Abstract:

Two CBF/DREB1 genes named CcCBFc and CcCBFd were isolated from Cinnamomum camphora with the help of homologous cloning strategy and RACE（rapid amplification of cDNA ends）technique，and their GenBank accession numbers are KP336741 and KP336742，respectively. The full-length cDNA sequences of the two CcCBF genes were 897 and 1 010 bp，including an open reading frame（ORF）of 654 and 621 bp，respectively，and no introns existed in their coding regions. The CcCBFc and CcCBFd genes encode 217 and 206 amino acids with predicted molecular masses of 24.0 and 22.9 kD，and pI of 5.01 and 5.11. CcCBFc and CcCBFd were classified into the CBF/DREB1 subfamily，and had relatively close evolutionary relationship with dicotyledons，according to the homology comparison and phylogeny tree analysis of the deduce amino acids sequences with other CBF/DREB homologues. The expression level of both CcCBFc and CcCBFd could be upregulated by all tested treatments such as cold （4 ℃），drought（20% PEG），salinity（250 mmol ? L-1 NaCl）and ABA（100 μmol ? L-1）. The observations presented in this study might indicate that both CcCBFc and CcCBFd were involved in response to various abiotic stresses.

Key words: Cinnamomum camphora, CBF/DREB1, gene cloning, expression analysis