https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2021, Vol. 48 ›› Issue (7): 1397-1408.doi: 10.16420/j.issn.0513-353x.2021-0203

• 研究论文 • 上一篇    下一篇

利用SSRseq分子标记的大蒜种质资源遗传多样性研究

赵青, 都真真, 李锡香, 宋江萍, 张晓辉, 阳文龙, 贾会霞, 王海平*()   

  1. 中国农业科学院蔬菜花卉研究所,农业农村部园艺作物生物学与种质创制重点实验室,北京 100081
  • 收稿日期:2021-02-23 修回日期:2021-04-07 出版日期:2021-07-25 发布日期:2021-08-10
  • 通讯作者: 王海平 E-mail:wanghaiping@caas.cn
  • 基金资助:
    国家自然科学基金项目(31872946);国家重点研发计划项目(2016YFD0100204);国家现代农业产业技术体系建设专项资金项目(CARS-24-A-01);中国农业科学院创新工程项目(CAAS-ASTIP-2017-IVFCAAS);中央级公益性科研院所基本科研业务费专项(IVF-BRF2018001);农业农村部作物种质资源保护项目;农业农村部作物种质资源安全保存项目;国家园艺种质北京蔬菜分库运行服务项目(NHGRC2021-NH01);国家作物种质资源数据中心观测监测任务项目(ZX01NK-03)

Genetic Diversity of Garlic Germplasm Resources Based on SSRseq Molecular Markers

ZHAO Qing, DU Zhenzhen, LI Xixiang, SONG Jiangping, ZHANG Xiaohui, YANG Wenlong, JIA Huixia, WANG Haiping*()   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and Germplasm Creation in the Ministry of Agriculture and Rural Affairs,Beijing 100081,China
  • Received:2021-02-23 Revised:2021-04-07 Online:2021-07-25 Published:2021-08-10
  • Contact: WANG Haiping E-mail:wanghaiping@caas.cn

摘要:

利用筛选出的24对SSRseq引物对来自世界各地的676份大蒜种质进行遗传多样性、主坐标和群体遗传结构分析。结果表明:24对SSRseq标记在676份种质中共检测到124个多态性位点,平均等位位点数为5.17个,Nei’s多样性指数平均为1.77,Shannon’s 信息指数平均为0.62,平均观测杂合度和期望杂合度分别为0.33和0.34。用邻近法将676份资源划分为4类,其中第Ⅰ类包括以来源于亚洲中高纬度为主的186份种质,第Ⅱ类包括以来源于欧洲、中亚和北美洲为主的205份种质,第Ⅲ类包括以亚洲半抽薹类型的资源为主的132份种质,第Ⅳ类包括以来源于亚洲低纬度为主的153份种质。主坐标分析表明,亚洲低纬度地区的种质聚集在第Ⅰ类与第Ⅲ类,亚洲中高纬地区、欧洲、北美洲地区的种质聚集在第Ⅱ类。群体遗传结构分析将种质资源划分为4类,与邻近法聚类分析相似,以来自欧洲、中亚、北美洲为主的种质资源群体基因多样性和Shannon’s多样性信息指数最大,分别为0.3213与0.5933。用邻近法聚类分析与群体遗传结构分析均将382份中国资源划分为4类,分别以华北、华南、蒙新、西南地区种质为主。主坐标分析表明,中国华北、华南地区的种质分布在PC1的两端,表现出较远的亲缘关系。研究结果将为后期大蒜资源的深入评价和品种选育提供重要参考。

关键词: 大蒜, SSRseq, 群体结构, 遗传多样性

Abstract:

In this study,we revealed the genetic diversity,principal coordinate analysis and population structure analysis of 676 garlic accessions from all over the word by using 24 pairs of selected primers. The results indicate that a total of 124 polymorphic sites were detected,with an average of 5.17 alleles,an average of 1.77 for Nei's diversity index and 0.62 for Shannon information index. The mean values of observed and expected heterozygosity were 0.33 and 0.34,respectively. The method of neighbor-joining clustered all the accessions into four groups,group Ⅰ included 186 accessions,most of which were derived from middle and high latitudes of Asia,group Ⅱ included 205 accessions,most of which were derived from Europe,Central Asia and North America,group Ⅲ included 132 accessions,most of which were half-bolting types from Asia,group Ⅳ included 153 accessions,most of which were derived from lower latitudes of Asia. The garlic population was divided into three groups by principal coordinate analysis,accessions from lower latitude of Asia gathered in group Ⅰ and group,accessions from middle and high latitudes of Asia,Europe,North America area gathered in group Ⅱ. Population structure analysis divided the germplasm into four groups,which were similar to cluster analysis,there was gene exchange among different garlic groups. The genetic diversity and Shannon's diversity information index of germplasm resources from Europe,Central Asia and North America were the highest,which were 0.3213 and 0.5933,respectively,indicating that this population had rich genetic diversity. The method of neighbor-joining cluster analysis and population genetic structure analysis divided 382 Chinese resources into four groups,mainly from North China,South China,Mongol Xinjiang and Southwest China,respectively. The germplasm of North and South China was distributed at both ends of PC1 by principal coordinate analysis,showing a distant phylogenetic relationship. The results can provide scientific basis for the identification and evaluation of garlic germplasm and the breeding of superior garlic varieties.

Key words: garlic, SSRseq, population structure, genetic diversity

中图分类号: