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园艺学报 ›› 2019, Vol. 46 ›› Issue (12): 2299-2308.doi: 10.16420/j.issn.0513-353x.2018-0676

• 研究论文 • 上一篇    下一篇

1-MCP对‘玉露香’梨采后果实品质和叶绿素保持的影响

马风丽,杜艳民,王 阳,佟 伟,刘佰霖,王文辉*,贾晓辉*   

  1. 中国农业科学院果树研究所,辽宁兴城 125100
  • 出版日期:2019-12-25 发布日期:2019-12-25
  • 基金资助:
    国家重点研发计划项目(2016YFD0400903-06);国家现代农业产业技术体系建设专项资金项目(CARS-29-19);中国农业科学院科技创新工程项目(CAAS-ASTIP)

Effect of 1-Methylcyclopropene(1-MCP)on Quality and Chlorophyll Maintenance of Postharvest‘Yuluxiang’Pear

MA Fengli,DU Yanmin,WANG Yang,TONG Wei,LIU Bailin,WANG Wenhui*,and JIA Xiaohui*   

  1. Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100,Liaoning,China
  • Online:2019-12-25 Published:2019-12-25

摘要: 探讨1–甲基环丙烯(1-MCP)对‘玉露香’梨采后果实品质和叶绿素保持的影响,为其采后保鲜技术的提出提供理论基础。以‘玉露香’梨为试材,用1.0 μL ? L-1 1-MCP熏蒸处理12 h,置于常温(20 ℃)下,测定梨果实颜色、叶绿素含量等与果皮颜色等相关的生理指标,果实硬度、可溶性固形物、可滴定酸、维生素C含量等品质指标,以及呼吸速率、乙烯释放量等,并通过实时荧光定量PCR技术检测与叶绿素降解相关的基因表达水平。结果表明:1-MCP对常温贮藏条件下‘玉露香’梨果实的硬度和可溶性固形物含量无显著影响,但显著抑制了可滴定酸和维生素C含量的下降、PbETR1和PbETR2的表达以及呼吸速率,推迟乙烯释放高峰出现的时间;1-MCP处理可以抑制PbCLH1的表达,推迟叶绿素分解代谢途径中的下游反应,从而延缓果皮叶绿素的降解,对‘玉露香’梨果实采后品质和叶绿素具有较好的维持作用。

关键词: 梨, 1-MCP, 品质, 叶绿素降解, 基因表达

Abstract: Effect of 1-methylcyclopropene(1-MCP)on chlorophyll degradation and quality maintenance of postharvest of‘Yuluxiang’pear fruits were investigated,providing a theoretical basis for the‘Yuluxiang’pear about preservation technology. In this study,two groups of ‘Yuluxiang’pear were used as experimental materials,one group was treated with 1.0 μL ? L-1 1-MCP at 20 ℃,while the other group was used as control and kept at 20 ℃ without 1-MCP treatment. Chlorophyll content and fruit firmness,soluble solids,titratable acid,vitamin C content,respiration rate and ethylene production were measured. Moreover,the expression level of related genes to chlorophyll degradation were detected by real-time quantitative PCR. The results showed that the firmness and soluble solids content of ‘Yuluxiang’pear were unsignificantly affected between 1-MCP treatment and the control. But 1-MCP could significantly inhibited the decrease of titratable acid content and vitamin C content,the expression of PbETR1 and PbETR2,and delayed the respiration rate and ethylene retention time;1-MCP treatment could inhibit the expression of PbCLH1 gene,delay the downstream reaction in the pathway of chlorophyll catabolism,so as to delay the degradation of chlorophyll,and maintain the quality and chlorophyll of‘Yuluxiang’pear fruit during storage.

Key words: pear, 1-MCP, quality, chlorophyll degradation, gene expression

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