Advances in Plant Promoter Research

doi:10.16420/j.issn.0513-353x.2024-0408

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 1065-1092.doi: 10.16420/j.issn.0513-353x.2024-0408

• Reviews • Previous Articles Next Articles

Advances in Plant Promoter Research

ZHANG Zhiyuan, WANG Dan, XI Zhumei, WANG Xianhang^*()

College of Wine，Northwest Agriculture and Forestry University，Yangling，Shaanxi 712100，China

Received:2024-07-02 Revised:2025-01-13 Online:2025-05-08 Published:2025-04-25
Contact: WANG Xianhang

Abstract

Abstract:

In this paper， the structural characteristics of different types of promoters and their roles in gene function research，metabolic engineering，and gene editing（CRISPR/Cas9）were discusses，and summarized the content and methods for promoter identification，aiming to provide insights for a deeper understanding and investigation of the structural characteristics and regulatory mechanisms of plant promoters.

Key words: promoter, genetic engineering, metabolic engineering, CRISPR/Cas9

ZHANG Zhiyuan, WANG Dan, XI Zhumei, WANG Xianhang. Advances in Plant Promoter Research[J]. Acta Horticulturae Sinica, 2025, 52(4): 1065-1092.

Figures/Tables 7

References 164

[1]	Ahammed G J, Li C X, Li X, Liu A, Chen S, Zhou J. 2021. Overexpression of tomato RING E3 ubiquitin ligase gene SlRING1 confers cadmium tolerance by attenuating cadmium accumulation and oxidative stress. Physiologia plantarum, 173 (1):449-459.
[2]	Amack S C, Antunes M S. 2020. CaMV35S promoter-A plant biology and biotechnology workhorse in the era of synthetic biology. Current Plant Biology,24:100179.
[3]	Archer L, Mondal H A, Behera S, Twayana M, Patel M, Louis J, Nalam V J, Keereetaweep J, Chowdhury Z, Shah J. 2023. Interplay between MYZUS PERSICAE-INDUCED LIPASE 1 and OPDA signaling in limiting green peach aphid infestation on Arabidopsis thaliana. Journal of Experimental Botany, 74 (21):6860-6873.
[4]	Atkinson R G, Bolitho K M, Wright M A, Iturriagagoitia-Bueno T, Reid S J, Ross G S. 1998. Apple ACC-oxidase and polygalacturonase:ripening-specific gene expression and promoter analysis in transgenic tomato. Plant Molecular Biology,38:449-460.
[5]	Bai J, Wang X, Wu H, Ling F, Zhao Y, Lin Y, Wang R. 2020. Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters. Plant Biotechnology Journal, 18 (3):668-678.
[6]	Bhullar S, Datta S, Advani S, Chakravarthy S, Gautam T, Pental D, Burma P K. 2007. Functional analysis of cauliflower mosaic virus 35S promoter:re-evaluation of the role of subdomains B5,B4 and B2 in promoter activity. Plant Biotechnology Journal, 5 (6):696-708.
[7]	Boruah R R, Konwar T, Nath P K, Acharjee S, Sarmah B K. 2023. Activity of Arabidopsis Rubisco small subunit promoter in various tissues of chickpea. 3 Biotech, 13 (3):89.
[8]	Brown R L, Kazan K, McGrath K C, Maclean D J, Manners J M. 2003. A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis. Plant Physiology, 132 (2):1020-1032.
[9]	Butler J E, Kadonaga J T. 2002. The RNA polymerase II core promoter:a key component in the regulation of gene expression. Genes & Development, 16 (20):2583-2592.
[10]	Büttner M, Singh K B. 1997. Arabidopsis thaliana ethylene-responsive element binding protein(AtEBP),an ethylene-inducible,GCC box DNA-binding protein interacts with an ocs element binding protein. Proceedings of the National Academy of Sciences, 94 (11):5961-5966.
[11]	Cai C, Wang W, Ye S, Zhang Z, Ding W, Xiang M, Wu C, Zhu Q. 2020. Overexpression of a novel Arabidopsis gene SUPA leads to various morphological and abiotic stress tolerance alternations in Arabidopsis and Poplar. Frontiers in Plant Science,11:560985.
[12]	Cai M, Wei J, Li X, Xu C, Wang S. 2007. A rice promoter containing both novel positive and negative cis-elements for regulation of green tissue-specific gene expression in transgenic plants. Plant Biotechnology Journal, 5 (5):664-674.
[13]	Chen J, Ren C, Wang J, Xian B, Wang R, Wu Q, Pei J. 2023a. pCtFSG2,a flower-specific promoter with suitable promoter activity in safflower. Journal of Plant Biochemistry and Biotechnology, 32 (3):478-486.
[14]	Chen K, Liu W, Li X, Li H. 2021a. Overexpression of GmGASA32 promoted soybean height by interacting with GmCDC25. Plant Signaling & Behavior, 16 (2):1855017.
[15]	Chen L, Jiang B, Wu C, Sun S, Hou W, Han T. 2015. The characterization of GmTIP,a root-specific gene from soybean,and the expression analysis of its promoter. Plant Cell,Tissue and Organ Culture,121:259-274.
[16]	Chen L, Li Y, Wang Y, Li W, Feng X, Zhao L. 2021b. Use of high resolution spatiotemporal gene expression data to uncover novel tissue-specific promoters in tomato. Agriculture, 11 (12):1195.
[17]	Chen L, Su P, Yang G, H G, Gao C. 2023b. PSG076promoter shows late pollen-specific activity in wheat(Triticum aestivum L.). Plant Molecular Biology Reporter, 41 (4):690-698.
[18]	Chen Z, Zhou L, Jiang P, Lu R, Halford N G, Liu C. 2021c. Genome-wide identification of sucrose nonfermenting-1-related protein kinase(SnRK) genes in barley and RNA-seq analyses of their expression in response to abscisic acid treatment. BMC Genomics, 22 (1):300.
[19]	Choi H I, Hong J H, Ha J O, Kang J Y, Kim S Y. 2000. ABFs,a family of ABA-responsive element binding factors. Journal of Biological Chemistry, 275 (3):1723-1730.
[20]	Chow C N, Lee T Y, Hung Y C, Li G Z, Tseng K C, Liu Y H, Kuo P L, Zheng H Q, Chang W C. 2019. PlantPAN3.0:a new and updated resource for reconstructing transcriptional regulatory networks from ChIP-seq experiments in plants. Nucleic Acids Research, 47 (D1):D1155-D1163.
[21]	Christensen A H, Sharrock R A, Quail P H. 1992. Maize polyubiquitin genes:structure,thermal perturbation of expression and transcript splicing,and promoter activity following transfer to protoplasts by electroporation. Plant Molecular Biology,18:675-689.
[22]	Chung M Y, Naing A H, Vrebalov J, Shanmugam A, Lee D J, Park I H, Kim C K, Giovannon J. 2020. The use of SlAdh2 promoter as a novel fruit-specific promoter in transgenic tomato. Journal of Plant Biotechnology, 47 (2):172-178.
[23]	Cobb R E, Sun N, Zhao H. 2013. Directed evolution as a powerful synthetic biology tool. Methods, 60 (1):81-90.
[24]	Colebrook E H, Thomas S G, Phillips A L, Hedden P. 2014. The role of gibberellin signalling in plant responses to abiotic stress. Journal of Experimental Biology, 217 (1):67-75.
[25]	Dasgupta K, Hotton S, Belknap W, Syed Y, Dardick C, Thilmony R, Thomson J G. 2020. Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology. BMC Biotechnology,20:1-15.
[26]	de Ruijter N C A, Verhees J, Van Leeuwen W, Van Der Krol A R. 2003. Evaluation and comparison of the GUS,LUC and GFP reporter system for gene expression studies in plants. Plant Biology, 5 (2):103-115.
[27]	de Silva W S I, Perera M M N, Perera K L N S, Wickramasuriya A M, Jayasekera G A U. 2017. In silico analysis of osr40c1 promoter sequence isolated from Indica variety Pokkali. Rice science, 24 (4):228-234.
[28]	Deb D, Dey N. 2019. Synthetic salicylic acid inducible recombinant promoter for translational research. Journal of Biotechnology,297:9-18.
[29]	Dong G, Fan M, Wang H, Leng Y, Sun J, Huang J, Zhang H, Yan J. 2023. Functional characterization of TkSRPP promoter in response to hormones and wounding stress in transgenic tobacco. Plants, 12 (2):252.
[30]	Dong H, Liu L, Fan X, Asghar S, Li Y, Wang Y, Xu X, Wu T, Zhang X, Qiu C, Han Z, Li W. 2019. The artificial promoter rMdAG2I confers flower-specific activity in Malus. International Journal of Molecular Sciences, 20 (18):4551.
[31]	Elliott A R, Campbell J A, Dugdale B, Brettell R I S, Grof C P L. 1999. Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells. Plant Cell Reports,18:707-714.
[32]	Fan J, Jiang F, Sun H, He T, Liu Y, Jiao G, Ahmad B, Bokhari S A M, Chen Q, Wen Z. 2023. Expression analysis of trihelix transcription factor family in strawberries and functional characterization of FvTrihelix6. Horticulturae, 9 (6):633.
[33]	Fang R X, Nagy F, Sivasubramaniam S, Chua N H. 1989. Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants. The Plant Cell, 1 (1):141-150.
[34]	Feng C, Su H, Bai H, Wang R, Liu Y, Guo X, Liu C, Zhang J, Yuan J, Birchler J A, Han F. 2018. High-efficiency genome editing using a dmc1 promoter-controlled CRISPR/Cas 9 system in maize. Plant Biotechnology Journal, 16 (11):1848-1857.
[35]	Feng Z, Liu N, Zhang G, Bu Y, Wang B, Gong Y. 2022. Promoter of vegetable soybean GmTIP1;6 responds to diverse abiotic stresses and hormone signals in transgenic Arabidopsis. International Journal of Molecular Sciences, 23 (20):12684.
[36]	Fernandez-Moreno J P, Yaschenko A E, Neubauer M, Marchi A J, Zhao C, Ascencio-Ibanez J T, Alonso J M, Stepanova A N. 2024. A rapid and scalable approach to build synthetic repetitive hormone-responsive promoters. Plant Biotechnology Journal, 22 (7):1942-1956
[37]	Field A, Adelman K. 2020. Evaluating enhancer function and transcription. Annual Review of Biochemistry,89:213-234.
[38]	Fitzgerald H A, Chern M S, Navarre R, Ronald P C. 2004. Overexpression of (At)NPR1 in rice leads to a BTH-and environment-induced lesion-mimic/cell death phenotype. Molecular Plant-Microbe Interactions, 17 (2):140-151.
[39]	Freitas E O, Melo B P, Lourenço-Tessutti I T, Arraes F B, Amorim R M, Lisei-de-Sá M E, Costa J A, Leite A G B, Faheem M, Ferreira M A, Morgante C V, Fontes E P B, Grossi-de-Sa M F. 2019. Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses:potential tool for biotechnological application. BMC Biotechnology,19:1-14.
[40]	Gatz C, Lenk I. 1998. Promoters that respond to chemical inducers. Trends in Plant Science, 3 (9):352-358.
[41]	Geem K R, Kim D H, Lee D W, Kwon Y, Lee J, Kim J H, Hwang I. 2019. Jasmonic acid-inducible TSA 1 facilitates ER body formation. The Plant Journal, 97 (2):267-280.
[42]	Gong P, Wei R, Li Y, Wang R, Tang Y, Wang L, Zhu H, Wang Y, Zhang C. 2019. Molecular cloning and functional characterization of a seed-specific VvβVPE gene promoter from Vitis vinifera. Planta,250:657-665.
[43]	Gong Q, Wang Y, Jin Z, Hong Y, Liu Y. 2022. Transcriptional and post-transcriptional regulation of RNAi-related gene expression during plant-virus interactions. Stress Biology, 2 (1):33.
[44]	He Y, Zhang T, Sun H, Zhan H, Zhao Y. 2020. A reporter for noninvasively monitoring gene expression and plant transformation. Horticulture Research, 7 (1):152.
[45]	He Z M, Jiang X L, Qi Y, Luo D Q. 2008. Assessment of the utility of the tomato fruit-specific E8 promoter for driving vaccine antigen expression. Genetica,133:207-214.
[46]	Hua L, Li M, Sun X, Wang J, Li Z, Xu Y, Hu S, Chen H. 2012. A dual color fluorescent reporter system for the real time detection of promoter activity. Biotechnology Letters,34:823-830.
[47]	Huang L Y, Zhang F, Qiao Q I N, Wang W S, Zhang T, Fu B Y. 2015. Identification and validation of root-specific promoters in rice. Journal of Integrative Agriculture, 14 (1):1-10.
[48]	Jakočiūnas T, Pedersen L E, Lis A V, Jensen M K, Keasling J D. 2018. CasPER,a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9. Metabolic Engineering,48:288-296.
[49]	Jameel A, Noman M, Liu W, Ahmad N, Wang F, Li X, Li H. 2020. Tinkering cis motifs jigsaw puzzle led to root-specific drought-inducible novel synthetic promoters. International Journal of Molecular Sciences, 21 (4):1357.
[50]	Jefferson R A, Kavanagh T A, Bevan M W. 1987. GUS fusions:beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal, 6 (13):3901-3907.
[51]	Jiang Y, Yu D. 2015. WRKY transcription factors:links between phytohormones and plant processes. Science China. Life Sciences, 58 (5):501.
[52]	Jin B, Sheng Z, Muhammad I, Chen J, Yang H. 2019. Cloning and functional analysis of the promoter of a stress-inducible gene(Zmap)in maize. PLoS ONE, 14 (2):e0211941.
[53]	Jores T, Tonnies J, Wrightsman T, Buckler E S, Cuperus J T, Fields S, Queitsch C. 2021. Synthetic promoter designs enabled by a comprehensive analysis of plant core promoters. Nature Plants, 7 (6):842-855.
