Abstract: Anthocyanin accumulation in plants is influenced by a number of positive and negative regulators. Previous studies mainly focused on the transcriptional activators，and several transcriptional repressors have been shown to negatively regulate anthocyanin biosynthesis recently. It is becoming increasingly clear that negative regulators play important roles in precise regulation of anthocyanin biosynthesis responding to different developmental and environmental signals. In this paper，the latest advances in research progress of negative regulators in anthocyanin accumulation were reviewed from the aspects of structure，mechanism and regulatory pathway. These repressors can be divided into three types depending on their regulatory mechanism：Transcriptional repressors，posttranscriptional repressors and those with unclear mechanism. Finally，the new research directions of these regulators were discussed.

Key words: anthocyanin, accumulation, negative regulator