Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 600-608.doi: 10.16420/j.issn.0513-353x.2020-0446

• New Technology and New Mathod • Previous Articles     Next Articles

Development and Application of an Efficient Method for the Amylose/ Amylopectin Ratio Determination in Potato Tubers

SONG Yuhao, SHI Weiling, ZHANG Jiao, DING Zhenyu, PENG Hongxian, JIANG Chunyan, MA Qiuqin, LI Zhijing, ZHAO Yong, TANG Daobin, ZHANG Kai, WANG Jichun, LIU Xun()   

  1. Chongqing Key Laboratory of Biology and Genetic Improvement in Tuber and Root Crops,College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China
  • Received:2020-07-10 Online:2021-03-25 Published:2021-04-02
  • Contact: LIU Xun


Potato starch composition(amylose/amylopectin,AM/AP)influences functional characteristics for food and other industrial applications. The determination of AM/AP ratio is of great significance for rational utilization of starch and breeding special varieties with specific starch components. The dual-wavelength method for determination of AM and AP contents was established in a 96-well plate format firstly. It was identified that the AM contents was dramatically overestimated when the AM/AP ratio of tested samples exceeded the AM/AP ratio(33.3%)of the standard curve. Furthermore,the new standard curve of potato AM/AP ratio and wavelength under the maximum absorption peak were established:y= 601.88x0.0215,R2= 0.9999. This method further improves the accuracy and is applicable over large ranges of AM/AP ratio needed for screening. This method was used to determine the AM/AP ratio from 198 potato varieties(lines)and a few transgenic tubers with high amylose contents. The result revealed that the range of AM contents in our potato genetic resources is 17.4%-33.3%. In conclusion,a simple and efficient method for the accurate estimation of the potato starch composition was established which is especially suitable for the detection of genetic breeding materials and improvement starch composition lines.

Key words: potato, starch component, detection system, amylose, amylopectin

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