Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (6): 1067-1078.doi: 10.16420/j.issn.0513-353x.2020-0674

• Research Papers • Previous Articles     Next Articles

Construction of Yeast Two Hybrid Library and Screening of FvM4K1 Interacting Protein in the Fruit of Fragraria vesca

GU Si, LIU Lu, LI Anran, ZHANG Weiwei, ZHAO Shuaiqi, XING Yu()   

  1. Advanced Innovation Center for Forest Molecular Design and Breeding,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China
  • Received:2020-10-19 Revised:2021-03-03 Online:2021-06-25 Published:2021-07-07
  • Contact: XING Yu


cDNA library of strawberry fruit at different stages was constructed and the proteins interacting with strawberry mitogen activated kinase FvM4K1 were screened which revealed the molecular mechanism of FvM4K1 in regulating strawberry fruit development. The total RNA ofFragraria vesca ‘Hawaii 4'was extracted at different fruit development stages related to FvM4K1,and the strawberry cDNA library was established. Specifically,the library capacity was 9.6 × 10 6 CFU,the recombination rate was 96%,and the average length of inserted fragment was more than 1 000 bp. At the same time,the bait vector pGBKT7-FvM4K1 was constructed,and no self-activation activity was detected in yeast. Then 13 proteins interacting with FvM4K1 were screened from the library by co-transformation. The function of these candidate proteins mainly focused on floral development such as pollen development and pollen tube elongation,also affecting flowering time and so on. Meanwhile,the Gene Ontology(GO)pathway annotation of these proteins showed that they mainly participated in cell metabolism process and molecular processes with catalytic activity. Besides,the 13 proteins screened had different expression patterns during fruit development,and four of them were predicted to be closely related to strawberry fruit development.

Key words: strawberry, Fragaria ananassa, cDNA library, MAPK

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