[54]	Jupin I, Chua N H. 1996. Activation of the CaMV as-1 cis-element by salicylic acid:differential DNA-binding of a factor related to TGA1a. The EMBO Journal, 15 (20):5679-5689.
[55]	Kagaya Y, Hobo T, Murata M, Ban A, Hattori T. 2002. Abscisic acid-induced transcription is mediated by phosphorylation of an abscisic acid response element binding factor,TRAB1. The Plant Cell, 14 (12):3177-3189.
[56]	Kesanakurti D, Kolattukudy P E, Kirti P B. 2012. Fruit-specific overexpression of wound-induced tap1 under E8 promoter in tomato confers resistance to fungal pathogens at ripening stage. Physiologia Plantarum, 146 (2):136-148.
[57]	Khadanga B, Chanwala J, Sandeep I S, Dey N. 2021. Synthetic promoters from strawberry vein banding virus(SVBV)and dahlia mosaic virus (DaMV). Molecular Biotechnology, 63 (9):792-806.
[58]	Kim A Y, Kim H M, Ma S H, Park S Y, Dat M T, Jang G, Joung Y H. 2019. The promoter of tomato HISTIDINE DECARBOXYLASE A is fruit-specific,and its expression is stably maintained in fruits during ripening. Plant Biotechnology Reports,13:43-50.
[59]	Kim H M, Park S H, Park S Y, Ma S H, Do J H, Kim A Y, Jeon M J, Shim J S, Joung Y H. 2022. Identification of essential element determining fruit-specific transcriptional activity in the tomato HISTIDINE DECARBOXYLASE A gene promoter. Plant Cell Reports, 41 (8):1721-1731.
[60]	Kneissl M L, Deikman J. 1996. The tomato E8 gene influences ethylene biosynthesis in fruit but not in flowers. Plant Physiology, 112 (2):537-547.
[61]	Kobayashi S, Goto-Yamamoto N, Hirochika H. 2004. Retrotransposon-induced mutations in grape skin color. Science, 304 (5673):982.
[62]	Konishi M, Donner T J, Scarpella E, Yanagisawa S. 2015. MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells. Journal of Experimental Botany, 66 (1):283-291.
[63]	Koo J, Kim Y, Kim J, Yeom M, Lee I C, Nam H G. 2007. A GUS/luciferase fusion reporter for plant gene trapping and for assay of promoter activity with luciferin-dependent control of the reporter protein stability. Plant and Cell Physiology, 48 (8):1121-1131.
[64]	Köster J, Thurow C, Kruse K, Meier A, Iven T, Feussner I, Gatz C. 2012. Xenobiotic-and jasmonic acid-inducible signal transduction pathways have become interdependent at the Arabidopsis CYP81D11 promoter. Plant Physiology, 159 (1):391-402.
[65]	Krogan N T, Marcos D, Weiner A I, Berleth T. 2016. The auxin response factor MONOPTEROS controls meristem function and organogenesis in both the shoot and root through the direct regulation of PIN genes. New Phytologist, 212 (1):42-50.
[66]	Kummari D, Palakolanu S R, Kishor P K, Bhatnagar-Mathur P, Singam P, Vadez V, Sharma K K. 2020. An update and perspectives on the use of promoters in plant genetic engineering. Journal of Biosciences,45:1-24.
[67]	Lee D K, Jung H, Jang G, Jeong J S, Kim Y S, Ha S H, Choi Y D, Kim J K. 2016. Overexpression of the OsERF71 transcription factor alters rice root structure and drought resistance. Plant Physiology, 172 (1):575-588.
[68]	Lee M, Dominguez-Ferreras A, Kaliyadasa E, Huang W J, Antony E, Stevenson T, Lehmann S, Schäfer P, Knight M R, Ntoukakis V, Knight H. 2021. Mediator subunits MED16,MED14,and MED2 are required for activation of ABRE-dependent transcription in Arabidopsis. Frontiers in Plant Science,12:649720.
[69]	Lescot M, Déhais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouzé P, Rombauts S. 2002. PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 30 (1):325-327.
[70]	Li C, Li W, Zhou Z, Chen H, Xie C, Lin Y. 2020a. A new rice breeding method:CRISPR/Cas 9 system editing of the Xa13 promoter to cultivate transgene-free bacterial blight-resistant rice. Plant Biotechnology Journal, 18 (2):313.
[71]	Li R, Zhu F, Duan D. 2020b. Function analysis and stress-mediated cis-element identification in the promoter region of VqMYB15. Plant Signaling & Behavior, 15 (7):1773664.
[72]	Li X, Niu G, Fan Y, Liu W, Wu Q, Yu C, Wang J, Xiao Y, Hou L, Jin D, Chen S, Hu R, Yang Y, Pei Y. 2023. Synthetic dual hormone-responsive promoters enable engineering of plants with broad-spectrum resistance. Plant Communications, 4 (4):100596.
[73]	Li Y, Yu L, Wang Q, Zhao X, Li X, Qi B. 2021. Analysis of the promoter of Emb5 from Zea mays identifies a region of 523 bp responsible for its embryo-specific activity. Plant Molecular Biology Reporter,39:288-300.
[74]	Liao D, Chen X, Chen A, Wang H, Liu J, Liu J, Gu M, Sun S, Xu G. 2015. The characterization of six auxin-induced tomato GH3 genes uncovers a member,SlGH3.4,strongly responsive to arbuscular mycorrhizal symbiosis. Plant and Cell Physiology, 56 (4):674-687.
[75]	Lim C J, Lee H Y, Kim W B, Lee B S, Kim J, Ahmad R, Kim H A, Yi S Y, Hur C G, Kwon S Y. 2012. Screening of tissue-specific genes and promoters in tomato by comparing genome wide expression profiles of Arabidopsis orthologues. Molecules and Cells,34:53-59.
[76]	Lim H, Hwang H, Kim T, Kim S, Chung H, Lee D, Kim S, Park S, Cho W, Ji H, Lee G. 2021. Transcriptomic analysis of rice plants overexpressing PsGAPDH in response to salinity stress. Genes, 12 (5):641.
[77]	Lin M, Yan J, Ali M M, Wang S, Tian S, Chen F, Lin Z. 2022. Isolation and functional characterization of a green-tissue promoter in japonica rice (Oryza sativa subsp. Japonica). Biology, 11 (8):1092.
[78]	Liu B, Zhang B, Yang Z, Liu Y, Yang S, Shi Y, Jiang C, Qin F. 2021a. Manipulating ZmEXPA4 expression ameliorates the drought-induced prolonged anthesis and silking interval in maize. The Plant Cell, 33 (6):2058-2071.
[79]	Liu L, Gallagher J, Arevalo E D, Chen R, Skopelitis T, Wu Q, Bartlett M, Jackson D. 2021b. Enhancing grain-yield-related traits by CRISPR-Cas 9 promoter editing of maize CLE genes. Nature Plants, 7 (3):287-294.
[80]	Liu S, Liu C, Wang X, Chen H. 2021c. Seed-specific activity of the Arabidopsis β-glucosidase 19 promoter in transgenic Arabidopsis and tobacco. Plant Cell Reports,40:213-221.
[81]	Liu X, Ma X, Wang H, Li S, Yang W, Nugroho R D, Luo L, Zhou X, Tang C, Fan Y, Zhao Q, Zhang J, Chen R. 2021d. Metabolic engineering of astaxanthin-rich maize and its use in the production of biofortified eggs. Plant Biotechnology Journal, 19 (9):1812-1823.
[82]	Liu Y G, Chen Y. 2007. High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. Biotechniques, 43 (5):649-656.
[83]	Liu Z B, Ulmasov T, Shi X, Hagen G, Guilfoyle T J. 1994. Soybean GH3 promoter contains multiple auxin-inducible elements. The Plant Cell, 6 (5):645-657.
[84]	Ly L K, Bui T P, Le A V T, Nguyen P V, Ong P X, Pham N B, Zhang Z J, Do P T, Chu H H. 2022. Enhancing plant growth and biomass production by overexpression of GA20ox gene under control of a root preferential promoter. Transgenic Research, 31 (1):73-85.
[85]	Maiti S, Patro S, Pal A, Dey N. 2015. Identification of a novel salicylic acid inducible endogenous plant promoter regulating expression of CYR1,a CC-NB-LRR type candidate disease resistance gene in Vigna mungo. Plant Cell,Tissue and Organ Culture(PCTOC),120:489-505.
[86]	McElroy D, Blowers A D, Jenes B, Wu R. 1991. Construction of expression vectors based on the rice actin 1(Act1)5’region for use in monocot transformation. Molecular and General Genetics MGG,231:150-160.
[87]	Mohan C, Jayanarayanan A N, Narayanan S. 2017. Construction of a novel synthetic root-specific promoter and its characterization in transgenic tobacco plants. 3 Biotech,7:1-9.
[88]	Molla K A, Karmakar S, Chanda P K, Ghosh S, Sarkar S N, Datta S K, Datta K. 2013. Rice oxalate oxidase gene driven by green tissue-specific promoter increases tolerance to sheath blight pathogen(Rhizoctonia solani)in transgenic rice. Molecular Plant Pathology, 14 (9):910-922.
[89]	Montgomery J, Goldman S, Deikman J, Margossian L, Fischer R L. 1993a. Identification of an ethylene-responsive region in the promoter of a fruit ripening gene. Proceedings of the National Academy of Sciences, 90 (13):5939-5943.
[90]	Montgomery J, Pollard V, Deikman J, Fischer R L. 1993b. Positive and negative regulatory regions control the spatial distribution of polygalacturonase transcription in tomato fruit pericarp. The Plant Cell, 5 (9):1049-1062.
[91]	Negi N, Khurana P. 2021. A salicylic acid inducible mulberry WRKY transcription factor,MiWRKY 53 is involved in plant defence response. Plant Cell Reports,40:2151-2171.
[92]	Nielsen J, Keasling J D. 2016. Engineering cellular metabolism. Cell, 164 (6):1185-1197.
[93]	Nitz I, Berkefeld H, Puzio P S, Grundler F M. 2001. Pyk10,a seedling and root specific gene and promoter from Arabidopsis thaliana. Plant Science, 161 (2):337-346.
[94]	Odell J T, Nagy F, Chua N H. 1985. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature, 313 (6005):810-812.
[95]	Ohme-Takagi M, Shinshi H. 1995. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. The Plant Cell, 7 (2):173-182.
[96]	Oliva R, Ji C, Atienza-Grande G, Huguet-Tapia J C, Perez-Quintero A, Li T, Eom J, Li C, Nguyen H, Liu B, Auguy F, Sciallano C, Luu V T, Dossa G S, Cunnac S, Schmidt S M, Slamet-Loedin I H, Cruz C V, Szurek B, Frommer W B, White F F, Yang B. 2019. Broad-spectrum resistance to bacterial blight in rice using genome editing. Nature Biotechnology, 37 (11):1344-1350.
[97]	Pellizzaro A, Neveu M, Lalanne D, Ly Vu B, Kanno Y, Seo M, Leprince O, Buitink J. 2020. A role for auxin signaling in the acquisition of longevity during seed maturation. New Phytologist, 225 (1):284-296.
[98]	Qian D, Zhang Z, He J, Zhang P, Ou X, Li T, Niu L, Nan Q, Niu Y, He W, An L, Jiang K, Xiang Y. 2019. Arabidopsis ADF 5 promotes stomatal closure by regulating actin cytoskeleton remodeling in response to ABA and drought stress. Journal of Experimental Botany, 70 (2):435-446.
[99]	Quaedvlieg N E, Schlaman H R, Admiraal P C, Wijting S E, Stougaard J, Spaink H P. 1998. Fusions between green fluorescent protein and β-glucuronidase as sensitive and vital bifunctional reporters in plants. Plant Molecular Biology,38:861-873.
[100]	Rahman F U, Khan I A, Aslam A, Liu R, Sun L, Wu Y, Aslam M M, Khan A U, Li P, Jiang J, Fan X, Liu C, Zhang Y. 2022a. Transcriptome analysis reveals pathogenesis-related gene 1 pathway against salicylic acid treatment in grapevine(Vitis vinifera L). Frontiers in Genetics,13:1033288.
[101]	Rahman F U, Zhang Y, Khan I A, Liu R, Sun L, Wu Y, Jiang J, Fan X, Liu C. 2022b. The promoter analysis of VvPR1 gene:A candidate gene identified through transcriptional profiling of methyl jasmonate treated grapevine(Vitis vinifera L.). Plants, 11 (12):1540.
[102]	Reidt W, Wohlfarth T, Ellerström M, Czihal A, Tewes A, Ezcurra I, Rask L, Bäumlein H. 2000. Gene regulation during late embryogenesis:the RY motif of maturation-specific gene promoters is a direct target of the FUS3 gene product. The Plant Journal, 21 (5):401-408.
[103]	Ren C, Liu Y, Guo Y, Duan W, Fan P, Li S, Liang Z. 2021. Optimizing the CRISPR/Cas 9 system for genome editing in grape by using grape promoters. Horticulture Research, 8 (1):52.
[104]	Rocher A, Dumas C, Cock J M. 2005. A W-box is required for full expression of the SA-responsive gene SFR2. Gene,344:181-192.
[105]	Rogers J C, Rogers S W. 1992. Definition and functional implications of gibberellin and abscisic acid cis-acting hormone response complexes. The Plant Cell, 4 (11):1443-1451.
[106]	Roque E, Gómez-Mena C, Hamza R, Beltrán J P, Cañas L A. 2019. Engineered male sterility by early anther ablation using the pea anther-specific promoter PsEND1. Frontiers in Plant Science,10:461361.
[107]	Ruan X M, Xiong X, Li J F. 2024. Identification and application of an exocarp-preferential promoter for genetic engineering of tomato fruit. Horticulture Research, 11 (3):uhae035.
[108]	Rushton P J. 2016. What have we learned about synthetic promoter construction? Plant Synthetic Promoters: Methods and Protocols,1482:1-13.
