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• 研究报告 •

### 松潘乌头嫩茎高效再生体系的建立

1. 1甘肃林业职业技术学院园林工程系，甘肃天水 741020；2甘肃神龙戎发药业股份有限公司，兰州 730070
• 出版日期:2015-07-25 发布日期:2015-07-25
• 基金资助:

兰州市科技创新人才团队培育计划项目（2011-1-140）

### The Establishment of the Efficient Regeneration System of the Tender Stems of Aconitum sungpanense

LEI Ying, REN Yi-Jie, SHEN Xiao-Yan

1. 1Department of Gardening Engineering，Gansu Forestry Technological College，Tianshui，Gansu 741020，China； 2Gansu Shenlongrongfa Pharmaceutical Co.，LTD，Lanzhou 730070，China
• Online:2015-07-25 Published:2015-07-25

Abstract: The tender stems of Aconitum sungpanense were used as the explants to make a study of the adventitious bud induction and the plantlet regeneration. The results are as follows：The tender stems with the length of 5–10 cm are the ideal material for the explant culture. When the adventitious buds are inducted by the tender stems，the medium of MS + 6-BA 4.0 mg ? L-1 + NAA 1.0 mg ? L-1 is beneficial for the differentiation and the differentiation rate reaches 94.8%，besides，the number of the average bud is 4.2. The medium of MS + 6-BA 3.0 mg ? L-1 + IBA 0.3 mg ? L-1 is suitable for the propagation of buds. Meanwhile，to make the control of the vitrification of seedlings，it is effective to add Benzylpenicillin Potassium with 5 mg ? L-1 and GA3 0.3 mg ? L-1 to the original propagation medium and it is ideal to adopt sucrose with 50 g ? L-1 and agar with 7 g ? L-1 so that buds can be multiplied by 7.26 times and the height of seedlings can rise to about 4 cm as well. The optimum medium for rooting is 1/2MS + IAA 0.3 mg ? L-1 and the average number of rooting is approximately 10，besides，the rooting rate increases to over 96%. The seedlings transplanted to the matrix with forest soil and grass carbon（the ratio of 1︰1）grow vigorously and the survival rate reaches over 90%.