http://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
http://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
http://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
http://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2020, Vol. 47 ›› Issue (6): 1153-1164.doi: 10.16420/j.issn.0513-353x.2019-1005

• 研究论文 • 上一篇    下一篇

药用蒲公英低温和高温胁迫下内参基因筛选与相关基因表达分析

乔永刚*,王勇飞,曹亚萍,贺嘉欣,贾孟君,李 政,张鑫瑞,宋 芸   

  1. 山西农业大学生命科学学院,山西太谷 030801
  • 出版日期:2020-06-25 发布日期:2020-06-25
  • 基金资助:
    国家重点研发计划项目(2019YFC1710800)

Reference Genes Selection and Related Genes Expression Analysis under Low and High Temperature Stress in Taraxacum officinale

QIAO Yonggang*,WANG Yongfei,CAO Yaping,HE Jiaxin,JIA Mengjun,LI Zheng,ZHANG Xinrui,and SONG Yun   

  1. College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801,China
  • Online:2020-06-25 Published:2020-06-25

摘要: 为研究不同温度胁迫下药用蒲公英最适内参基因与温度响应相关基因的表达,分别以低温(4 ℃)和高温(38 ℃)胁迫0、3、6、12、24和48 h共12个处理的叶片为材料,选取10个候选内参基因18S、EF1α、TUB、40S、GAPDH、β-actin、ACT11、TUA、60S和SKIP,利用RT-qPCR技术以及geNorm、NormFinder和BestKeeper软件评价其稳定性。利用筛选出的最适内参基因对药用蒲公英12个温度响应基因(AP2/ERF、Dof、ICE1、MYB、bZIP、NTL6、HSF、Gols、HSP、NAC、XCT和WRKY)的表达水平进行定量分析。结果显示:温度胁迫下药用蒲公英最适内参基因为18S和GAPDH;药用蒲公英AP2/ERF、HSF、Glos、HSP、NAC、XCT基因在低温和高温胁迫下均为先上调后下调表达,bZIP、NTL6在温度胁迫下波动变化,Dof、ICE1、MYB在低温胁迫下先上调后下调表达,高温胁迫下持续下调表达,WRKY在高温胁迫下表达量远远高于低温胁迫。依据基因表达量推测,药用蒲公英低温和高温胁迫的响应机制存在差异,AP2/ERF、Dof主要响应低温胁迫,HSF、XCT、WRKY主要响应高温胁迫。

关键词: 药用蒲公英, 温度胁迫, 内参基因, 表达分析

Abstract: In order to research the optimal reference genes and the responsive genes expression of Taraxacum officinale under different temperature stress,the leaves of Taraxacum officinale that were treated for 0,3,6,12,24 and 48 h under low temperature(4 ℃)and high temperature(38 ℃) were used as material. 18S,EF1α,TUB,40S,GAPDH,β-actin,ACT11,TUA,60S and SKIP were selected as candidate reference genes,RT-qPCR technology,geNorm,NormFinder and BestKeeper were taken advantage of evaluating the stability. Utilizing the optimal selected reference genes analyzed the expression level of 12 temperature-responsive genes(AP2/ERF,Dof,ICE1,MYB,bZIP,NTL6,HSF,Gols,HSP,NAC,XCT,WRKY). The results showed that,the optimal reference genes were 18S and GAPDH under different temperature stresses. The expression levels of AP2/ERF,HSF,Glos,HSP,NAC,XCT were firstly up-regulated and then down-regulated during low and high temperature stress. Changes of bZIP and NTL6 were undulant under temperature stresses. Dof,ICE1 and MYB were firstly up-regulated and then down-regulated under low temperature stress,but down-regulated continuously under high temperature. The expression of WRKY under high temperature stress was much higher than that under low temperature stress. Based on the genes expression,it was speculated that the response mechanism of Taraxacum officinale were different under low temperature and high temperature stress. AP2/ERF and Dof mainly responded to low temperature stress,and HSF,XCT and WRKY mainly played a role in high temperature stress.

Key words: Taraxacum officinale, temperature stress, reference gene, expression analysis

中图分类号: