园艺学报 ›› 2022, Vol. 49 ›› Issue (2): 407-415.doi: 10.16420/j.issn.0513-353x.2021-0058

• 研究报告 • 上一篇    下一篇


武晓晓, 陈传武, 刘萍, 唐艳, 邓崇岭()   

  1. 广西特色作物研究院,广西柑橘育种与栽培工程技术研究中心,广西桂林 541004
  • 收稿日期:2021-05-20 修回日期:2021-12-17 出版日期:2022-02-25 发布日期:2022-02-28
  • 通讯作者: 邓崇岭
  • 基金资助:

Genetic Evolution and Taxonomic Status Analysis of Wild Citrus Resources Based on Resequencing in Guposhan Mountain in Guangxi Province

WU Xiaoxiao, CHEN Chuanwu, LIU Ping, TANG Yan, DENG Chongling()   

  1. Guangxi Citrus Breeding and Cultivation Research Center of Engineering Technology,Guangxi Academy of Specialty Crops,Guilin,Guangxi 541004,China
  • Received:2021-05-20 Revised:2021-12-17 Online:2022-02-25 Published:2022-02-28
  • Contact: DENG Chongling


对4个广西姑婆山野生柑橘、1个枳、1个金柑以及14个其他柑橘进行全基因组重测序,每个样本测序数据量约为16 Gb,平均测序深度为54×。通过基因组比对、SNP检测、PCA主成分分析、邻接法构建系统发育进化树,分析广西贺州姑婆山野生柑橘资源遗传多样性、群体结构及分类地位。从20份样品中,共检测出6 782 158个高质量SNP位点。基于这些SNP位点,根据PCA主成分分析和系统进化树分析结果,可将20份样本分为3类:广西姑婆山野柑与莽山野柑聚为一类,金柑与其他柑橘聚为一类,枳聚为单独一类。该研究表明姑婆山野生柑橘资源与莽山野柑一样具有原始性和独特性,应该为独立的属或者柑橘属中独立的种。

关键词: 柑橘, 基因组, 进化, 多样性


Analyzing the genetic evolution and taxonomic status of wild citrus resources in Guposhan Mountain in Guangxi Province,provides a theoretical basis for citrus germplasm innovation and genetic improvement,and also provides a basis for in-depth exploration of genes related to excellent traits in the future. Whole genome sequencing was performed on four wild citrus varieties from Guposhan Mountain in Guangxi,one Poncirus trifoliata,one kumquat,and 14 other citrus. The amount of sequencing data for each sample was about 16 Gb,and the average sequencing depth was 54×. Through short read mapping,SNP detection,PCA principal component analysis,and neighbor joining method to construct a phylogenetic tree,reveal its genetic diversity and population genetic structure. From 20 samples,6 782 158 high-quality SNP loci were detected. Based on these SNP sites,and according to the PCA principal component analysis and phylogenetic tree analysis results,20 samples can be divided into three categories: wild citrus in Guposhan and C. mangshanensis are clustered together,and kumquat and other citrus are clustered together. Poncirus trifoliata is a separate category. The study analyzed the genetic diversity and population evolution of wild citrus resources in Guposhan Mountain from the whole genome level,and showed that wild citrus resources in Guposhan Mountain are primitive and unique like C. mangshanensis. They are supposed to be an independent and true species within the genus citrus,or it is a separate genus other than Poncirus,Fortunella Swing and Citrus L.

Key words: citrus, genome, evolution, diversity