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园艺学报 ›› 2015, Vol. 42 ›› Issue (4): 759-768.doi: 10.16420/j.issn.0513-353x.2014-1030

• 研究报告 • 上一篇    下一篇

新疆野苹果叶形性状变异及其与SSR标记关联分析

左力辉,张文林,邱 彤,张 军,杨敏生   

  1. 河北农业大学林学院,林木种质资源与森林保护重点实验室,河北保定 071000
  • 出版日期:2015-04-25 发布日期:2015-04-25
  • 基金资助:
    国家林业局林业公益性行业专项(201104039);河北农业大学青年科学基金项目(QJ201212)

Malus sieversii Leaf Traits Variation and Correlation Analysis with SSR Markers

ZUO Li-hui,ZHANG Wen-lin,QIU Tong,ZHANG Jun,and YANG Min-sheng*   

  1. The Key Laboratory of Germplasm Resources of Forest Trees and Forests Protection,College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000,China
  • Online:2015-04-25 Published:2015-04-25

摘要: 以从新疆天山地区新疆野苹果600个无性系中筛选得到的18个优系为研究对象,调查其叶形性状变异,并进行了SSR分子标记分析,探究叶形性状与SSR标记间的相关关系。结果表明,调查的15个叶形性状在无性系间均存在极显著差异;平均变异系数最大的为叶尖角α(达到25.39%),叶形指数L1/L3最小(仅为10.31%);叶片长的重复力最大(达到0.967),叶形指数L1/L3最小(仅为0.495)。以15个叶片性状为依据,可将各无性系完全区分开,并分为3类,各无性系间的遗传距离变化在1.293 ~ 7.235间。用均匀分布在17个染色体连锁群上的30对多态性SSR引物对18个无性系进行聚类分析,可将各无性系完全区分开,并可分为4类,各无性系间的遗传距离为0.089 ~ 0.689,平均遗传距离为0.433。所有叶形性状聚类结果与所有SSR标记聚类结果不相关。以单一叶形性状聚类结果与经过筛选的部分SSR标记聚类结果进行相关分析,绝大部分达到极显著相关,且大部分引物组合表现为累积效应,表明叶形性状与部分SSR位点具有紧密关联。

关键词: 新疆野苹果, 表型性状, SSR, 相关分析

Abstract: Investigated leaf shape characters and SSR molecular marker analysis of the 18 Xinjiang wild apple[Malus sieversii(Ledeb.)Roem.] clones which were selected from 600 clones of Tianshan area to explore the correlation between leaf traits and SSR markers. Results indicate that all the 15 leaf traits were extremely significant differences between the clones. The maximum average coefficient of variation is shape of leaf tip(α),with the value of 25.39% while the shape index of leaf L1/L3 was the minimum with the variation value of 10.31%. Repeatability of the leaf length having the maximum value of 0.967 while the shape index of leaf L1/L3 was the minimum with the repeatability value of 0.495. With 15 blade shape traits clustering,all clones cluster into three groups and each clone can be completely separate withgenetic distance variation changing between 1.293 to 7.235. The genomic polymorphism of clones were analyzed using 30 pairs of SSR primers. The clustering analysis of 30 SSR markers with uniform distribution in 17 chromosome chain groups showed all clones cluster into four groups and each clone can be completely separate with genetic distance variation changing from 0.089 to 0.689 and the average was 0.433. The correlation coefficient of the clustering analysis of leaf traits and SSR was only 0.105. Correlation analysis of single leaf shape traits and the filtered portion of SSR markers clustering results showed that most of the assembly achieve significant correlation and most of the loci were closely related.

Key words: Malus sieversii, phenotypic character, SSR, correlation analysis

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