http://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
http://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
http://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
http://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2020, Vol. 47 ›› Issue (3): 409-420.doi: 10.16420/j.issn.0513-353x.2019-0370

• 研究论文 •    下一篇

乙烯参与 ALA-ABA/黑暗调控的苹果叶片气孔运动

胡 淑,安玉艳,汪良驹*   

  1. 南京农业大学园艺学院,南京 210095
  • 出版日期:2020-03-25 发布日期:2020-03-25
  • 基金资助:
    国家自然科学基金项目(31772253,31401820);江苏省高校优势学科项目

Ethylene is Involved in the Regulation of Stomatal Movement by ALA-ABA/dark in Apple Leaves

HU Shu,AN Yuyan,and WANG Liangju*   

  1. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2020-03-25 Published:2020-03-25

摘要: 以‘富士’苹果组培苗离体叶片下表皮为材料,运用不同试剂结合气相色谱、激光共聚焦扫描显微镜、光学显微镜和 qRT-PCR 等技术,从气孔开度、乙烯释放、保卫细胞 H2O2 及黄酮醇水平和基因表达等方面,研究了乙烯在 5–氨基乙酰丙酸(ALA)抑制脱落酸(ABA)或黑暗诱导的气孔关闭过程中的作用。结果表明,ALA 促进苹果叶片乙烯释放,而乙烯生物合成抑制剂氨氧乙基乙烯基甘氨酸(AVG)及乙烯受体抑制剂 1–甲基环丙烯(1-MCP)削弱 ALA 对 ABA 或黑暗诱导气孔关闭的抑制效应。ALA上调乙烯生物合成及信号转导相关基因的表达。AVG 削弱 ALA 抑制 ABA 或黑暗诱导的 H2O2 上升效应,也削弱 ALA 对保卫细胞黄酮醇积累的促进效应。据此认为,外源 ALA 通过上调基因表达,促进叶片保卫细胞中乙烯生物合成和信号转导,诱导黄酮醇积累,清除 ABA 或黑暗诱导增加的 H2O2,抑制气孔关闭,从而为光合作用所需的气体交换打开通道。

关键词: 苹果, ALA, ABA, 黑暗, 乙烯, H2O2, 黄酮醇, 气孔运动

Abstract: In order to investigate the possible regulatory roles of the gaseous hormone ethylene in the inhibitory effect of ALA on ABA- or dark-induced stomatal closure,the stomatal aperture,ethylene production,H2O2 and flavonol levels in guard cells and gene expressions in the abaxial epidermis of detached apple(Malus × domestica Borkh.‘Fuji’)leaves were studied using pharmacology,gas chromatography,laser confocal scanning microscope,optical microscope and qRT-PCR techniques. The results showed that the ALA treatment promoted ethylene release,while both aminoethoxyvinylglycine (AVG),an ethylene biosynthetic inhibitor,and 1-methylcyclopropene(1-MCP),an ethylene receptor inhibitor,repressed the inhibitory effect of ALA on ABA or dark-induced stomatal closure. Exogenous ALA up-regulated expression of the genes related to ethylene biosynthesis and its signal transduction. AVG weakened the inhibition of ALA on ABA- and dark-induced H2O2 accumulation,as well as ALA-induced flavonol accumulation in guard cells. It can be concluded that ALA up-regulates gene expressions to induce ethylene biosynthesis and signal transduction,which positively regulate the flavonol accumulation to reduce the H2O2 content and promote the stomatal opening,benefiting CO2 entrance during photosynthesis.

Key words: apple, ALA, ABA, dark, ethylene, H2O2, flavonol, stomatal movement

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