Expression analysis of NTMADS1 gene in Narcissus tazetta var. chinensis and study on its transformation in Arabidopsis thaliana

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (2): 245-250.

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Expression analysis of NTMADS1 gene in Narcissus tazetta var. chinensis and study on its transformation in Arabidopsis thaliana

CHEN Duan-fen^1,3,GAO Zhi-min^1*,and PENG Zhen-hua^2**

(¹International Center for Bamboo and Rattan, SFA key Laboratory of Bamboo and Rattan Science and Technology, Beijing 100102, China；²Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China；³College of Horticulture , Agriculture University of Hebei , Baoding 071001, China)

Received:2008-09-09 Revised:2009-01-23 Online:2009-02-25 Published:2009-02-25
Contact: GAO Zhi-min;PENG Zhen-hua

Abstract

Abstract:

The expression pattern of NTMADS1 gene, a flower organ identity gene from Narcissus tazetta var. chinensis, was analyzed by RT-PCR. The results revealed that the transcript of NTMADS1 was not detectable in vegetative tissues, but only in flowers. Within open flower organs, transcript levels were much higher in stamen and corana than in pistil and barely detectable in petals. NTMADS1 gene was subcloned into the multiple cloning sites of pBI121 vector drived by 35S promoter, and was transferred into Arabidopsis thaliana by Agrobacterium tumefaciens. Ectopic expression of NTMADS1 in transgenic A. thaliana plants resulted curly leaves, early flowering and shape-changed flowers.

Key words: Narcissus tazetta var. chinensis, NTMADS1 gene, tissue specific expression, transformation, Arabidopsis thaliana

CLC Number:

S 682.2⁺ 1

CHEN Duan-fen;GAO Zhi-min;and PENG Zhen-hua . Expression analysis of NTMADS1 gene in Narcissus tazetta var. chinensis and study on its transformation in Arabidopsis thaliana [J]. ACTA HORTICULTURAE SINICA, 2009, 36(2): 245-250.

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Expression analysis of NTMADS1 gene in Narcissus tazetta var. chinensis and study on its transformation in Arabidopsis thaliana

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