[109]	Sarkar S, Das A, Khandagale P, Maiti I B, Chattopadhyay S, Dey N. 2018. Interaction of Arabidopsis TGA3 and WRKY 53 transcription factors on Cestrum yellow leaf curling virus(CmYLCV) promoter mediates salicylic acid-dependent gene expression in planta. Planta,247:181-199.
[110]	Singh S, Yadav S, Singh A, Mahima M, Singh A, Gautam V, Sarkar A K. 2020. Auxin signaling modulates LATERAL ROOT PRIMORDIUM1(LRP1)expression during lateral root development in Arabidopsis. The Plant Journal, 101 (1):87-100.
[111]	Skriver K, Olsen F L, Rogers J C, Mundy J. 1991. cis-Acting DNA elements responsive to gibberellin and its antagonist abscisic acid. Proceedings of the National Academy of Sciences, 88 (16):7266-7270.
[112]	Smale S T. 2001. Core promoters:active contributors to combinatorial gene regulation. Genes & Development, 15 (19):2503-2508.
[113]	Song S, Wang G, Wu H, Fan X, Liang L, Zhao H, Li S, Hu Y, Liu H, Ayaad M, Xing Y. 2020. OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice. The Plant Journal, 103 (2):532-546.
[114]	Sun C, Palmqvist S, Olsson H, Borén M, Ahlandsberg S, Jansson C. 2003. A novel WRKY transcription factor,SUSIBA2,participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter. The Plant Cell, 15 (9):2076-2092.
[115]	Sun S R, Wu X B, Chen J S, Huang M T, Fu H Y, Wang Q N, Rott P, Gao S J. 2024. Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants. Communications Biology, 7 (1):368.
[116]	Sun X M, Xiong H Y, Jiang C H, Zhang D M, Yang Z L, Huang Y P, Zhu W B, Ma S S, Duan J Z, Wang X, Liu W, Guo H F, Li G L, Qi J W, Liang C B, Zhang Z Y, Li J J, Zhang H L, Han L J, Zhou Y H, Peng Y L, Li Z C. 2022. Natural variation of DROT1 confers drought adaptation in upland rice. Nature Communications, 13 (1):4265.
[117]	Sutoh K, Yamauchi D. 2003. Two cis-acting elements necessary and sufficient for gibberellin-upregulated proteinase expression in rice seeds. The Plant Journal, 34 (5):635-645.
[118]	Szabała B M. 2023. A bifunctional selectable marker for wheat transformation contributes to the characterization of male-sterile phenotype induced by a synthetic Ms2 gene. Plant Cell Reports, 42 (5):895-907.
[119]	Tang H, Liu H, Zhou Y, Liu H, Du L, Wang K, Ye X. 2021. Fertility recovery of wheat male sterility controlled by Ms2 using CRISPR/Cas9. Plant Biotechnology Journal, 19 (2):224.
[120]	Tang W, Luo X, Samuels V. 2004. Regulated gene expression with promoters responding to inducers. Plant Science, 166 (4):827-834.
[121]	Thurow C, Schiermeyer A, Krawczyk S, Butterbrodt T, Nickolov K, Gatz C. 2005. Tobacco bZIP transcription factor TGA2. 2 and related factor TGA2. 1 have distinct roles in plant defense responses and plant development. The Plant Journal, 44 (1):100-113.
[122]	Tian L, Wu K, Hannam C, Latoszek-Green M, Sibbald S, Hu M, Brown D C W, Miki B. 2005. Analysis and use of the tobacco eIF4A-10 promoter elements for transgene expression. Journal of Plant Physiology, 162 (12):1355-1366.
[123]	Tounosu N, Sesoko K, Hori K, Shimojima M, Ohta H. 2023. cis-Regulatory elements and transcription factors related to auxin signaling in the streptophyte algae Klebsormidium nitens. Scientific Reports, 13 (1):9635.
[124]	Vallarino J G, Kubiszewski-Jakubiak S, Ruf S, Rößner M, Timm S, Bauwe H, Carrari F, Rentsch D, Bock R, Sweetlove L J, FernieA R. 2020. Multi-gene metabolic engineering of tomato plants results in increased fruit yield up to 23%. Scientific Reports, 10 (1):17219.
[125]	van Haaren M J J, Houck C M. 1991. Strong negative and positive regulatory elements contribute to the high-level fruit-specific expression of the tomato 2A11 gene. Plant Molecular Biology,17:615-630.
[126]	van Haaren M J J, Houck C M. 1993. A functional map of the fruit-specific promoter of the tomato 2A11 gene. Plant Molecular Biology,21:625-640.
[127]	Vaucheret H, Béclin C, Elmayan T, Feuerbach F, Godon C, Morel J B, Mourrain P, Palauqui J C, Vernhettes S. 1998. Transgene-induced gene silencing in plants. The Plant Journal, 16 (6):651-659.
[128]	Walcher C L, Nemhauser J L. 2012. Bipartite promoter element required for auxin response. Plant Physiology, 158 (1):273-282.
[129]	Wan L, Zhang J, Zhang H, Zhang Z, Quan R, Zhou S, Huang R. 2011. Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice. PLoS ONE, 6 (9):e25216.
[130]	Wang C, Li W, Chen F, Cheng Y, Huang X, Zou B, Wang Y, Xu W, Qu S. 2022. Genome-wide identification and characterization of members of the ACS gene family in Cucurbita maxima and their transcriptional responses to the specific treatments. International Journal of Molecular Sciences, 23 (15):8476.
[131]	Wang J X, Ming X, Tao Y B, Xu Z F. 2020a. Jatropha curcas ortholog of tomato MADS-box gene 6(JcTM6)promoter exhibits floral-specific activity in Arabidopsis thaliana. PeerJ,8:e9827.
[132]	Wang M, Yan W, Peng X, Chen Z, Xu C, Wu J, Deng X Y, Tang X. 2020b. Identification of late-stage pollen-specific promoters for construction of pollen-inactivation system in rice. Journal of Integrative Plant Biology, 62 (8):1246-1263.
[133]	Wang X, Replogle A M Y, Davis E L, Mitchum M G. 2007a. The tobacco Cel7 gene promoter is auxin-responsive and locally induced in nematode feeding sites of heterologous plants. Molecular Plant Pathology, 8 (4):423-436.
[134]	Wang Y Q, Zhu S Y, Wang Y, Zhang M Y. 2007b. Tissue and inducible expression of a rice glutathione transporter gene promoter in transgenic Arabidopsis. Bot Stud,48:35-41.
[135]	Welsch R, Wust F, Bar C, Al-Babili S, Beyer P. 2008. A third phytoene synthase is devoted to abiotic stress-induced abscisic acid formation in rice and defines functional diversification of phytoene synthase genes. Plant Physiology, 147 (1):367-380.
[136]	Wichmann J, Behrendt G, Boecker S, Klamt S. 2023. Characterizing and utilizing oxygen-dependent promoters for efficient dynamic metabolic engineering. Metabolic Engineering,77:199-207.
[137]	Wiley S R, KRAus R J, Mertz J E. 1992. Functional binding of the“TATA”box binding component of transcription factor TFIID to the-30 region of TATA-less promoters. Proceedings of the National Academy of Sciences, 89 (13):5814-5818.
[138]	Wu A M, Lv S Y, Liu J Y. 2007. Functional analysis of a cotton glucuronosyltransferase promoter in transgenic tobaccos. Cell Research, 17 (2):174-183.
[139]	Wu C Y, Washida H, Onodera Y, Harada K, Takaiwa F. 2000. Quantitative nature of the Prolamin-box,ACGT and AACA motifs in a rice glutelin gene promoter:minimal cis-element requirements for endosperm-specific gene expression. The Plant Journal, 23 (3):415-421.
[140]	Wu J G, Yang Z R, Wang Y, Zheng L J, Ye R Q, Ji Y H, Zhao S S, Ji S Y, Liu R F, Xu L, Zheng H, Zhou Y J, Zhang X, Cao X F, Xie L H, Wu Z J. 2015. Viral-inducible argonaute 18 confers broad-spectrum virus resistance in rice by sequestering a host microRNA. Elife,4:e05733.
[141]	Wu P, Xu X, Li J, Zhang J, Chang S, Yang X, Guo X. 2021. Seed-specific overexpression of cotton GhDGAT1 gene leads to increased oil accumulation in cottonseed. The Crop Journal, 9 (2):487-490.
[142]	Xia C, Zhang L C, Zou C, Gu Y Q, Duan J L, Zhao G Y, Wu J J, Liu Y, Fang X H, Gao L F, Jiao Y N, Sun J Q, Pan Y H, Liu X, Jia J Z, Kong X Y. 2017. A TRIM insertion in the promoter of Ms2 causes male sterility in wheat. Nature Communications, 8 (1):15407.
[143]	Xu H, Wang H, Zhang Y, Yang X, Lv S, Hou D, Mo C, Wassie M, Yu B, Hu T. 2023. A synthetic light-inducible photorespiratory bypass enhances photosynthesis to improve rice growth and grain yield. Plant Communications, 4 (6):100641.
[144]	Xu R, Goldman S, Coupe S, Deikman J. 1996. Ethylene control of E4 transcription during tomato fruit ripening involves two cooperative cis- elements. Plant Molecular Biology,31:1117-1127.
[145]	Xu Z S, Yang Q Q, Feng K, Yu X, Xiong A S. 2020. DcMYB113,a root-specific R2R3-MYB,conditions anthocyanin biosynthesis and modification in carrot. Plant Biotechnology Journal, 18 (7):1585-1597.
[146]	Xun H, Zhang X, Yu J, Pang J, Wang S, Liu B, Dong H, Jiang L, Guo D. 2021. Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean. Transgenic Research,30:799-810.
[147]	Yadav V K, Yadav V K, Pant P, Singh S P, Maurya R, Sable A, Sawant S V. 2017. GhMYB1 regulates SCW stage-specific expression of the GhGDSL promoter in the fibres of Gossypium hirsutum L. Plant Biotechnology Journal, 15 (9):1163-1174.
[148]	Yan L, Wei S, Wu Y, Hu R, Li H, Yang W, Xie Q. 2015. High-efficiency genome editing in Arabidopsis using YAO promoter-driven CRISPR/Cas 9 system. Molecular Plant, 8 (12):1820-1823.
[149]	Yang Jiangtao, Pang Weimin, Wang Xujing, Lü Shaofu, Tang Qiaoling, Wang Zhixing. 2016. Cloning and deletion analysis of GhRACK1 promoter from Gossypium hirsutum. Acta Agronomica Sinica, 42 (3):368-375. (in Chinese)
	杨江涛, 庞伟民, 王旭静, 吕少溥, 唐巧玲, 王志兴. 2016. 陆地棉GhRACK1启动子的克隆与缺失分析. 作物学报, 42 (3):368-375.
[150]	Yang Y Z, Tan B C. 2014. A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis. PLoS ONE, 9 (1):e87283.
[151]	Yuan C, Li C, Yan C, Zhao X, Wang J, Sun Q, Shan S. 2019. Isolation and characterization of a novel seed-specific promoter from peanut(Arachis hypogaea L.). Molecular Biology Reports,46:3183-3191.
[152]	Zerpa-Catanho D, Clough S J, Ming R. 2022. Characterization and analysis of the promoter region of monodehydroascorbate reductase 4(CpMDAR4)in papaya. Plant Reproduction, 35 (4):233-264.
[153]	Zhai X, Ji L, Gao J, Zhou Y J. 2021. Characterizing methanol metabolism-related promoters for metabolic engineering of Ogataea polymorpha. Applied Microbiology and Biotechnology,105:8761-8769.
[154]	Zhang C, Pan S, Chen H, Cai T, Zhuang C, Deng Y, Zhuang Y, Zeng Y, Chen S, Zhuang W. 2016. Characterization of NtREL1,a novel root-specific gene from tobacco,and upstream promoter activity analysis in homologous and heterologous hosts. Plant Cell Reports,35:757-769.
[155]	Zhang S, Li X, Fan S, Zhou L, Wang Y. 2020a. Overexpression of HcSCL13,a Halostachys caspica GRAS transcription factor,enhances plant growth and salt stress tolerance in transgenic Arabidopsis. Plant Physiology and Biochemistry,151:243-254.
[156]	Zhang S, Wu S, Hu C, Yang Q, Dong T, Sheng O, Deng G, He W, Dou T, Li C, Sun C, Yi G, Bi F. 2022. Increased mutation efficiency of CRISPR/Cas 9 genome editing in banana by optimized construct. PeerJ,10:e12664.
[157]	Zhang W, Wang N, Yang J, Guo H, Liu Z, Zheng X, Li S, Xiang F. 2020b. The salt-induced transcription factor GmMYB 84 confers salinity tolerance in soybean. Plant Science,291:110326.
[158]	Zhao Y, Sun Y, Huang S, Liu Z, Feng H. 2022. Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (Brassica rapa L. ssp. pekinensis). 3 Biotech,12 (4):104.
[159]	Zheng C, Zhang L, Tang Z, Zhao C, Yuan S. 2009. TAIL-PCR and its cloning in plant gene. Genomics and Applied Biology, 28 (3):544-548.
[160]	Zheng H, Lei Y, Lin S, Zhang Q, Zhang Z. 2011. Bidirectionalization of a methyl jasmonate-inducible plant promoter. Biotechnology Letters,33:387-393.
[161]	Zheng N, Li T, Dittman J D, Su J, Li R, Gassmann W, Peng D, Whitham S A, Liu S, Yang B. 2020. CRISPR/Cas9-based gene editing using egg cell-specific promoters in Arabidopsis and soybean. Frontiers in Plant Science,11:551133.
[162]	Zhou J, Liu G, Zhao Y, Zhang R, Tang X, Li L, Jia X, Guo Y, Wu Y, Han Y, Bao Y, He Y, Han Q, Yang H, Zheng X, Qi Y, Zhang T, Zhang Y. 2023. An efficient CRISPR-Cas12a promoter editing system for crop improvement. Nature Plants, 9 (4):588-604.
[163]	Zhou Y, Ma B, Tao J J, Yin C C, Hu Y, Huang Y H, Wei W, Xin P Y, Chu J F, Zhang W K, Chen S Y, Zhang J S. 2022. Rice EIL 1 interacts with OsIAAs to regulate auxin biosynthesis mediated by the tryptophan aminotransferase MHZ10/OsTAR2 during root ethylene responses. The Plant Cell, 34 (11):4366-4387.
[164]	Zou X, Song E, Peng A, He Y, Xu L, Lei T, Yao L, Chen S. 2014. Activation of three pathogen-inducible promoters in transgenic citrus(Citrus sinensis Osbeck)after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell,Tissue and Organ Culture,117:85-98.

