http://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
http://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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http://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
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http://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
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http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2019, Vol. 46 ›› Issue (8): 1429-1444.doi: 10.16420/j.issn.0513-353x.2018-0961

• 研究论文 •    下一篇

苹果MdCYP707A 家族基因表达分析和MdCYP707A1 的功能鉴定

张婷婷,康 慧,付璐璐,由春香,王小非*,郝玉金*   

  1. 作物生物学国家重点实验室,山东果蔬优质高效生产协同创新中心,山东农业大学园艺科学与工程学院,山东泰安 271018
  • 出版日期:2019-08-25 发布日期:2019-08-25
  • 基金资助:

    国家自然科学基金项目(31430074)

Expression Analysis of Apple MdCYP707A Family Genes and Functional Characterization of MdCYP707A1

ZHANG Tingting,KANG Hui,FU Lulu,YOU Chunxiang,WANG Xiaofei*,and HAO Yujin*   

  1. State Key Laboratory of Crop Biology,Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2019-08-25 Published:2019-08-25

摘要:

对苹果MdCYP707A家族4个成员的蛋白序列进行比对,并进行保守结构域分析发现,MdCYP707A家族成员都含有细胞色素P450单加氧酶结构域。利用荧光定量PCR检测其在苹果不同组织(根、茎、叶、花、果实、种子)中的表达,4个基因在种子中的表达量最高,并且在果实发育不同时期的表达量有明显差异。在苹果种子吸水膨胀和层积过程中的表达分析表明,MdCYP707A家族成员参与了种子萌发过程中ABA的降解。通过检测MdCYP707A基因对不同非生物胁迫(干旱、盐、渗透胁迫)和对ABA的响应,初步认为其在种子萌发中有重要作用,其中,MdCYP707A1对ABA的响应最为明显。另外,利用农杆菌介导的遗传转化的手段,鉴定了MdCYP707A1基因在苹果愈伤组织和拟南芥中的功能,过表达MdCYP707A1能够降低对非生物胁迫的抗性,说明其可能参与ABA的降解过程,同时在拟南芥中异源表达MdCYP707A1能够提高拟南芥种子的萌发率。

关键词: 苹果, MdCYP707A家族基因, ABA, 非生物胁迫, 表达分析

Abstract:

In this study,we analyzed the protein sequences and conserved motif of four apple MdCYP707A family members,and found that the four MdCYP707A family proteins contained a cytochrome P450 monooxygenase domain. The expression levels of the four genes in different tissues (roots,stems,leaves,flowers,fruits,and seeds)were detected by real-time qPCR,and they exhibited the highest level in the seeds. The expression pattern of MdCYP707As also showed significant difference during fruit developmental stages. We also examined the transcription level of the MdCYP707A family members in the process of water swelling and stratification of apple seeds,and found that the MdCYP707A family members were involved in the degradation of ABA during seed germination. Furthermore,the response of MdCYP707A genes to abiotic stresses(such as drought,salt,osmotic stress)and ABA treatments indicated their important roles in seed germination,among them,the response to ABA is most obvious. In addition,the function of MdCYP707A1 gene was identified through Agrobacterium-mediated genetic transformation to apple calli and Arabidopsis. The results demonstrated that overexpression of MdCYP707A1 reduced the resistance to abiotic stress,which suggests that it may be involved in ABA degradation,and ectopic expression of MdCYP707A1 in Arabidopsis increased the germination rate.

Key words: apple, MdCYP707A family gene, ABA, abiotic stress, expression analysis

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