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园艺学报 ›› 2020, Vol. 47 ›› Issue (6): 1126-1140.doi: 10.16420/j.issn.0513-353x.2019-0398

• 研究论文 • 上一篇    下一篇

石蒜鳞茎膨大过程中内源激素相关基因的差异表达研究

许俊旭1,李青竹1,李 叶2,杨柳燕1,李 心1,王 桢1,张永春1,*   

  1. 1上海市农业科学院林木果树研究所,上海 201403;2上海市金山区廊下镇农业技术推广服务站,上海 201516
  • 出版日期:2020-06-25 发布日期:2020-06-25
  • 基金资助:
    上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-1-6号];上海市市级农口青年人才成长计划项目[沪农青字(2018)第1-10号]

Differential Expression of Genes Related to Endogenous Hormone During Bulb Development in Lycoris radiata

XU Junxu1,LI Qingzhu1,LI Ye2,YANG Liuyan1,LI Xin1,WANG Zhen1,and ZHANG Yongchun1,*   

  1. 1Forestry and Pomology Research Institute,Shanghai Academy of Agriculture Sciences,Shanghai 201403,China;2Agricultural Technology Extension Service Station,Langxia Town,Jinshan District,Shanghai 201516,China
  • Online:2020-06-25 Published:2020-06-25

摘要: 石蒜(Lycoris radiata)鳞茎自然膨大过程很慢,严重制约其商业化生产的发展,研究鳞茎膨大过程中内源激素的变化规律,能够为利用外施生长调节剂调控石蒜鳞茎的膨大提供理论依据。本研究通过构建同一遗传背景下的石蒜鳞茎膨大过程材料,研究鳞茎膨大过程中4种内源激素:生长素(IAA)、细胞分裂素(CK)、赤霉素(GA)以及脱落酸(ABA)含量的变化,并通过转录组测序的方式,研究此过程中内源激素合成及信号转导基因表达的变化。结果表明:内源GA能够负调控石蒜鳞茎的膨大,而内源IAA和CK正向调控,但IAA的作用时期可能更多集中于鳞茎膨大早期,且可能通过调控CK的合成及信号转导过程来发挥作用,内源ABA可能在石蒜鳞茎膨大的调控过程中作用不大。另外,还发现茉莉酸(JA)和水杨酸(SA)相关基因在石蒜鳞茎膨大过程中表达上调,暗示JA和SA的调控作用可能与石蒜鳞茎的膨大呈正相关。

关键词: 石蒜, 鳞茎膨大, 转录组, IAA, GA, ABA

Abstract: The slow growth of Lycoris radiata bulbs under natural conditions severely restricts the development of the Lycoris industry. Understanding changes in endogenous hormone regulation during bulb development could provide a theoretical basis for accelerating bulb enlargement through the application of exogenous hormone. In this study,four groups of developing Lycoris radiata bulbs with the same genetic background were developed and used to investigate changes in the content of four endogenous hormones:auxin,cytokinin,gibberellin,and abscisic acid. The expression patterns of the genes involved in hormone biosynthesis and signal transduction were also examined through transcriptome analysis. The results showed that endogenous gibberellin could negatively regulate the development of Lycoris bulbs,while endogenous auxin and cytokinin positively regulated development. Auxin mainly affected the early stage of bulb development and may act by regulating gene expression patterns related to cytokinin synthesis and signal transduction. The results also suggested that endogenous abscisic acid had little effect on the regulation of bulb development in Lycoris radiata. Finally,the study showed that genes related to jasmonic acid(JA)and salicylic acid(SA)were up-regulated during bulb development,implying that the regulation of JA and SA may be positively correlated with bulb development of Lycoris radiata.

Key words: Lycoris radiata, bulb development, transcriptome analysis, IAA, GA, ABA

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