园艺学报 ›› 2019, Vol. 46 ›› Issue (8): 1543-1552.doi: 10.16420/j.issn.0513-353x.2018-0891

• 研究论文 • 上一篇    下一篇


张慧琳,朱 婉,田 丽,张 蔚*   

  1. 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉 430070
  • 出版日期:2019-08-25 发布日期:2019-08-25
  • 基金资助:


Characterization and Expression Analysis of Petunia PhZPT2-12 Transcription Factor Related to Cold Response

ZHANG Huilin,ZHU Wan,TIAN Li,and ZHANG Wei*   

  1. College of Horticulture & Forestry Sciences,Huazhong Agricultural University,Key Laboratory of Horticultural Plant Biology(Ministry of Education),Wuhan 430070,China
  • Online:2019-08-25 Published:2019-08-25


从矮牵牛‘H’自交系中分离获得1个响应低温胁迫的C2H2型锌指蛋白基因PhZPT2-12,其开放阅读框为525 bp,编码1条含有两个典型的C2H2型保守结构域,长度为174 aa的氨基酸多肽链。系统进化树分析表明,PhZPT2-12与已报道的SlZF3亲缘关系较近。亚细胞定位和转录激活活性分析显示,PhZPT2-12定位于细胞核,是一种核蛋白,并且其全长在酵母细胞中不具有转录激活活性。半定量RT-PCR表达分析结果表明,正常生长条件下PhZPT2-12在矮牵牛根、茎、叶中表达量较高,而在成熟的花中仅有微弱表达;在低温、高盐和干旱胁迫处理下PhZPT2-12表现出不同程度的上调,其中对低温胁迫最为敏感,上调倍数最高,初步推测该基因与矮牵牛应答低温、高盐等非生物逆境相关。

关键词: 矮牵牛, 低温胁迫, C2H2锌指蛋白, 转录因子, 表达分析


Gene expression microarray of petunia was applied to select cold responsive genes,from which a C2H2-type zinc-finger protein gene PhZPT2-12 was discovered. Using inbred line‘H’as the template,PhZPT2-12 was isolated. It contains an Open Reading Frame(ORF)of 525 bp,encoding a peptide of 174 aa with two classical C2H2 domains. Phylogenetic tree analysis demonstrated that PhZPT2-12 is closely related to SlZF3 reported. Subcellular localization and transactivation activity assays showed that PhZPT2-12 was targeted to nucleus and owned no transcriptional activation activity. Semi-quantitative RT-PCR analysis revealed that the expression of PhZPT2-12 was higher in roots,stems and leaves,whereas much lower in mature flowers under normal growth conditions. Stress induced expression analysis showed that the expression of PhZPT2-12 could be induced by cold rapidly,also by salt and drought in different degrees. It is suggested that PhZPT2-12 is associated with the responses to cold,salt and other abiotic stresses.

Key words: petunia, cold stress, C2H2 zinc-finger protein, transcription factor, expression analysis