启动子类型 Promoter type	来源基因 Source gene	顺式作用元件 cis-Acting element	来源物种 Source species	转化物种 Transformed species	报告基因 Reporter gene	参考文献 Reference
根特异性启动子 Root-specific promoter	OsGT1	ROOTMOTIFTAPOX1、POLLEN1LELAT52	水稻 Oryza sativa	拟南芥 Arabidopsis thaliana	GUS	Wang et al.,2007b
	GhRACK1	ROOTMOTIFTAPOX1、MYB2、TBOX、LIBOX、IBOXCORE	陆地棉Gossypium hirsutum	烟草Nicotiana tabacum	GUS	杨江涛等,2016
	DcMYB113	/	胡萝卜 Daucus carota subsp. sativus	胡萝卜 Daucus carota subsp. sativus	LUC	Xu et al.,2020
	At1g	/	拟南芥 Arabidopsis thaliana	烟草Nicotiana tabacum	GUS	Ly et al.,2022
	rRSP1	ROOTMOTIFTAPOX1、CACTFTPPCA1	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Huang et al.,2015
	rRSP3	ROOTMOTIFTAPOX1、CACTFTPPCA1	水稻 Oryza sativa	水稻 Oryza sativa	GUS
	rRSP5	ROOTMOTIFTAPOX1、CACTFTPPCA1、OSE1/2ROOTNODULE	水稻 Oryza sativa	水稻 Oryza sativa	GUS
	SynR2	ATATT、MAR-box	人工合成 Synthetic	烟草 Nicotiana tabacum	GUS	Mohan et al.,2017
	E543254P	RHERPATEXPA7、SP8BFIBSP8BIB、OSE1ROOTNODULE、WUSATAg	番茄 Solanum lycopersicum	拟南芥 Arabidopsis thaliana	GUS	Lim et al.,2012
	E542814P	RHERPATEXPA7、SP8BFIBSP8BIB、OSE1ROOTNODULE、WUSATAg	番茄 Solanum lycopersicum	拟南芥 Arabidopsis thaliana	GUS	Lim et al.,2012
	GmTIP	OSE2ROOTNODULE、ROOTMOTIFTAPOX1	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Chen et al.,2015
	GmADR1	M1-motif、M2-motif、M3-motif、RHE	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Xun et al.,2021
	GmBTP1	M1-motif、M2-motif 、M3-motif、RHE	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS
	pNtREL1	OSE1、OSE2、ROOTMOTIFTAPOX1、SURECOREATSULTR11、P1BS、WUSATAg	烟草 Nicotiana tabacum	烟草 Nicotiana tabacum	GUS	Zhang et al.,2016
	Pyk10	ocs-elements、mas-elements、as-1-elements	拟南芥 Arabidopsis thaliana	拟南芥Arabidopsis thaliana	GUS	Nitz et al.,2001
花器官特异启动子 Floral organ-specific promoter	PsEND1	/	豌豆Pisum sativum	拟南芥Arabidopsis thaliana	GUS	Roque et al.,2019
	BraA08g014780.3C	POLLEN1LELAT52、GTGANTG10	白菜Brassica rapa	拟南芥Arabidopsis thaliana	GUS	Zhao et al.,2022
	CpMDAR4	POLLEN1LELAT52、CREs	木瓜 Carica papaya	拟南芥 Arabidopsis thaliana	GUS	Zerpa-Catanho et al.,2022
	rMdAG2I	TATA-box	人工合成 Synthetic	烟草、苹果 Nicotiana tabacum、Malus domestica	GUS	Dong et al.,2019
	JcTM6	CArG-box、GTGA、AGAAA-motif	麻风树 Jatropha curcas	拟南芥 Arabidopsis thaliana	GUS	Wang et al.,2020a
	PSG076	/	小麦 Triticum aestivum	小麦 Triticum aestivum	GUS	Chen et al.,2023b
	OsLSP3	LAT enhancer element、TGGTTA-motif、AGAAA-motif、AGGTCA-motif	水稻 Oryza sativa	水稻 Oryza sativa	GFP	Wang et al.,2020b
	OsLSP5	ATATT-motif、TTTCT-motif、AGAAA-motif	水稻 Oryza sativa	水稻 Oryza sativa	GFP
	OsLSP6	TGTGG-motif、TGGTTA-motif、AGAAA-motif、TTTCT-motif、AGGTCA-motif、AAATGA-motif、ATATT-motif、GATAA-motif、TAAATC-motif	水稻 Oryza sativa	水稻 Oryza sativa	GFP
	CtFSG2	ARE-box、Box4、CGTCA-motif、LTR	红花 Carthamus tinctorius	拟南芥 Arabidopsis thaliana	GUS	Chen et al.,2023a
种子特异性启动子 Seed-specific promoter	AtBGLU19	RY-motif、SEF3、SEF4、ESP、2SSEEDPROTBANAPA	拟南芥 Arabidopsis thaliana	拟南芥、烟草 Arabidopsis thaliana、Nicotiana tabacum	GUS	Liu et al.,2021c
	AX795651	/	陆地棉 Gossypium hirsutum	陆地棉 Gossypium hirsutum	GhDGAT1
	Emb5	AGCCCA-motif、GAAAAA-motif、CACT-motif、GCAA-motif、ABRE、TCA、Skn-1-motif、G-box、E-box、P-box、RAA、ATTTTC-motif、TGAATG-motif	玉米 Zea mays	拟南芥Arabidopsis thaliana	GUS	Li et al.,2021
	AHSSP2	RY-motif、G-box、GT1-box、EBOXBNNAPA、SEF1、SEF4	花生 Arachis hypogaea	拟南芥Arabidopsis thaliana	GUS	Yuan et al.,2019
	VvβVPE	CATGCA-motif、CAAACAC-motif、E-box、G-box	葡萄 Vitis vinifera	拟南芥Arabidopsis thaliana	GUS	Gong et al.,2019
果实特异性启动子 Fruit-specific promoter	2A11	F-1、F-2、F-3、F-4、F-5、S-1、S-2、S-3	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	van Haaren & Houck,1991,1993
	E4、E8	/	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	LUC、GUS	Kneissl & Deikman,1996；Xu et al.,1996
	PG	/	番茄、苹果 Solanum lycopersicum、Malus × domestica	番茄 Solanum lycopersicum	GUS	Montgomery et al.,1993b
	SlPR10	/	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GFP-GUS	Ruan et al.,2024
	SlAdh2	MBs、Box4、ARE	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	Chung et al.,2020
	SlHDC-A	SBP-box、ATTTA-motif、GGATG-motif	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	Kim et al.,2019
	pSolyc03g098700.1、pSolyc04g007770.2、pSolyc07g049140.2、pSolyc10g075090.1	(CA)n、ERE、GASRE、ARE2	番茄 Solanum lycopersicum	/	/	Chen et al.,2021b
	CitSEP	ARE、HSE、Box4、GCN4 motif、I-box	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS	Dasgupta et al.,2020
	CitWAX	HSE、Skn-1 motif、I-box、TCA element	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS
	CitJuSac	Box4、HSE、ABRE、Box1	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS
	CitVO1	GARE-motif、Box1、HSE、ARE、ABER	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS
	PamMybAp	ARE、HSE、BOx4、as-2-box、MBS	李子 Prunus salicina	番茄 Solanum lycopersicum	GUS
	GSX7R	ACE、Box4、CAT box、G-Box、G-box、GATA motif、GC motif、I-box、Sp1、chs-unit1 M1	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Lin et al.,2022
	AraSSU	/	拟南芥 Arabidopsis thaliana	鹰嘴豆 Cicer arietinum	cry2Aa	Boruah et al.,2023
	BiGSSP2	POsrbcs-62、OsAct1、POsrbcs-550	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS	Bai et al.,2020
	BiGSSP3	POsrbcs-62、OsAct1、4×GEAT	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS
	BiGSSP7	POsrbcs-550、PD540-544、OsAct1、OsTub6I	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS
	BiGSSP6	OsAct1	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS
维管束特异性启动子 Vascular tissue-specific promoter	DROT1	/	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Sun et al.,2022
	SUC2	/	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Archer et al.,2023
	GmTIP1;6	MBS、ABRE、CGTCA-motif、TGACG-motif、TATC-box、TGA	毛豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Feng et al.,2022

