Analysis of Floral Bud Differentiation and Related Genes Expression Under Different Temperatures in Tulipa gesneriana

doi:10.16420/j.issn.0513-353x.2022-0091

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (5): 1037-1047.doi: 10.16420/j.issn.0513-353x.2022-0091

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Analysis of Floral Bud Differentiation and Related Genes Expression Under Different Temperatures in Tulipa gesneriana

CHEN Xinchen¹^,², ZHAO Huimin¹, WANG Sen¹^,², WU Simin¹^,², XIANG Lin¹, CHAN Zhulong¹, WANG Yanping¹^,^*()

¹National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops，College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China
²National R & D Centre for Citrus Preservation，Wuhan 430070，China

Received:2022-12-29 Revised:2023-02-03 Online:2023-05-25 Published:2023-05-31
Contact: WANG Yanping E-mail:ypwang@mail.hzau.edu.cn

Abstract

Abstract:

In this study，the cultivar‘World’s Favourite’was used to compare the processes of floral bud differentiation and floral organ formation under different temperature conditions，as well as a comparison of plant growth and flowering after bulbs planting. The results showed that temperature had a significant effect on the floral buds differentiation and formation of complete flower bud structure. 22 ℃ is the most suitable temperature for floral bud differentiation under which the differentiation speed is the fastest and the floral bud structure is intact. Bulbs under 28 ℃ could form normal floral bud and displayed a faster growth，while the complete floral organ structure failed to be formed under 5 ℃ condition. The expression patterns of genes related to flower development and flowering time regulations were further analyzed by qRT-PCR. The results showed that ABC family genes and flowering time related genes were significantly different in floral buds differentiated under different temperatures. Further cloning and functional exploration of the class A gene TgSQA demonstrated that it encoded 246 amino acids with a typical MADS-box and K-box domain. TgSQA over-expressed transgenic Arabidopsis thaliana obtained an early flowering phenotype compared with the wild type，indicating that TgSQA was a positive regulator during flowering time regulation.

Key words: Tulipa gesneriana, temperature, floral bud differentiation, flowering regulation, TgSQA

CLC Number:

S682.263

CHEN Xinchen, ZHAO Huimin, WANG Sen, WU Simin, XIANG Lin, CHAN Zhulong, WANG Yanping. Analysis of Floral Bud Differentiation and Related Genes Expression Under Different Temperatures in Tulipa gesneriana[J]. Acta Horticulturae Sinica, 2023, 50(5): 1037-1047.

Figures/Tables 8

References 28

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引物名称 Primer	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
TgSQA	GGCAGACTGAAGGCTAAGGT	GCTCGTTGAAGATCGGCAAT
TgSQB	GGAGCAGAAGTCCAAGGCTT	GCTCCATCCTCTTCGTTGCT
TgSEP1	TGCCCTTCGACTATCATGGG	CAGGAGCATAGCCATCACCA
TgFT-like	GTGGATCCTGATGCTCCGAG	AAAACAAACACGAGGCGGTG
TgSPL2	CCAGAAATGTCGGAGCCCAG	AGATGGAACCTGCTGCACTG
TgGLO	GAACTGCGGGAACAAGCTCT	AATCGGGATCAGCTCCTTGG
TgLFY	ATTACGTCCACTGCTACGCC	GGGCTGAGATTGCAACGAGA
TgSOC1-like1	AGAGGGTTTGGTAGAGCAAAGT	GGTCTGATGCGGGGATTCTT
TgSOC1-like2	TCACAGCTCAGAGGAAAGGA	GCAAGTCCGTCTCCACATCT
TgTFL1	GGCAGAGGAGAGGACAGGTA	AAGAGAGTGTCAGTCAGCGG
TgFLK	TGCCTTGCAAGACGATAGGG	ATGCGGTGGATGTTGCATTG
TgVIN3	CGACCATCCAGTTCGACTCC	AGCAATCTTCCTCGCGTGAT
TgUBQ10	GTCGCACGTTGGCTGATTAC	GGGCTTAAAGACCACCACGA
AtUBQ10	CCAAGATCCAAGACAAAGAGGG	TGAGAACAAGATGAAGGGTGG

引物名称 Primer	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
TgSQA	GGCAGACTGAAGGCTAAGGT	GCTCGTTGAAGATCGGCAAT
TgSQB	GGAGCAGAAGTCCAAGGCTT	GCTCCATCCTCTTCGTTGCT
TgSEP1	TGCCCTTCGACTATCATGGG	CAGGAGCATAGCCATCACCA
TgFT-like	GTGGATCCTGATGCTCCGAG	AAAACAAACACGAGGCGGTG
TgSPL2	CCAGAAATGTCGGAGCCCAG	AGATGGAACCTGCTGCACTG
TgGLO	GAACTGCGGGAACAAGCTCT	AATCGGGATCAGCTCCTTGG
TgLFY	ATTACGTCCACTGCTACGCC	GGGCTGAGATTGCAACGAGA
TgSOC1-like1	AGAGGGTTTGGTAGAGCAAAGT	GGTCTGATGCGGGGATTCTT
TgSOC1-like2	TCACAGCTCAGAGGAAAGGA	GCAAGTCCGTCTCCACATCT
TgTFL1	GGCAGAGGAGAGGACAGGTA	AAGAGAGTGTCAGTCAGCGG
TgFLK	TGCCTTGCAAGACGATAGGG	ATGCGGTGGATGTTGCATTG
TgVIN3	CGACCATCCAGTTCGACTCC	AGCAATCTTCCTCGCGTGAT
TgUBQ10	GTCGCACGTTGGCTGATTAC	GGGCTTAAAGACCACCACGA
AtUBQ10	CCAAGATCCAAGACAAAGAGGG	TGAGAACAAGATGAAGGGTGG

温度/℃ Temperature	花芽长度/mm Length of floral bud			花芽宽度/mm Width of floral bud
温度/℃ Temperature	06-10（10 d）	06-28（28 d）	07-14（44 d）	06-10（10 d）	06-28（28 d）	07-14（44 d）
5	2.00 ± 0.44 b	3.17 ± 0.61 b	5.33 ± 0.72 b	1.90 ± 0.17 b	1.97 ± 0.25 b	2.37 ± 0.55 b
22	3.68 ± 0.44 a	6.78 ± 1.72 a	8.83 ± 2.40 a	2.76 ± 0.43 a	3.00 ± 0.41 a	3.88 ± 0.85 a
28	1.74 ± 0.19 b	2.60 ± 0.66 b	3.23 ± 0.21 c	1.54 ± 0.21 c	1.95 ± 0.62 b	2.28 ± 0.50 b

温度/℃ Temperature	花芽长度/mm Length of floral bud			花芽宽度/mm Width of floral bud
温度/℃ Temperature	06-10（10 d）	06-28（28 d）	07-14（44 d）	06-10（10 d）	06-28（28 d）	07-14（44 d）
5	2.00 ± 0.44 b	3.17 ± 0.61 b	5.33 ± 0.72 b	1.90 ± 0.17 b	1.97 ± 0.25 b	2.37 ± 0.55 b
22	3.68 ± 0.44 a	6.78 ± 1.72 a	8.83 ± 2.40 a	2.76 ± 0.43 a	3.00 ± 0.41 a	3.88 ± 0.85 a
28	1.74 ± 0.19 b	2.60 ± 0.66 b	3.23 ± 0.21 c	1.54 ± 0.21 c	1.95 ± 0.62 b	2.28 ± 0.50 b

Analysis of Floral Bud Differentiation and Related Genes Expression Under Different Temperatures in Tulipa gesneriana

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