关键词: 苹果, 砧木, 压条生根, IAA

Abstract:

The changes of IAA content and other related indexes during shoot layering in apple dwarfing rootstock‘Jizhen 2’were determined. The results showed that:the rooting rate of constriction treatment was significantly higher than girdling and control,the highest rooting rate was 78.92%;During the shoot layering process,the IAA content of constriction treatment was significantly higher than that of girdling and control,and reached the maximum value of 39.93 ng · g-1 at 35 days;At adventitious roots induction and formation stage,the activities of POD and PPO of constriction treatment were significantly higher than those of girdling and the control,the IAAO activity of constriction treatment was lower than that of girdling and control;The upregulation of MdPAT1MdPIN1 during induction in constriction treatment were detected,and reached the highest level at 35 days,which was significantly higher than control;The relative expression of MdYUCCA4 was significantly higher in constriction treatment than that of girdling and control at 25 days and the expression of MdARF17 was relatively low in constriction treatment. In conclusion,The constriction treatment can significantly improve the rooting rate and rooting index of ‘Jizhen 2’rootstock. Auxin IAA plays an important role in inducing and promoting adventitious root elongation of‘Jizhen 2’.

Key words: apple, root stock, shoot layering, IAA

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