启动子类型 Promoter type	来源基因 Source gene	顺式作用元件 cis-Acting element	来源物种 Source species	转化物种 Transformed species	报告基因 Reporter gene	参考文献 Reference
根特异性启动子 Root-specific promoter	OsGT1	ROOTMOTIFTAPOX1、POLLEN1LELAT52	水稻 Oryza sativa	拟南芥 Arabidopsis thaliana	GUS	Wang et al.,2007b
	GhRACK1	ROOTMOTIFTAPOX1、MYB2、TBOX、LIBOX、IBOXCORE	陆地棉Gossypium hirsutum	烟草Nicotiana tabacum	GUS	杨江涛等,2016
	DcMYB113	/	胡萝卜 Daucus carota subsp. sativus	胡萝卜 Daucus carota subsp. sativus	LUC	Xu et al.,2020
	At1g	/	拟南芥 Arabidopsis thaliana	烟草Nicotiana tabacum	GUS	Ly et al.,2022
	rRSP1	ROOTMOTIFTAPOX1、CACTFTPPCA1	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Huang et al.,2015
	rRSP3	ROOTMOTIFTAPOX1、CACTFTPPCA1	水稻 Oryza sativa	水稻 Oryza sativa	GUS
	rRSP5	ROOTMOTIFTAPOX1、CACTFTPPCA1、OSE1/2ROOTNODULE	水稻 Oryza sativa	水稻 Oryza sativa	GUS
	SynR2	ATATT、MAR-box	人工合成 Synthetic	烟草 Nicotiana tabacum	GUS	Mohan et al.,2017
	E543254P	RHERPATEXPA7、SP8BFIBSP8BIB、OSE1ROOTNODULE、WUSATAg	番茄 Solanum lycopersicum	拟南芥 Arabidopsis thaliana	GUS	Lim et al.,2012
	E542814P	RHERPATEXPA7、SP8BFIBSP8BIB、OSE1ROOTNODULE、WUSATAg	番茄 Solanum lycopersicum	拟南芥 Arabidopsis thaliana	GUS	Lim et al.,2012
	GmTIP	OSE2ROOTNODULE、ROOTMOTIFTAPOX1	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Chen et al.,2015
	GmADR1	M1-motif、M2-motif、M3-motif、RHE	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Xun et al.,2021
	GmBTP1	M1-motif、M2-motif 、M3-motif、RHE	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS
	pNtREL1	OSE1、OSE2、ROOTMOTIFTAPOX1、SURECOREATSULTR11、P1BS、WUSATAg	烟草 Nicotiana tabacum	烟草 Nicotiana tabacum	GUS	Zhang et al.,2016
	Pyk10	ocs-elements、mas-elements、as-1-elements	拟南芥 Arabidopsis thaliana	拟南芥Arabidopsis thaliana	GUS	Nitz et al.,2001
花器官特异启动子 Floral organ-specific promoter	PsEND1	/	豌豆Pisum sativum	拟南芥Arabidopsis thaliana	GUS	Roque et al.,2019
	BraA08g014780.3C	POLLEN1LELAT52、GTGANTG10	白菜Brassica rapa	拟南芥Arabidopsis thaliana	GUS	Zhao et al.,2022
	CpMDAR4	POLLEN1LELAT52、CREs	木瓜 Carica papaya	拟南芥 Arabidopsis thaliana	GUS	Zerpa-Catanho et al.,2022
	rMdAG2I	TATA-box	人工合成 Synthetic	烟草、苹果 Nicotiana tabacum、Malus domestica	GUS	Dong et al.,2019
	JcTM6	CArG-box、GTGA、AGAAA-motif	麻风树 Jatropha curcas	拟南芥 Arabidopsis thaliana	GUS	Wang et al.,2020a
	PSG076	/	小麦 Triticum aestivum	小麦 Triticum aestivum	GUS	Chen et al.,2023b
	OsLSP3	LAT enhancer element、TGGTTA-motif、AGAAA-motif、AGGTCA-motif	水稻 Oryza sativa	水稻 Oryza sativa	GFP	Wang et al.,2020b
	OsLSP5	ATATT-motif、TTTCT-motif、AGAAA-motif	水稻 Oryza sativa	水稻 Oryza sativa	GFP
	OsLSP6	TGTGG-motif、TGGTTA-motif、AGAAA-motif、TTTCT-motif、AGGTCA-motif、AAATGA-motif、ATATT-motif、GATAA-motif、TAAATC-motif	水稻 Oryza sativa	水稻 Oryza sativa	GFP
	CtFSG2	ARE-box、Box4、CGTCA-motif、LTR	红花 Carthamus tinctorius	拟南芥 Arabidopsis thaliana	GUS	Chen et al.,2023a
种子特异性启动子 Seed-specific promoter	AtBGLU19	RY-motif、SEF3、SEF4、ESP、2SSEEDPROTBANAPA	拟南芥 Arabidopsis thaliana	拟南芥、烟草 Arabidopsis thaliana、Nicotiana tabacum	GUS	Liu et al.,2021c
	AX795651	/	陆地棉 Gossypium hirsutum	陆地棉 Gossypium hirsutum	GhDGAT1
	Emb5	AGCCCA-motif、GAAAAA-motif、CACT-motif、GCAA-motif、ABRE、TCA、Skn-1-motif、G-box、E-box、P-box、RAA、ATTTTC-motif、TGAATG-motif	玉米 Zea mays	拟南芥Arabidopsis thaliana	GUS	Li et al.,2021
	AHSSP2	RY-motif、G-box、GT1-box、EBOXBNNAPA、SEF1、SEF4	花生 Arachis hypogaea	拟南芥Arabidopsis thaliana	GUS	Yuan et al.,2019
	VvβVPE	CATGCA-motif、CAAACAC-motif、E-box、G-box	葡萄 Vitis vinifera	拟南芥Arabidopsis thaliana	GUS	Gong et al.,2019
果实特异性启动子 Fruit-specific promoter	2A11	F-1、F-2、F-3、F-4、F-5、S-1、S-2、S-3	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	van Haaren & Houck,1991,1993
	E4、E8	/	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	LUC、GUS	Kneissl & Deikman,1996；Xu et al.,1996
	PG	/	番茄、苹果 Solanum lycopersicum、Malus × domestica	番茄 Solanum lycopersicum	GUS	Montgomery et al.,1993b
	SlPR10	/	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GFP-GUS	Ruan et al.,2024
	SlAdh2	MBs、Box4、ARE	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	Chung et al.,2020
	SlHDC-A	SBP-box、ATTTA-motif、GGATG-motif	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	Kim et al.,2019
	pSolyc03g098700.1、pSolyc04g007770.2、pSolyc07g049140.2、pSolyc10g075090.1	(CA)n、ERE、GASRE、ARE2	番茄 Solanum lycopersicum	/	/	Chen et al.,2021b
	CitSEP	ARE、HSE、Box4、GCN4 motif、I-box	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS	Dasgupta et al.,2020
	CitWAX	HSE、Skn-1 motif、I-box、TCA element	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS
	CitJuSac	Box4、HSE、ABRE、Box1	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS
	CitVO1	GARE-motif、Box1、HSE、ARE、ABER	柑橘 Citrus reticulata	番茄 Solanum lycopersicum	GUS
	PamMybAp	ARE、HSE、BOx4、as-2-box、MBS	李子 Prunus salicina	番茄 Solanum lycopersicum	GUS
	GSX7R	ACE、Box4、CAT box、G-Box、G-box、GATA motif、GC motif、I-box、Sp1、chs-unit1 M1	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Lin et al.,2022
	AraSSU	/	拟南芥 Arabidopsis thaliana	鹰嘴豆 Cicer arietinum	cry2Aa	Boruah et al.,2023
	BiGSSP2	POsrbcs-62、OsAct1、POsrbcs-550	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS	Bai et al.,2020
	BiGSSP3	POsrbcs-62、OsAct1、4×GEAT	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS
	BiGSSP7	POsrbcs-550、PD540-544、OsAct1、OsTub6I	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS
	BiGSSP6	OsAct1	人工合成 Synthetic	水稻 Oryza sativa	GFP、GUS
维管束特异性启动子 Vascular tissue-specific promoter	DROT1	/	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Sun et al.,2022
	SUC2	/	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Archer et al.,2023
	GmTIP1;6	MBS、ABRE、CGTCA-motif、TGACG-motif、TATC-box、TGA	毛豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Feng et al.,2022

