Establishment and Application of a Real-time Fluorescent Quantitative RT-PCR for Detection of Grapevine fabavirus

doi:10.16420/j.issn.0513-353x.2019-0216

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (1): 187-194.doi: 10.16420/j.issn.0513-353x.2019-0216

• New Technologies and New Methods • Previous Articles Next Articles

Establishment and Application of a Real-time Fluorescent Quantitative RT-PCR for Detection of Grapevine fabavirus

ZHANG Mengyan，ZHANG Zunping，REN Fang，HU Guojun，FAN Xudong*，and DONG Yafeng*

National Center for Eliminating Viruses from Deciduous Fruit Tree，Research Institute of Pomology，Chinese Academy of Agriculture Sciences，Xingcheng，Liaoning 125100，China

Online:2020-01-25 Published:2020-01-25

Abstract

Abstract: A SYBR GreenⅠRT-qPCR method for GFabV detection was established，an excellent linear correlation（0.998）and amplification efficiency（103.8%）were obtained from standard curve. The sensitivity of the method was 1 000 times higher than conventional RT-PCR，and had a strong specificity. The established RT-qPCR and conventional RT-PCR were used to detect GFabV in 316 grapevine samples from different development stages and different positions，and the results showed that the detection rate of RT-qPCR（96.8%）was apparently higher than conventional RT-PCR（70.8%）. The detection rate of dormant branches using RT-qPCR was 100%，and for other positions detection were all above 92%，which were apparently higher than conventional RT-PCR（42%–97%）. The detection rates using RT-qPCR（85%–95%）were higher than conventional RT-PCR（70%–90%）in these samples from different development stages. Compared with conventional RT-PCR，the newly established RT-qPCR has obvious superiority in detection of various samples.

Key words: grape, Grapevine fabavirus, RT-qPCR, RT-PCR

CLC Number:

S 663.1

ZHANG Mengyan，ZHANG Zunping，REN Fang，HU Guojun，FAN Xudong*，and DONG Yafeng*. Establishment and Application of a Real-time Fluorescent Quantitative RT-PCR for Detection of Grapevine fabavirus[J]. ACTA HORTICULTURAE SINICA, 2020, 47(1): 187-194.

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Establishment and Application of a Real-time Fluorescent Quantitative RT-PCR for Detection of Grapevine fabavirus

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