园艺学报 ›› 2019, Vol. 46 ›› Issue (11): 2109-2118.doi: 10.16420/j.issn.0513-353x.2018-1089

• 研究论文 • 上一篇    下一篇


李贝贝1,*,张 恒1,2,*,姜建福1,张 颖1,樊秀彩1,房经贵2,**,刘崇怀1,**   

  1. 1中国农业科学院郑州果树研究所,郑州 450009;2南京农业大学园艺学院,南京 210095
  • 出版日期:2019-11-25 发布日期:2019-11-25
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-29-yz-1);中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP- 2018-ZFRI)

Analysis of Genetic Diversity of Grape Germplasms Using SLAF-seq Technology

LI Beibei1,*,ZHANG Heng1,2,*,JIANG Jianfu1,ZHANG Ying1,FAN Xiucai1,FANG Jinggui2,**,and LIU Chonghuai1,**   

  1. 1Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;2College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2019-11-25 Published:2019-11-25

摘要: 利用简化基因组测序技术(SLAF-seq)对304份葡萄种质进行测序,最终获得466 618个SLAF标签,其中多态性SLAF标签392 374个。通过序列分析,获得481 192个有效单核苷酸(SNP)多态标记,并基于这些SNP分析和构建了304个葡萄种质的群体结构和系统发生树。结果表明,SLAF-seq技术能高效、低廉地开发出大量可用于群体遗传分析的SNP标记。通过构建进化树分析发现,欧亚种、欧美杂种及野生种葡萄彼此分开,其中姊妹系品种及具有亲子代关系的品种各自聚在一起,中国葡萄地方品种与国外古老葡萄品种的亲缘关系最近,且起源及遗传背景不详的古老葡萄品种与野生种的亲缘关系较改良品种更近。该结果可为后期进一步研究葡萄的起源进化提供参考,同时为构建SNP指纹数据库提供了基础数据。

关键词: 葡萄, SLAF-seq, SNP, 遗传多样性

Abstract: In this study,304 Vitis accessions were used as experimental materials for sequencing by specific-locus amplified fragment sequencing(SLAF-seq). In total,466 618 high quality SLAF tags were obtained,including 392 374 polymorphic SLAF tags. A total of 481 192 single nucleotide polymorphisms (SNPs)were obtained by sequence analysis. The phylogenetic relationship and population structure of 304 Vitis accessions were analyzed based on the SNP dataset,which suggests that SLAF-seq can be used to develop a large number of SNPs for population genetic analysis,efficiently and cheaply. The clustering results showed that V. vinifera,Euro-America hybrid and wild species were distinguished from each other,among which sister lines and varieties with parent-child relationship were clustered together,respectively. In addition,the Chinese grape landraces have a close relationship with the ancient Eurasian grape varieties,and the ancient grape varieties with with unknown origin and genetic background are closer to the wild species than the improved varieties. These results provide references for further study of the origin andevolution of grapes,and provide empirical data for the establishment of SNP fingerprint database.

Key words: grape, SLAF-seq, SNP, genetic diversity