激素Hormone	来源基因 Source gene	顺式作用元件cis-Acting element		来源物种 Source species	转化物种 Transformed species	报告基因 Reporter gene	参考文献 Reference
激素Hormone	来源基因 Source gene	名称 Name	作用 Function	来源物种 Source species	转化物种 Transformed species	报告基因 Reporter gene	参考文献 Reference
IAA	NtCel7	CATATG-motif	IAA反应元件IAA response element	烟草 Nicotiana tabacum	拟南芥 Arabidopsis thaliana	GUS	Wang et al.,2007a
		TATCCA-motif	GA反应元件GA response element
		W-box	SA、病原反应元件SA and pathogen response elements
		GT-1	病原体、盐胁迫pathogen、salt stress
	KnLBD1	RY-motif、CCTG-motif	IAA反应元件IAA response element	丝状绿藻 K. nitens	烟草 Nicotiana tabacum	GUS	Tounosu et al.,2023
	Dof5.8	ARF-bs	IAA反应因子结合位点IAA response factor binding site	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Konishi et al.,2015
	SlGH3.4	TGTCAC-motif	IAA反应元件IAA response element	番茄 Solanum lycopersicum	烟草 Nicotiana tabacum	GUS	Liao et al.,2015
	PIN1、PIN3、PIN7	AAGACA-motif、GAGACA-motif、DR5、GGGACA-motif	IAA反应元件 IAA response element	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Krogan et al.,2016
	LRP1	ARF7-bs、ARF9-bs	IAA反应因子结合位点IAA response factor binding site	拟南芥Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Singh et al., 2020
	10x2DR5-2DR5v2	DR5、DR5v2	IAA反应元件IAA response element	人工合成 Synthetic	拟南芥 Arabidopsis thaliana	GUS	Fernandez-Moreno et al.,2024
ABA	ADF5	ACGT/C	ABA响应元件核心基ABA-responsive element core motif	拟南芥Arabidopsis thaliana	烟草 Nicotiana tabacum	LUC	Qian et al.,2019
	Rab16A	G-box、Motif I	ABA响应元件 ABA response element	水稻 Oryza sativa	水稻 Oryza sativa	LUC	Song et al.,2020
	OsPSY3	G-box	ABA响应元件 ABA response element	水稻 Oryza sativa	/	/	Welsch et al.,2008
	OsPSY3	CE	干旱、盐胁迫响应元件 Drought and salt stress response elements	水稻 Oryza sativa	/	/	Welsch et al.,2008
	HvSnRK1	ABRE、LRE	ABA响应元件、光反应元件 ABA response elements、light response elements	大麦 Hordeum vulgare	/	/	Chen et al.,2021c
	SynP16	ACGTGTC-motif	like-ABA响应元件ABA-like response elements	人工合成 Synthetic	大豆 Glycine max	GUS	Jameel et al.,2020
		TACGTGGC-motif	ABA响应元件ABA response element
		GCC-box	ETH响应元件ETH response element
		Root--motif	根特异性元件root-specific elements
	GmRD26	CCACGTGG-motif、TCACGTGGC-motif、G-box、	ABA响应元件ABA response element	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Freitas et al.,2019
	GmRD26	DREB、MYC、MYB	干旱响应元件Drought response element	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Freitas et al.,2019
	AtNCED3	GGCACGTG-motif、GCCACGTC-motif、CACGTGGC-motif	ABA响应元件ABA response element	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Yang & Tan,2014
	PSCBV-YZ2060	TACGTG-motif、GCCACGT-motif	ABA响应元件ABA response element	甘蔗花叶病毒 SCMV	甘蔗 Saccharum officinarum	GUS	Sun et al.,2024
	osr40c1	GACAC-motif、G-box、GAGGC-motif、	ABA响应元件ABA response element	水稻 Oryza sativa	/	/	de Silva et al.,2017
		AAACAGA-motif	GA响应元件GA response element
		ATCT-motif、TGA-element、BoxI	光响应元件Light response elements
		Skn-1 motif	胚乳特异性表达Endosperm-specific expression
		Box E、Box-W1	真菌诱导Fungal induction
GA	Amy32b	GTAACAGAGTCTGG-motif	GA响应元件GA response elements	大麦02SHordeum vulgare	大麦02SHordeum vulgare	GUS	Rogers & Rogers,1992
	Amy32b	02S	耦合元件Coupling elements	大麦02SHordeum vulgare	大麦02SHordeum vulgare	GUS	Rogers & Rogers,1992
	GhGlcAT1	MYB1AT、MYC	MYB 结合基序MYB binding motif、干旱响应元件Drought response elements	陆地棉Gossypium hirsutum	陆地棉Gossypium hirsutum	GUS	Yadav et al.,2017
	Rep1	TAACAGA-motif、CAACTCCC-motif、CAACTACAC-motif、AAGACAAT-motif	GA响应元件GA response elements	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Sutoh &Yamauchi,2003
	GmGASA32	/	/	大豆 Glycine max	大豆 Glycine max	GUS	Chen et al.,2021a
ETH	E4、E8	/	/	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	Kneissl & Deikman,1996；Xu et al.,1996
	10x2EBS-S10	2EBS-S10	/	合成 Synthetic	拟南芥 Arabidopsis thaliana	GUS	Fernandez-Moreno et al.,2024
	CmaACS	ERE、W-box	ETH反应元件ETH response elements	南瓜 Cucurbita pepo	/	/	Wang et al.,2022
		TCA-element	SA反应元件SA response elements
ETH	CmaACS	ABRE	ABA反应元件ABA response elements	南瓜 Cucurbita pepo	/	/	Wang et al.,2022
		GARE	GA反应元件GA response elements
		ARE、LTR、MBS	应激反应元件Stress response elements
	OsTAR2	EBS-element	ETH响应元件ETH response elements	水稻 Oryza sativa	烟草、水稻 Nicotiana tabacum，Oryza sativa	LUC、GUS	Zhou et al.,2022
SA	SFR2	W-box	SA诱导元件SA-inducible elements	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Rocher et al.,2005
SA	FUASCsV8CP	/	/	合成 Synthetic	烟草 Nicotiana tabacum	GUS	Deb & Dey,2019
	Cyr1P4、Cyr1P5	TCA-element、WRKY71OS、WBOXATNPR1	SA诱导元件SA-inducible elements	黄花叶病毒 Cucumber mosaic virus	烟草、菠菜、洋葱、绿豆 Nicotiana tabacum，Spinacia oleracea，Allium cepa，Vigna radiata	GUS	Maiti et al.,2015
		MYB1AT、MYBCORE、MYCCONSENSUSA	响应干旱、ABA诱导Responsive to drought and ABA induction
		TAAG-motif、YACTT	叶肉细胞特异性元件Mesophyll-specific elements
	CmYLCV	as-1、W-box1	SA诱导顺式元件SA-inducible cis-elements	夜来香黄叶卷曲病毒 MJCMV	烟草、拟南芥 Nicotiana tabacum，Arabidopsis thaliana	GUS	Sarkar et al.,2018
	VqMYB15	MRE、Box Ⅰ、Box4、LAMP-element	光敏元件Photosensitive elements	葡萄 Vitis vinifera	烟草、葡萄 Nicotiana tabacum，Vitis vinifera	GUS	Li et al.,2020b
		TATTTTTCTC-motif、CCATCTATTT-motif、GAGAAGAAGA-motif	SA反应元件 SA response elements
		ABRE、MYC、CAATTG-motif	干旱、ABA诱导元件Drought and ABA-inducible elements
	D100	DRE core、GT1-box、MYC、MYCR、as-1	响应环境胁迫Responsive to environmental stress	大丽花花叶病毒 DMV	烟草 Nicotiana tabacum	GUS	Khadanga et al.,2021
	FvTrihelix6	G-box、ARE、MBS、LTR	应激响应元件 Stress response elements	草莓 Fragaria × ananassa	烟草 Nicotiana tabacum	GUS	Fan et al.,2023
	FvTrihelix6	ABRE	ABA响应元件 ABA response element	草莓 Fragaria × ananassa	烟草 Nicotiana tabacum	GUS	Fan et al.,2023
	MiWRKY53	W-box、ERE	ETH反应元件 ETH response element	桑树 Morus alba	拟南芥 Arabidopsis thaliana	GUS	Negi & Khurana,2021
		TCA-element	SA响应元件 SA response element
		ABRERATCAL、ACGTG-motif、TACGTG-motif、ACCGAC-motif	ABA响应元件 ABA response element
		TGA-element	IAA响应元件 IAA response element
		MYC	干旱响应元件 Drought response elements
JA	CYP81D11	TGACGTCA-motif	结合TGA转录因子Binding to TGA transcription factors	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Köster et al.,2012
	CYP81D11	as-1、G-box	结合JA调节的转录因子Transcription factors regulated by JA binding	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Köster et al.,2012
	TSA1	/	/	拟南芥 Arabidopsis thaliana	拟南芥、烟草 Arabidopsis thaliana，Nicotiana tabacum	GFP	Geem et al.,2019
	Zmap	CGTCA-motif、TGACG-motif	MeJA响应元件 MeJA response elements	玉米 Zea mays	烟草 Nicotiana tabacum	GUS	Jin et al.,2019
		GGTCCAT-motif	IAA响应元件 IAA response elements
		Box-II、GA-motif	光响应元件 Light response elements
		H-box、MBS	ABA、干旱响应元件ABA and drought response elements
	VvPR1	ACGTG-motif、MBS	ABA、干旱响应响应元件 ABA and drought response elements	葡萄 Vitis vinifera	烟草 Nicotiana tabacum	GUS	Rahman et al.,2022b
		ERE	ETH响应元件					ETH and drought response elements
		P-box	GA响应元件 GA response elements
		TCA	SA反应元件 SA response elements
		CGTCA-motif	MeJA响应元件 MeJA response elements
		LS7、G-box、AT1-motif	光响应元件 Light response elements
	TkSRPP	CGTCA-motif	MeJA响应元件 MeJA response elements	蒲公英 Taraxacum officinale	烟草 Nicotiana tabacum	GUS	Dong et al.,2023
		TGA-element	IAA响应元件 IAA response elements
		W-box、TTGAC-motif	SA响应元件 SA response elements
		CANNTG-motif、CNGTTR-motif、ACTG-motif	ABA响应元件 GA response elements
		MBS	干旱响应 Drought response

