Abstract:

Through RT-PCR technique 1 324 bp cDNA sequence of S locus glycoprotein gene (BcSLG) was obtained from self-incompatible line with specific primers in Brassica campestris ssp.chinensis. Sequence analysis indicated BcSLG contained one open reading frame, which encoded 432 amino acids with 12 conserved cysteine residues and 7 potential N-linked glycosylation sites. Sequence alignment and phyologenetic analysis revealed BcSLG had high similarity to SLG from other plants and had closest phylogenetic relationship to that in Brassica campestris L. and Brassica oleracea L. Real-time PCR analysis showed BcSLG was highly expressed in stigmas, much lower in buds and lowest in leaves of self-incompatible line. However, in self-compatible line BcSLG was expressed in an even lower level whether in stigmas, buds or leaves.

Key words: Brassica campestris L. ssp. chinensis, S locus glycoprotein gene, real-time PCR, quantitative analysis of gene expression