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园艺学报 ›› 2018, Vol. 45 ›› Issue (8): 1429-1440.doi: 10.16420/j.issn.0513-353x.2017-0653

• 研究论文 •    下一篇

MdMYB10对苹果果皮苯丙氨酸代谢的影响

张 波1,曲 东2,杨惠娟1,杨亚洲1,王 帆1,朱珍珍1,赵政阳1,*   

  1. 1西北农林科技大学园艺学院,陕西省苹果工程技术研究中心,陕西杨凌 712100;2陕西理工大学生物科学与工程学院,陕西省资源生物重点实验室,陕西汉中 723000
  • 出版日期:2018-08-25 发布日期:2018-08-25

Effects of MdMYB10 Gene on Phenylalanine Metabolism of Apple Peel

ZHANG Bo1,QU Dong2,YANG Huijuan1,YANG Yazhou1,WANG Fan1,ZHU Zhenzhen1,and ZHAO Zhengyang1,*   

  1. 1College of Horticulture,Northwest A & F University,Apple E & T Research Centre of Shaanxi,Yangling,Shaanxi 712100,China;2School of Biological Science and Engineering,Shaanxi University of Technology,Shaanxi Key Laboratory Bio-resources,Hanzhong,Shaanxi 723000,China
  • Online:2018-08-25 Published:2018-08-25

摘要: MdMYB10对苹果果皮花青苷合成起重要作用,但对苯丙氨酸代谢途径中其他酚类物质合成是否起作用尚不清楚。从‘粉红女士’苹果(Malus ? domestica‘Pink Lady’)果皮中克隆了MdMYB10,分别构建其过表达载体pCAMBIA2301-MdMYB10和沉默载体pTRV2-MdMYB10,在套袋苹果果皮下注射农杆菌诱导MdMYB10过表达和沉默,分析MdMYB10对解袋后苹果果皮苯丙氨酸代谢的影响。结果表明,在过表达MdMYB10的果皮组织中,MdMYB10表达量上调了19.62倍,MdPAL、MdCHS、MdF3H、MdDFR、MdANS、MdUFGT、MdFLS、MdANR均有不同程度的上调,只有MdLAR无显著变化;沉默MdMYB10的果皮组织中,MdMYB10表达量降低了87%;MdCHS、MdF3H、MdDFR、MdANS和MdUFGT均有不同程度的下调,其中MdANS和MdUFGT下调最多,分别下调83%和82%。在过表达MdMYB10的果皮中,花青苷含量升高了3.05倍,槲皮素–3–O–半乳糖苷、表儿茶素、原花青素B2、绿原酸、对香豆酸含量均有不同程度升高,根皮苷含量无明显变化;在沉默MdMYB10的果皮中,花青苷含量降低了83%,槲皮素–3–O–半乳糖苷和表儿茶素含量分别降低58%和52%,原花青素B2、根皮苷含量无明显变化,而绿原酸含量升高了24%,对香豆酸含量升高了21%。可见,MdMYB10不仅调控苹果果皮中花青苷合成,同时还调控苯丙氨酸代谢中绿原酸、对香豆酸、槲皮素–3–O–半乳糖苷、表儿茶素和原花青素B2等物质的合成,但对根皮苷的形成作用不明显。

关键词: 苹果, MdMYB10, 过表达, 病毒诱导的基因沉默, 苯丙氨酸代谢

Abstract: MdMYB10 gene plays crucial roles on anthocyanin biosynthesis in apple fruits(Malus × domestica). However,the effects of MdMYB10 on other polyphenols biosynthesis in phenylalanine metabolism pathway are still unclear. In this study,MdMYB10 was cloned from the peel of‘Pink Lady’apple fruit. The overexpression vector pCAMBIA2301-MdMYB10 and interference vector pTRV-MdMYB10 were constructed respectively to verify the function of MdMYB10. In order to analyze the effect of MdMYB10 on phenylalanine metabolism in the peel of unbagged apple fruit,the overexpression and silence of MdMYB10 was induced by Agrobacterium tumefaciens injection. The results indicated that in the MdMYB10 overexpression apple fruit peel,MdMYB10 expression level was raised 19.62 times,and the expressions of MdPAL,MdCHS,MdF3H,MdDFR,MdANS,MdUFGT,MdFLS and MdANR were up-regulated with varying degrees,but no significant change was found for the MdLAR expression. In the MdMYB10 silenced apple fruit peel,the expression of MdMYB10 was down-regulated by 87%,and the expressions of MdCHS,MdF3H,MdDFR,MdANS and MdUFGT were suppressed in different degrees. Among them,the expression of MdANS and MdUFGT decreased most,with 83% and 82%,respectively. In addition,anthocyanins accumulation in the peel of apple fruits with overexpression vector increased 3.05 times. The content of quercetin-3-O-galactoside,epicatechin,procyanidins B2,chlorogenic acid and coumaric acid increased with varying degrees,and phlorizins concentration did not show significant change. In MdMYB10 silenced apple peel,anthocyanins accumulation decreased by 83%,and quercetin-3-O-galactoside and epicatechin content decreased by 58% and 52%,respectively. Procyanidins B2 and phlorizin concentration did not show significant change. By contrast,chlorogenic acid and coumaric acid content increased by 24% and 21%,respectively. Therefore,MdMYB10 gene not only regulates anthocyanin biosynthesis in apple fruit peel,but also regulates the chlorogenic acid,coumaric acid,quercetin-3-O-galactoside,epicatechin and procyanidins B2 biosynthesis in phenylalanine metabolism. However,no obvious effect was shown on phlorizin biosynthesis.

Key words: apple, MdMYB10, overexpression, VIGS, phenylalanine metabolism

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