激素Hormone	来源基因 Source gene	顺式作用元件cis-Acting element		来源物种 Source species	转化物种 Transformed species	报告基因 Reporter gene	参考文献 Reference
激素Hormone	来源基因 Source gene	名称 Name	作用 Function	来源物种 Source species	转化物种 Transformed species	报告基因 Reporter gene	参考文献 Reference
IAA	NtCel7	CATATG-motif	IAA反应元件IAA response element	烟草 Nicotiana tabacum	拟南芥 Arabidopsis thaliana	GUS	Wang et al.,2007a
		TATCCA-motif	GA反应元件GA response element
		W-box	SA、病原反应元件SA and pathogen response elements
		GT-1	病原体、盐胁迫pathogen、salt stress
	KnLBD1	RY-motif、CCTG-motif	IAA反应元件IAA response element	丝状绿藻 K. nitens	烟草 Nicotiana tabacum	GUS	Tounosu et al.,2023
	Dof5.8	ARF-bs	IAA反应因子结合位点IAA response factor binding site	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Konishi et al.,2015
	SlGH3.4	TGTCAC-motif	IAA反应元件IAA response element	番茄 Solanum lycopersicum	烟草 Nicotiana tabacum	GUS	Liao et al.,2015
	PIN1、PIN3、PIN7	AAGACA-motif、GAGACA-motif、DR5、GGGACA-motif	IAA反应元件 IAA response element	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Krogan et al.,2016
	LRP1	ARF7-bs、ARF9-bs	IAA反应因子结合位点IAA response factor binding site	拟南芥Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Singh et al., 2020
	10x2DR5-2DR5v2	DR5、DR5v2	IAA反应元件IAA response element	人工合成 Synthetic	拟南芥 Arabidopsis thaliana	GUS	Fernandez-Moreno et al.,2024
ABA	ADF5	ACGT/C	ABA响应元件核心基ABA-responsive element core motif	拟南芥Arabidopsis thaliana	烟草 Nicotiana tabacum	LUC	Qian et al.,2019
	Rab16A	G-box、Motif I	ABA响应元件 ABA response element	水稻 Oryza sativa	水稻 Oryza sativa	LUC	Song et al.,2020
	OsPSY3	G-box	ABA响应元件 ABA response element	水稻 Oryza sativa	/	/	Welsch et al.,2008
	OsPSY3	CE	干旱、盐胁迫响应元件 Drought and salt stress response elements	水稻 Oryza sativa	/	/	Welsch et al.,2008
	HvSnRK1	ABRE、LRE	ABA响应元件、光反应元件 ABA response elements、light response elements	大麦 Hordeum vulgare	/	/	Chen et al.,2021c
	SynP16	ACGTGTC-motif	like-ABA响应元件ABA-like response elements	人工合成 Synthetic	大豆 Glycine max	GUS	Jameel et al.,2020
		TACGTGGC-motif	ABA响应元件ABA response element
		GCC-box	ETH响应元件ETH response element
		Root--motif	根特异性元件root-specific elements
	GmRD26	CCACGTGG-motif、TCACGTGGC-motif、G-box、	ABA响应元件ABA response element	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Freitas et al.,2019
	GmRD26	DREB、MYC、MYB	干旱响应元件Drought response element	大豆 Glycine max	拟南芥 Arabidopsis thaliana	GUS	Freitas et al.,2019
	AtNCED3	GGCACGTG-motif、GCCACGTC-motif、CACGTGGC-motif	ABA响应元件ABA response element	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Yang & Tan,2014
	PSCBV-YZ2060	TACGTG-motif、GCCACGT-motif	ABA响应元件ABA response element	甘蔗花叶病毒 SCMV	甘蔗 Saccharum officinarum	GUS	Sun et al.,2024
	osr40c1	GACAC-motif、G-box、GAGGC-motif、	ABA响应元件ABA response element	水稻 Oryza sativa	/	/	de Silva et al.,2017
		AAACAGA-motif	GA响应元件GA response element
		ATCT-motif、TGA-element、BoxI	光响应元件Light response elements
		Skn-1 motif	胚乳特异性表达Endosperm-specific expression
		Box E、Box-W1	真菌诱导Fungal induction
GA	Amy32b	GTAACAGAGTCTGG-motif	GA响应元件GA response elements	大麦02SHordeum vulgare	大麦02SHordeum vulgare	GUS	Rogers & Rogers,1992
	Amy32b	02S	耦合元件Coupling elements	大麦02SHordeum vulgare	大麦02SHordeum vulgare	GUS	Rogers & Rogers,1992
	GhGlcAT1	MYB1AT、MYC	MYB 结合基序MYB binding motif、干旱响应元件Drought response elements	陆地棉Gossypium hirsutum	陆地棉Gossypium hirsutum	GUS	Yadav et al.,2017
	Rep1	TAACAGA-motif、CAACTCCC-motif、CAACTACAC-motif、AAGACAAT-motif	GA响应元件GA response elements	水稻 Oryza sativa	水稻 Oryza sativa	GUS	Sutoh &Yamauchi,2003
	GmGASA32	/	/	大豆 Glycine max	大豆 Glycine max	GUS	Chen et al.,2021a
ETH	E4、E8	/	/	番茄 Solanum lycopersicum	番茄 Solanum lycopersicum	GUS	Kneissl & Deikman,1996；Xu et al.,1996
	10x2EBS-S10	2EBS-S10	/	合成 Synthetic	拟南芥 Arabidopsis thaliana	GUS	Fernandez-Moreno et al.,2024
	CmaACS	ERE、W-box	ETH反应元件ETH response elements	南瓜 Cucurbita pepo	/	/	Wang et al.,2022
		TCA-element	SA反应元件SA response elements
ETH	CmaACS	ABRE	ABA反应元件ABA response elements	南瓜 Cucurbita pepo	/	/	Wang et al.,2022
		GARE	GA反应元件GA response elements
		ARE、LTR、MBS	应激反应元件Stress response elements
	OsTAR2	EBS-element	ETH响应元件ETH response elements	水稻 Oryza sativa	烟草、水稻 Nicotiana tabacum，Oryza sativa	LUC、GUS	Zhou et al.,2022
SA	SFR2	W-box	SA诱导元件SA-inducible elements	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Rocher et al.,2005
SA	FUASCsV8CP	/	/	合成 Synthetic	烟草 Nicotiana tabacum	GUS	Deb & Dey,2019
	Cyr1P4、Cyr1P5	TCA-element、WRKY71OS、WBOXATNPR1	SA诱导元件SA-inducible elements	黄花叶病毒 Cucumber mosaic virus	烟草、菠菜、洋葱、绿豆 Nicotiana tabacum，Spinacia oleracea，Allium cepa，Vigna radiata	GUS	Maiti et al.,2015
		MYB1AT、MYBCORE、MYCCONSENSUSA	响应干旱、ABA诱导Responsive to drought and ABA induction
		TAAG-motif、YACTT	叶肉细胞特异性元件Mesophyll-specific elements
	CmYLCV	as-1、W-box1	SA诱导顺式元件SA-inducible cis-elements	夜来香黄叶卷曲病毒 MJCMV	烟草、拟南芥 Nicotiana tabacum，Arabidopsis thaliana	GUS	Sarkar et al.,2018
	VqMYB15	MRE、Box Ⅰ、Box4、LAMP-element	光敏元件Photosensitive elements	葡萄 Vitis vinifera	烟草、葡萄 Nicotiana tabacum，Vitis vinifera	GUS	Li et al.,2020b
		TATTTTTCTC-motif、CCATCTATTT-motif、GAGAAGAAGA-motif	SA反应元件 SA response elements
		ABRE、MYC、CAATTG-motif	干旱、ABA诱导元件Drought and ABA-inducible elements
	D100	DRE core、GT1-box、MYC、MYCR、as-1	响应环境胁迫Responsive to environmental stress	大丽花花叶病毒 DMV	烟草 Nicotiana tabacum	GUS	Khadanga et al.,2021
	FvTrihelix6	G-box、ARE、MBS、LTR	应激响应元件 Stress response elements	草莓 Fragaria × ananassa	烟草 Nicotiana tabacum	GUS	Fan et al.,2023
	FvTrihelix6	ABRE	ABA响应元件 ABA response element	草莓 Fragaria × ananassa	烟草 Nicotiana tabacum	GUS	Fan et al.,2023
	MiWRKY53	W-box、ERE	ETH反应元件 ETH response element	桑树 Morus alba	拟南芥 Arabidopsis thaliana	GUS	Negi & Khurana,2021
		TCA-element	SA响应元件 SA response element
		ABRERATCAL、ACGTG-motif、TACGTG-motif、ACCGAC-motif	ABA响应元件 ABA response element
		TGA-element	IAA响应元件 IAA response element
		MYC	干旱响应元件 Drought response elements
JA	CYP81D11	TGACGTCA-motif	结合TGA转录因子Binding to TGA transcription factors	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Köster et al.,2012
	CYP81D11	as-1、G-box	结合JA调节的转录因子Transcription factors regulated by JA binding	拟南芥 Arabidopsis thaliana	拟南芥 Arabidopsis thaliana	GUS	Köster et al.,2012
	TSA1	/	/	拟南芥 Arabidopsis thaliana	拟南芥、烟草 Arabidopsis thaliana，Nicotiana tabacum	GFP	Geem et al.,2019
	Zmap	CGTCA-motif、TGACG-motif	MeJA响应元件 MeJA response elements	玉米 Zea mays	烟草 Nicotiana tabacum	GUS	Jin et al.,2019
		GGTCCAT-motif	IAA响应元件 IAA response elements
		Box-II、GA-motif	光响应元件 Light response elements
		H-box、MBS	ABA、干旱响应元件ABA and drought response elements
	VvPR1	ACGTG-motif、MBS	ABA、干旱响应响应元件 ABA and drought response elements	葡萄 Vitis vinifera	烟草 Nicotiana tabacum	GUS	Rahman et al.,2022b
		ERE	ETH响应元件					ETH and drought response elements
		P-box	GA响应元件 GA response elements
		TCA	SA反应元件 SA response elements
		CGTCA-motif	MeJA响应元件 MeJA response elements
		LS7、G-box、AT1-motif	光响应元件 Light response elements
	TkSRPP	CGTCA-motif	MeJA响应元件 MeJA response elements	蒲公英 Taraxacum officinale	烟草 Nicotiana tabacum	GUS	Dong et al.,2023
		TGA-element	IAA响应元件 IAA response elements
		W-box、TTGAC-motif	SA响应元件 SA response elements
		CANNTG-motif、CNGTTR-motif、ACTG-motif	ABA响应元件 GA response elements
		MBS	干旱响应 Drought response

