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园艺学报 ›› 2018, Vol. 45 ›› Issue (10): 2037-2044.doi: 10.16420/j.issn.0513-353x.2018-0143

• 研究报告 • 上一篇    下一篇

一种新的柑橘采后病害病原的鉴定

徐国亮1,赵飞飞1,王清廉2,王世玲1,贾 瑾1,陈艳玲2,黄传宏3,刘永忠2,彭抒昂2,王国平1,洪 霓1,丁 芳1,2,*   

  1. 1华中农业大学植物科学技术学院,湖北省作物病虫害监测与安全控制重点实验室,武汉430070;2华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉430070;3湖北省宜昌市植保站,湖北宜昌443003
  • 出版日期:2018-10-25 发布日期:2018-10-25
  • 基金资助:

    国家重点研发计划项目(2017YFD0202000);中央高校基本科研业务费专项(2662016PY099);国家现代农业产业技术体系建设专项资金项目(CARS-26)

Identification of a New Pathogen of Citrus Postharvest Disease

XU Guoliang1,Zhao Feifei1,WANG Qinglian2,WANG Shiling1,JIA Jin1,CHEN Yanling2,HUANG Chuanhong3,LIU Yongzhong2,PENG Shu’ang2,WANG Guoping1,HONG Ni1,and DING Fang1,2,*   

  1. 1College of Plant Science and Technology,Hubei Key Laboratory of Plant Pathology,Huazhong Agricultural University,Wuhan 430070,China;2Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticultrue & Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;3Yichang Plant Protection Station,Yichang,Hubei 443003,China
  • Online:2018-10-25 Published:2018-10-25

摘要:

从疑似青霉、绿霉症状的甜橙(Citrus sinensis)果实样品中分离得到一株新的致病菌株Pu. XHz。该菌株在PDA培养基上形成白色圆形菌落,产生大量蓝灰色分生孢子,培养后期有液滴渗出;在察氏培养基(Czapek–Dox Medium,CA)上菌落呈烟花绽放状;在察氏酵母膏琼脂培养基(Czapek Yeast Autolysate agar,CYA)上菌落呈同心圆状;在25%甘油硝酸盐琼脂培养基(25% Glycerol nitrate agar,G25N)上白色菌丝体发达,不产生分生孢子。分生孢子大小为(4.48 ~ 8.37)μm ×(2.65 ~ 5.24)μm,大部分为长椭圆形。致病性测定结果表明,在金钱橘、茂谷柑和甜橙果实上接种3 d左右开始出现水渍状病斑并逐渐扩大,接种12 d左右在病斑中央产生许多特征性晶须状霉层(Whisker mold),为病原菌的孢梗束,其顶端产生大量蓝灰色分生孢子。ITS序列分析表明,Pu. XHz与日本菌株Penicillium ulaiense SU-9分离物同源性为99%,证实了P. ulaiense是导致柑橘果实采后霉状腐烂的病原菌,为进一步研究由其引起的发病规律奠定了基础。

关键词: 柑橘, 果实腐烂, 病原鉴定, 乌来青霉

Abstract:

A new pathogenic strain of Pu. XHz from Citrus sinensis fruit showing symptoms like blue-green mold was identified. Pu. XHz formed white round colonies on PDA medium. Large numbers of blue-gray spores and exudative droplets appeared in the late stage. On CA medium,it showed fireworks-shaped radial colonies. Meanwhile,the colonies of Pu. XHz formed on CYA medium looked like concentric circles. When cultured on G25N medium,white mycelium colonies developed well but did not produce conidia. The size of oval-shaped Pu. XHz conidia was ranging from 4.48–8.37 μm to 2.65–5.24 μm. Pathogenicity test showed,water-soaked ring spots formed 3 days post inoculation(dpi)on Fortunella margarit,Murcott and Citrus sinensis Osbeck‘Newhall’. In about 10 dpi,large quantities of blue-gray conidia and conidiophore with typical character of whisker molds formed in the site of infected lesions. Further analysis by ITS sequencing verified Pu. XHz was a new strain of P. ulaiense. It showed 99% homology with that of P. ulaiense stain SU-9 deposited in GenBank. Our present study verified that Pu. XHz was the causal agent of citrus whisker mold,which laid a basic foundation for further study on the pathogenicity of P. ulaiense.

Key words: citrus, fruit rot, pathogen identification, Penicillium ulaiense

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