Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 531-538.doi: 10.16420/j.issn.0513-353x.2020-0230

• Research Papers • Previous Articles     Next Articles

The Effect of Stu-miR156 Silencing by STTM Technology on Potato Lateral Root Development

LUO Hongyu1, YANG Jiangwei1, FENG Ya1, ZHANG Huanhuan1, LIU Shengyan1, ZHANG Ning1,2,*(), SI Huaijun1,2,*   

  1. 1College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
    2Gansu Provincial Key Laboratory of Aridland Crop Science,Lanzhou 730070,China
  • Received:2020-05-10 Online:2021-03-25 Published:2021-04-02
  • Contact: ZHANG Ning,SI Huaijun


To elucidate the biological function of Stu-miR156 in potato lateral root development,plantlets in vitro of potato cultivar‘Desiree’were used as dornors. Using STTM(Short tandem target mimic,STTM)technology,the Stu-miR156 silencing expression vector was successfully constructed and further the transgenic plants were obtained viaAgrobacterium-mediated method. qRT-PCR was used to determine the differential expression of Stu-miR156 and its target gene StSPL9in the transgenic potato plants. We constructed a pCAM-GFP-StSPL9 fusion protein expression vector and transformed it into tobacco,thereby confirming that StSPL9 was localized in the cytoplasm and nucleus. The result of qRT-PCR showed that the expression levels of Stu-miR156 in roots,stems and leaves were severely inhibited,while the target gene StSPL9 was up-regulated in the transgenic potato lines L1 and L2. The number of lateral roots of the transgenic plants was significantly reduced and the growth is inhibited compared with the wild type plants.

Key words: potato, Stu-miR156, STTM, StSPL9, genetic transformation, lateral root

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