Advances in Plant Promoter Research

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 164

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yuxiang, HAN Fengqing, ZHAO Xinyu, LIU Yumei, LI Zhansheng, $\boxed{\hbox{FANG Zhiyuan}}$. Cloning and Functional Analysis of Lateral Branching Regulatory Gene BoBRC1 in Broccoli [J]. Acta Horticulturae Sinica, 2024, 51(9): 1997-2007.
[2]	WANG Chenyu, LIU Mengjun, WANG Lixin, LIU Zhiguo. Research Progress of CRISPR/Cas9 Technology and Its Application in Horticultural Plants [J]. Acta Horticulturae Sinica, 2024, 51(7): 1439-1454.
[3]	JIANG Rui, ZHOU Shengjun, ZHU Yuqiang, WANG Xin, TAN Jihong, WANG Huasen, ZHANG Peng. Research Progress of CRISPR/Cas9 Gene Editing Techniques in Melon Crops [J]. Acta Horticulturae Sinica, 2024, 51(7): 1683-1694.
[4]	LI Qinqin, DONG Shanrong, LUO Jianrang, ZHANG Yanlong. Cloning and Functional Analysis of PqDFR and PqANS Genes and Its Promoters from Paeonia qiui [J]. Acta Horticulturae Sinica, 2024, 51(6): 1256-1272.
[5]	MA Cunfa, WU Ting, ZHAO Hui, ZHANG Tianpei, ZHAO Lijian, YU Yonghui, XIAO Jiancheng, LI Jun, WU Shuiqin. Creation of Self-compatible Broccoli Lines by Knocking out BoSP11 Gene Through CRISPR/Cas9 Technology [J]. Acta Horticulturae Sinica, 2024, 51(3): 509-519.
[6]	FENG Zhijuan, LIU Na, ZHANG Guwen, BU Yuanpeng, WANG Bin, GONG Yaming. Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA [J]. Acta Horticulturae Sinica, 2024, 51(12): 2791-2799.
[7]	YE Yufan, WANG Yujie, FU Qianyuan, WANG Lu, HAO Xinyuan, DING Changqing, WANG Xinchao, CAO Hongli, LI Nana. Cloning and Expression Analysis of Mg-Chelatase H Subunit Gene CsChlH in Tea Plant（Camellia sinensis） [J]. Acta Horticulturae Sinica, 2024, 51(1): 91-102.
[8]	LI Songqi, LI Xufei, LI Min, LIU Hainan, PEI Maosong, WEI Tonglu, GUO Dalong, YU Yihe. Cloning，Expression and Promoter Activity Analysis of VlRRA1 Gene in Grape [J]. Acta Horticulturae Sinica, 2023, 50(8): 1609-1621.
[9]	LIU Hui, YIN Xiangjing, FANG Jinghao, GAO Min, LI Zhi, WANG Xiping. Cloning and Functional Analysis of the CDS and Promoter of Synthase Gene STS19 in Chinese Wild Grapevine [J]. Acta Horticulturae Sinica, 2023, 50(7): 1389-1401.
[10]	YANG Mengxia, LIU Xiaolin, CAO Xue, WEI Kai, NING Yu, YANG Pei, LI Shanshan, CHEN Ziyue, WANG Xiaoxuan, GUO Yanmei, DU Yongchen, LI Junming, LIU Lei, LI Xin, HUANG Zejun. Construction and Application of a CRISPR/Cas9 System for Multiplex Gene Editing in Tomato [J]. Acta Horticulturae Sinica, 2023, 50(6): 1215-1229.
[11]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[12]	XIONG Wenwen, LIU Huimin, CHEN Kaiyuan, DU Juan, SONG Botao, JING Shenglin. Optimization of Gene Editing System Based on Heat-treated CRISPR/Cas9 Potato Plants [J]. Acta Horticulturae Sinica, 2023, 50(12): 2591-2600.
[13]	YUAN Xin, XU Yunhe, ZHANG Yupei, SHAN Nan, CHEN Chuying, WAN Chunpeng, KAI Wenbin, ZHAI Xiawan, CHEN Jinyin, GAN Zengyu. Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit [J]. Acta Horticulturae Sinica, 2023, 50(1): 53-64.
[14]	ZUO Xin, LI Mingming, LI Xinrong, MIAO Chunyan, LI Yanfang, YANG Xu, ZHANG Zhongyi, WANG Fengqing. CRISPR/Cas9 Technology for RcPDS1 Gene Editing in Rehmannia chingii [J]. Acta Horticulturae Sinica, 2022, 49(7): 1532-1544.
[15]	WANG Dan, WANG Mi, LIU Jun, ZHOU Xiaohui, LIU Songyu, YANG Yan, ZHUANG Yong. Cloning of U6 Promoters and Establishment of CRISPR/Cas9 Mediated Gene Editing System in Eggplant [J]. Acta Horticulturae Sinica, 2022, 49(4): 791-800.