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园艺学报 ›› 2020, Vol. 47 ›› Issue (7): 1301-1311.doi: 10.16420/j.issn.0513-353x.2020-0119

• 研究论文 • 上一篇    下一篇

芜菁Br14-3-3的克隆及其在非生物胁迫下的表达分析

王若男,李 菊,苗鸿钰,闫海芳*   

  1. 东北林业大学生命科学学院,哈尔滨 150040
  • 出版日期:2020-07-25 发布日期:2020-07-25
  • 基金资助:
    黑龙江省自然科学基金面上项目(C2017001);东北林业大学大学生创新训练项目(201910225335)

Cloning and Expression Under Abiotic Stress of Br14-3-3 in Brassica rapa subsp. rapifera

WANG Ruonan,LI Ju,MIAO Hongyu,and YAN Haifang*   

  1. College of Life Sciences,Northeast Forestry University,Harbin 150040,China
  • Online:2020-07-25 Published:2020-07-25

摘要: 克隆了‘津田芜菁’(Brassica rapa subsp. rapifera‘Tsuda’)通用调节因子14-3-3基因cDNA序列,命名为Br14-3-3(GenBank登录号为MK896872)。该基因全长1 113 bp,开放阅读框全长774 bp,编码含有257个氨基酸的多肽。荧光定量PCR分析Br14-3-3在‘津田芜菁’不同组织中及其在温度、脱水、渗透、ABA和无机盐等非生物胁迫下幼苗中的表达,结果表明该基因在‘津田芜菁’的花中表达量最高,幼苗中次之;低温抑制了Br14-3-3的表达,其他非生物胁迫可诱导该基因表达,暗示Br14-3-3在非生物胁迫应答中发挥功能。

关键词: 津田芜菁, 14-3-3, 基因克隆, 胁迫, 表达

Abstract: In this study,we cloned the general regulatory factor 14-3-3 gene from Brassica rapa subsp. rapifera‘Tsuda’,which was named Br14-3-3 with the accession number of MK896872 in the NCBI GenBank. The full cDNA sequence of the Br14-3-3 gene was 1 113 bp,cotaining an open reading frame of 774 bp encoding a protein of 257 amino acids. We determined the expression of the Br14-3-3 gene in different tissues and under different abiotic stress conditions,such as extreme temperature,dehydration,adverse osmosis,abscisic acid(ABA)and salt stress by quantitative-PCR analysis. The results demonstrated that the highest expression levels of the Br14-3-3 gene could be reached in the flower of Brassica rapa subsp. rapifera‘Tsuda’,followed by the seedling. The expression of the Br14-3-3 gene was upregulated under aforementioned stress conditions except for low temperature,suggesting that the Br14-3-3 gene may play a role in response of abiotic stresses.

Key words: Brassica rapa, 14-3-3, gene cloning, abiotic stress, gene expression

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