Cloning and Prokaryotic Expression of Expansin cDNA in Persimmon Fruits

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (7): 1139-1146.

• 研究报告 • Previous Articles Next Articles

Cloning and Prokaryotic Expression of Expansin cDNA in Persimmon Fruits

CHANG Xiao-xiao，RAO Jing-ping*，and LIU Le

（College of Horticulture，Northwest A & F University，Yangling，Shaanxi 712100，China）

Received:2010-03-12 Revised:2010-05-19 Online:2010-07-25 Published:2010-07-25
Contact: RAO Jing-ping

Abstract

Abstract: Using the total RNA from different stages of persimmon（Diospyros kaki L.‘Fuping Jianshi’）fruit as the template，five expansin cDNA：CDK-Exp3（1 168 bp）from mature stage，CDK-Exp4（1 120 bp）and CDK-Exp5（1 018 bp）from colour-changed stage，CDK-Exp6（1 129 bp）and CDK-Exp7（1 121 bp）from fruit expanding stage were isolated by RT-PCR and RACE. The open reading frame of the five genes was 765 bp and encoded a protein of 254 amino acid residues. The CDK-Exp3 was cloned into pET-32a（+）vector to construct recombination prokaryotic expression vector pET-CDK-Exp3，and which was transformed to E.coli BL21. Recombinant protein about 43 kD was expressed in pET-CDK-
Exp3 system and separated by SDS-PAGE electrophoresis.

Key words: persimmon, fruit, expansin, gene, cDNA clone, prokaryotic expression

CLC Number:

S 665.2

CHANG Xiao-xiao;RAO Jing-ping;and LIU Le. Cloning and Prokaryotic Expression of Expansin cDNA in Persimmon Fruits [J]. ACTA HORTICULTURAE SINICA, 2010, 37(7): 1139-1146.

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Cloning and Prokaryotic Expression of Expansin cDNA in Persimmon Fruits

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