Abstract: An efficient regeneration protocol was developed through shoot regeneration from leaf exp lants of A ltem anthera f icoidea ‘Ruliginosa’. The results showed that light green calli could be formed from all the leaf explants of cultured in vitro for 20 days onMS medium containing 6-BA 1.0 mg/L, 4-PU 〔N-phenyl-N’-(2-chloro-4-pyridyl) urea 〕1.0 mg/L and NAA 0.1mg/L. Adventitious shoots in purp le could be developed from the calli at the frequency of 45.31% on MS medium supplemented with 1.0 mg/L 6-BA and 0.4 mg/L NAA. About 6% of the regenerated variants buds with green leaf-colour and tiny leaves were also observed to form on the same medium. Adventitious purple shoots could be rooted and formed regenerated plants on 1 /2MS medium supplemented with 0.4 mg/L NAA. The survival rates of regenerated plants were more than 85%.

Key words: Altemanthera ficoidea, Leaf, Tissue culture, Plantlet regeneration